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20 Cards in this Set
- Front
- Back
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better than dry head because H2O speeds denaturing
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wet heat
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good for things that would be ruined by water - can kill spores at 160' for 2 hors
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dry heat (this is not an autoclave - an autoclave is wet heat)
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how do you test for the effectiveness of pasteurization
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loss of alkaline phosphatase activty - neg culture coxiella vurnetti
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pore size that exludes ALL bacteria
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.22 microns
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does not penetrate anything except air and thing layers of solid and liquid -
forms pyrimidine dimers in DNA damages human tissue |
nonionizing radiation
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alkylating agent - (lipids, proteins, DNA)
used for heat sensitive materials such as plastic |
ethylene oxide
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similar to ethyl alcohol but no legal restrictions, more potent, less volatile
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isopropanol
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antiseptic on skin
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iodine
tincture = iodine + KI in H2O or ethanol |
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used in water supply
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chlorine
hypochlorite is used to clean surfaces |
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cationic detergents destroy
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membranes and dissolve lipids
drawn towards neg charge on bacterial and dissolves film that may protect bacteria |
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leaves bacteriocidal film on treated objects
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cationic detergents
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inactivated by catalase
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hydrogen peroxide (an oxidizing agent)
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useful against anaerobes
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oxidizing agenst (hydrogen peroxide)
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denatures proteis - may required high conc
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phenols
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triclosan is in
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antibacterial soap
(a phenol) |
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protects against gnococcus
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sliver nitrate (a heavy metal)
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binds SH groups
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mercury compouds
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used in vaccines
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formaldehyde
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gluteraldehyde is used to clean
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synringes and blood
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preservatives can fall into what three categories
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1. short chain fatty acids
2. organic acids 3. phenolics (smoked food) |
