Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
20 Cards in this Set
- Front
- Back
|
RPA
|
SSBs
(replication protein A) |
|
|
FEN1- extended name and what repair process is it involved in?
|
flap endonuclease
part of BER (2-6nt) |
|
|
PNK- what is extended name and what repair process is it involved in?
|
polynucleotide kinase
part of BER (bifunctional glycos) and NHEJ as 5' kinase/ 3'phosphatase |
|
|
Clamp loader for PCNA
|
RFC 1/5
|
|
|
Clamp loader for 911 complex
|
Rad 17/ RFC 2/5 (quarter piece!)
|
|
|
what nt does deamination convert?
|
C--->U
T----> 5mC |
|
|
What glycosylases repairs 5mC --->T?
|
TDG and MBD4
|
|
|
What is MutY?
|
glycosylase that removes 'A' from A/G, A/GO, A/C
also removes G from G/GO |
|
|
Describe general structure DNA glycosylases
|
-form helix-hairpin-helix
- CTD assoc with parent base - NTD asssoc with flipped out base/active site - linker region btw domains -glycos. bends DNA |
|
|
similarities/diff btw 911 and PCNA
|
similarities: symmetry, similar clamp loaders (RFC 1/5 for PCNA, Rad17/RFC 2/5 for 911)
differences: 911 has H2/3 in center, PCNA has smooth opening for DNA, PCNA- homotrimer, 911- heterotrimer |
|
|
Euk version of MutS
|
MSH2
|
|
|
Euk version of MutL
|
MSH1
|
|
|
Euk version of MutH
|
none
|
|
|
function of UrvD in what process
|
helicase II, used in MMR to unwind newly nicked DNA & in NER
|
|
|
Name enzymes involved in mismatch repair (MMR) and what they do
|
Mut S: bind mismatch and loops up to 4nt
Mut L: binds and stabilizes Mut S Mut H: nick nascent DNA on strand opposite from methylated (parent) DNA Urv D: helicase to unwind DNA from nick toward mismatch Pol III: fill gap Ligase: seals nick |
|
|
Name the components that make up MutS (alpha) and MutS (beta) in eukaryotes. What is the oligomerization of Mut S in pro and euk?
|
There are 2 versions of MutS in euk:
1. MSH2/6 (majority in cell)- MutS(alpha) 2. MSH 2/3- MutS(beta) **MutS in prokary- homodimer in euk- heterodimer |
|
|
What is euk version of Pol III? Euk version of clamp loader and beta clamp?
|
Pol (epsilson), PCNA/RFC
|
|
|
Name and describe proteins involved in eukaryotic MMR
|
-MutSalpha, MutLalpha: detects and binds mismatch
-RFC: clamp loader -PCNA: replication clamp -RPA: ssBP -exonuclease I:hydrolyzes the 5'-ended strand in dsDNA -polymerase delta: fill in the gap - ligase |
|
|
What are the components of the euk homolog of MutL?
|
MLH1/PMS2
|
|
|
How are the exonucleases in MMR different between prok and euk?
|
In prokaryotes:
-if nick 3' of mismatch, exoVII or RecJ -if nick 5' of mismatch, exo I or X In euk: only exo I regardless of where nick is but strangely, it has 5'-->3' exo but no 3'---> 5' exo |
