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8 Cards in this Set
- Front
- Back
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Two questions that led to historical development
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Does spontaneous generation occur?
What is the nature of contagious disease? |
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Spontaneous Generation
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Does life arise from nonliving matter (abiogenesis) or only from living matter (biogenesis)?
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Koch's Postulates
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1. Suspected pathogen should be present in all cases
2. Organism should be grown in pure culture 3. Cells from pure culture should cause disease in a healthy host 4. Pathogen should be reisolated and shown to be the same as the original |
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Problems with studying microorganisms
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- They are invisible and widely distributed
- Usually need to grow under controlled situations in lab - Strains must be separated |
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Inoculation
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Introduction of small sample into a medium for growth
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Culture
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the observable growth
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Medium
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A nutrient solution--solid, liquid, or semi-solid, used to grow microorganisms
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Media
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- Liquid
- Solid - Semi-solid Agar is not a food source, it's a hardener that comes from seaweed - Defined (synthetic) and Undefined (complex) - Selective and differential (MacKonkey + Mannitol Salts are both) |
