Dna Replication, Repair, And Recombination

Front 1

True or False: The conservative model of DNA replication is supported by experimental evidence.

Back 1

False

Front 2

True or False: In E. coli, replication is unidirectional.

Back 2

False

Front 3

True or False: Bacterial replication requires dAMP, dTMP, dGMP, and dCMP.

Back 3

False

Front 4

True or False: Both eukaryotic and prokaryotic cells have several different DNA polymerases that are involved in replication and repair of DNA.

Back 4

True

Front 5

True or False: Kornberg's enzyme (DNA polymerase I) catalyzes the synthesis of DNA if provided with only the four deoxyribonucleoside triphosphates.

Back 5

False

Front 6

True or False: DNA polymerase III performs the major role in prokaryotic replication.

Back 6

True

Front 7

True or False: DNA polymerase III requires only a single-stranded DNA template for replication.

Back 7

False

Front 8

True or False: The reaction by which deoxyribonucleotides are added during replication is a near-equilibrium process.

Back 8

False

Front 9

True or False: Eukaryotic Okazaki fragments are smaller than prokaryotic Okazaki fragments.

Back 9

True

Front 10

True or False: DNA ligase plays a role in normal DNA replication as well as in repair of damaged DNA.

Back 10

True

Front 11

The location at which duplex DNA is unwound during replication and at which new DNA is being synthesized is called the ___.

Back 11

replication fork

Front 12

During replication, proteins that bind to individual unwound DNA strands to prevent them from re-forming as a double helix are called ___.

Back 12

single-strand binding proteins

Front 13

The DNA strand that serves as a complementary pattern along which a new DNA strand is synthesized is called the ___ strand.

Back 13

template

Front 14

Short segments of RNA required for synthesis of DNA on a template are called ___ which are removed from the product DNA by the ___ activity of DNA polymerase I.

Back 14

primers; 5'-->3' exonuclease

Front 15

The DNA strand formed as a continuous strand during replication is called the ___ and the strand formed in a discontinuous pattern is called the ___.

Back 15

leading strand; lagging strand

Front 16

DNA sequencing is best done by using ___ to effect termination of the growing DNA molecule, followed by ___ to separate the resulting DNA fragments to determine their size.

Back 16

dideoxyribonucleotides; electrophoresis

Front 17

Short segments of lagging-strand DNA formed by the discontinuous process are called ___.

Back 17

Okazaki fragments

Front 18

___ is the name of the process by which DNA polymerase I catalyzes the formation of DNA segments to replace the RNA primer segments required by DNA polymerase III during replication.

Back 18

Nick translation