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17 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Sterilization
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refers to the removal of all forms of life, including bacterial spores.
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bearbrand 🍼
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Disinfectant
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chemical agents applied to inanimate objects.
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lysol
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Antiseptic
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a substance applied to the skin for eliminating or reducing the number of bacteria.
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alcohol
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Spores
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a type of organisms that coats rich in proteins, lipids and carbohydrates and cores rich in dipicolinic acid and calcium mycobacterial cell wall; rich in lipids (mycolic acid)
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smores
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Number of Organisms
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microbial load or bioburden
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quantity
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a. physical methods
b. chemical methods |
Methods of disinfection and sterilization
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changes
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Physical Methods
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Heat
Filtration Radiation |
sun, strainer, x-ray
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Chemical Methods
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alcohol
aldehydes halogens heavy metal detergent phenolics gases |
chemistry
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Boiling
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it destroys vegetative bacteria; 100 degrees celsius for 10-15mins
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moist heat procedure
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Autoclave
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it is a chamber which is filled with hot steam under pressure (steam under pressure); fastest and simpliest method of sterilization; all organisms and spores are killed within 15 mins
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moist heat procedure
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Autoclave
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Bacillus stearothermophilus
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biological indicators
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Oven
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Bacillus subtilis var niger
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biological indicators
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Ionizing Radiation
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Bacillus pumilus
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biological indicators
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Ethylene oxide
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Bacillus subtilis var globigii
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ethyl___ oxid_
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Fractional/Intermittent Sterilization (Tyndalization)
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Arnold's sterilizer (free flowing steam)
100 degrees celsius for 30mins (3 consecutive days) |
moist heat procedure
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Inspissation
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involves thickening through evaporation; is used to sterilize protein-rich medium such as Lowenstein Jensen Medium; 70-80 degrees celsius for 2hrs (3 consecutive days)
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moist heat procedure
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Inspissation
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involves thickening through evaporation; used to steriliz protein-rich medium such as Lowe stein Jensen Medium; 70-80 degrees celsius for 2hrs (3 consecutive days)
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Moist heat procedure
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