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10 Cards in this Set
- Front
- Back
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DNA Rep II Eukaryotes
Where is DNA found in Eukaryotes? |
Nuclei and mitochondria
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DNA Rep II Eukaryotes
How is DNA packaged? |
30nm fibers of nucleosomes which are coiled and supercoiled to form chromosomes
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DNA Rep II Eukaryotes
Which DNA Polymerase is believed to be responsible for leading strand synthesis? |
DNA Polymerase Delta
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DNA Rep II Eukaryotes
Which DNA Polymerase is believed to be responsible for lagging strand synthesis? |
DNA Polymerase Alpha
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DNA Rep II Eukaryotes
What proteins are in nucleosomes, and why do they bind to DNA? |
Histones.
Positively charged Histone is attracted to negatively charged DNA molecules |
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DNA Rep II Eukaryotes
What is PCNA, and what roles does it play? |
Proliferating Cell Nuclear Antigen aka Cyclin.
Forms a clamp around the DNA strand to dramatically increase the processivity of DNA Polymerase Delta. |
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DNA Rep II Eukaryotes
What is the arrangement of nucleosomes after DNA Replication? |
Cooperative distribution of nucleosomes.
New histones end up along one daughter strand near each replication fork. |
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DNA Rep II Eukaryotes
How might nucleosomes affect eukaryotic DNA replication? |
Short Okazaki fragments and slow DNA polymerization
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DNA Rep II Eukaryotes
What is the model used to describe DNA replication in mitochondria? |
Maternal inheritance by D-Loop
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DNA Rep II Eukaryotes
What are some contrasts between features of prokaryotic and eukaryotic DNA replication? |
Eukaryotic
1) Greater length of DNA replication 2) Movement of replication fork is slower 3) Use a cell cycle limiting replication to a few hours 4) Large number of DNA Polymerases 5) Numerous sites of replication- bidirectional |
