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13 Cards in this Set
- Front
- Back
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where is casein mRNA expressed?
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mammary glands
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what happens to casein mRNA in the prescence of prolactin?
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-> increases -> half life increases -> PolyA tail length increased -> 3'UTR binds stabalising proteins |
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enzymes that remove m7G cap?
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-> decapping enzymes -> DCP1 -> DCP2 |
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endonucleases
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-> argonaute -> Swt1 ->Smg6 |
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deadenylases
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-> remove polyA tail ->Ccr/Not complex |
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what are the nuclease activities in the exosome?
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-> RRP6 -> RRP44 |
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deadenylation-dependant decay pathway
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1) polyA tail is degraded by CCR4-NOT or PARN 2) 3' to 5' decay 3) stimulation of decapping by DCP2 4) XRN1 comes in to degrade body of mRNA |
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what does the ARE do?
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-> binds to AU-rich binding proteins -> promotes decay by interacting with CCR4-NOT |
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what does the c-fos major coding determinant do?
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-> binds to a complex of proteins. -> stabilizes mRNA at first -> when mRNA is translated complex gets pushed away. -> de-stabalised |
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Deadenylation-independant decay pathway
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1) Rps28B binds to sequence on 3'UTR 2) binds its own message -Edc3 3) activates de-capping enzymes in the cell. 4) opens up 5' to 3' to XRN1 |
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how are siRNAs made?
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-> in the cytoplasm from dsRNA -> processed by enzymes= DICER -> have a 3'overhang |
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how is miRNA made?
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-> encoded in genome -> processed by Drosha -> Exportin removes them from nucleus -> processed by DICER into duplex |
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where does miRNA tend to bind?
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3'UTR
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