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24 Cards in this Set
- Front
- Back
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What traits are universal for Enterobacteriaceae?
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G- rods
do not form spores ferment glucose catalase + oxidase - nitrate + |
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What is an endotoxin?
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part of G- cell walls, consists of 3 layers: outer variable carbohydrate (det o-antigen specificity), middle core polysaccharide, central highly conserved lipid moiety, lipid A.
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What three antigens are used to characterize Enterobacteriaceae?
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Flagellar H antigens: heat labile, IgG antibody resp
Capsular K antigens: heat labile, protein or polysac Somatic O antigens: compose outer region of LPS, heat stable, IgM antibody resp |
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There are 7 tribes in Enterobacteriaceae, what are they?
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Tribe I- Eschericheae (Escherichia, Shigella)
Tribe II- Edwardsielleae (Edwardsiella) Tribe III- Salmonelleae (Salmonella) Tribe IV- Citrobactereae (Citrobacter) Tribe V- Klebsielleae (Klebsiella, Raoultella, Enterobacter, Hafnia, Serratia, Pantoea) Tribe VI- Proteeae (Proteus, Providencia, Morganella) Tribe VII- Yersinieae (Yersinia) |
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Describe the carbohydrate utilization test
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-results in production of pyruvic acid from glucose
-to utilize lactose, must have beta galactoside permease and beta galactosidase -use ONPG test, cleaves bond - + test is yellow |
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What are rxns of KIA?
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no ferm = alk/alk
glucose ferm = alk/acid glucose ferm & H2S = alk/acid/black glucose/ lactose ferm = acid/acid |
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Describe MacConkey Agar
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bile salts and aniline dyes inhibit G+ bacteria
strong lactose ferm = red weak lact ferm = pink non ferm = colorless |
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Describe Eosin Methylene Blue Agar
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strong lac ferm = green/blk with metallic sheen
weak lac ferm = purple non ferm = purple-blk |
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Describe Hektoen Agar
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strong lac ferm = orange/pink
salmonella = blue/grn w/ blk centers shigella = green |
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Describe XLD agar
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xylose lysine dexycholate agar
contains xylose, lactose, lysine, sucrose E.coli appears yellow |
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Describe Selenite Broth
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used for recovery of salmonella and shigella, inhibits E.coli
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Describe Indole test
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indole = degradation product from breakdown of tryptophan via tryptophanase
detected through addition of Kovac's reagent. RED = + |
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Describe Methyl Red test
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ferm of glucose produces enough acid to maintain ph below 4.4
Acid:Neutral 4:1 CO2:H2 1:1 RED = + |
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Describe Voges-Proskauer test
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bacteria break down glucose through butanediol ferm pathway, produce acetoin as intermediate. this test converts acetion to diacetyl via KOH, diacetyl converted to red complex via alpha naphthol and creatine
Acid:Neutral 1:6 CO2:H2 5:1 RED = + |
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Describe citrate utilization test
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strain utilizes citrate as sole carb source
BLUE = + |
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what is the IMViC test?
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Indole, methyl red, voges-proskauer, citrate
E.coli is ++-- E. aerogenes is --++ |
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Describe the urease production test
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bact hydrolyze urea into ammonia
RED/PINK = + |
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Describe the decarbox of AA test
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bact remove a molecule of CO2 from spec AA
lysine -> cadaverine ornithine -> putrescine arginine -> citrulline YELLOW = + (acidification of bromocresol purple indicator) |
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Describe the phenylalanine deaminase production test
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bact possess enzyme for the oxidative deamination of phenylalanine
phenylpyruvate is detected after addition of ferric chloride GREEN = + |
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Describe H2S production test
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ferrous sulfate is the indicator
BLACK PPT = + |
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What bacteria are H2S+?
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Edwardsiella tarda, Salmonella sp., Citrobacter freundii, Proteus vulgaris, Proteus mirabilis
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What bacteria are VP+?
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Klebsiella sp., Enterobacter sp., Hafnia sp., Pantoea sp., Serratia sp.
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What bacteria are PAD+?
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Proteus sp., Morganella sp., Providencia sp.
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What bacteria are nonmotile?
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Shigella sp., Klebsiella sp.
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