Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
29 Cards in this Set
- Front
- Back
|
1. Purpose of: Eosin Methylene Blue (EMB)?
|
a. Isolate of gram negative
|
|
|
2. Nutrients/Food for bacteria isolated by: Eosin Methylene Blue (EMB)?
|
a. Peptone
b. Sucrose c. Lactose |
|
|
3. Inhibitors of (if applicable): Eosin Methylene Blue (EMB)?
|
a. Methylene Blue
b. Eosin Y |
|
|
4. Indicators (i.e. ph, if applicable): Eosin Methylene Blue (EMB)?
|
a. none
|
|
|
5. Reactions/Growth results for: Eosin Methylene Blue (EMB)?
|
a. Poor Growth or no growth
i. Organism is inhibited by eosin and methylene blue ii. Gram positive b. Good growth i. Organism is not inhibited by eosin and methylene blue ii. Gram negative c. Growth is pink and mucoid i. Organism ferments lactose with little acid production ii. Possible coliform d. Growth is “dark” (purple to black with or without green metallic sheen) i. Organism ferments lactose and/or sucrose with acid production ii. Probable coliform e. Growth is “colorless” (no pink, purple, or metallic sheen) i. Organism does not ferment lactose or sucrose ii. Noncoliform |
|
|
6. Other components of: Eosin Methylene Blue (EMB)?
|
a. Dipotassium phosphate
|
|
|
7. Purpose of: Hektoen Enteric Agar (HE)?
|
a. Isolate and differentiate shigella and salmonella from other gram negative enteric organisms
|
|
|
8. Nutrients/Food for bacteria isolated by: Hektoen Enteric Agar (HE)?
|
a. Lactose
b. Sucrose c. Salicin d. Yeast extract |
|
|
9. Inhibitors of (if applicable):
|
a. Bile salts
|
|
|
10. Indicators (i.e. ph, if applicable): Hektoen Enteric Agar (HE)?
|
a. none
|
|
|
11. Reactions/Growth results for: Hektoen Enteric Agar (HE)?
|
a. Poor or no growth
i. organism is inhibited by bile and/or one of the dyes included ii. gram positive b. Good growth i. organism is not inhibited by bile and/or any of the dyes included ii. gram negative c. Pink to orange growth i. organism produces acids from lactose fermentation ii. not shigella or salmonella d. Blue-green growth with black precipitate i. Organism does not ferment lactose, but reduces sulfur to hydrogen sulfide ii. Possible salmonella e. Blue-green growth without black precipitate i. organism does not ferment lactose or reduce sulfur ii. possible shigella or salmonella |
|
|
12. Other components of: Hektoen Enteric Agar (HE)?
|
a. Peptic digest of animal tissue
b. Ferric ammonium citrate c. Acid fushin d. Bromythmol blue e. Sodium thiosulfate |
|
|
13. Purpose of: MacConkey agar?
|
a. Isolate and differentiate members of the Enterobacteriaceae
|
|
|
14. Nutrients/Food for bacteria isolated by: MacConkey agar?
|
a. Pancreatic digest of gelatin
b. Pancreatic digest of casein c. Lactose |
|
|
15. Inhibitors of (if applicable): MacConkey agar?
|
a. Bile salts
b. Crystal violet |
|
|
16. Indicators (i.e. ph, if applicable): MacConkey agar?
|
a. Neutral Red
i. Dye that is colorless at pH above 6.8 and red at pH below 6.8 |
|
|
17. Reactions/Growth results for: MacConkey agar?
|
a. Poor growth or no growth
i. Organism is inhibited by crystal violet and/or bile ii. Gram positive b. Good growth i. Organism is not inhibited by crystal violet or bile ii. Gram negative c. Pink to red growth with or without bile precipitate i. Organism produces acid from lactose fermentation ii. Probable coliform d. Growth is “colorless” (not red or pink) i. Organism does not ferment lactose, NO REACTION ii. Noncoliform |
|
|
18. Other components of: MacConkey agar?
|
a. Sodium chloride
|
|
|
19. Purpose of: Mannitol Salt Agar?
|
a. Isolation and differentiation of Staphylococcus aureus
|
|
|
20. Nutrients/Food for bacteria isolated by: Mannitol Salt Agar?
|
a. Peptone
b. Phenol red c. Carbohydrate nutrient d. Mannitol |
|
|
21. Inhibitors of (if applicable): Mannitol Salt Agar?
|
a. Sodium chloride
b. Salt content |
|
|
22. Indicators (i.e. ph, if applicable): Mannitol Salt Agar?
|
a. Phenol red
i. pH > 8.4 –pink ii. pH<6.8 yellow |
|
|
23. Reactions/Growth results for: Mannitol Salt Agar?
|
a. Poor growth or no growth
i. Organism is inhibited by NaCl ii. Not staphylococcus b. Good growth i. Organism is not inhibited by NaCl ii. staphylococcus c. Yellow growth or halo i. Organism produces acid from mannitol fermentation ii. Possible pathogenic staphylococcus aureus d. Red growth (no halo) i. Organism does not ferment mannitol. NO REACTION ii. Staphylococcus other than S. aureus |
|
|
24. Other components of: Mannitol Salt Agar?
|
a. Beef extract
|
|
|
25. Purpose of: Urea test?
|
a. Differentiate organisms based on their ability to hydrolyze urea with the enzyme urease.
|
|
|
26. Nutrients/Food for bacteria isolated by: Urea test?
|
a. Peptone
b. Glucose c. Urea d. Potassium phosphate |
|
|
27. Inhibitors of (if applicable): Urea test?
|
a. None
|
|
|
28. Indicators (i.e. ph, if applicable): Urea test?
|
a. Phenol red
|
|
|
29. Reactions/Growth results for: Urea test? (culture)
|
a. Urease Agar:
i. 24 hours ii. All pink no change in 6 days 1. Rapid urea hydrolysis; strong urease production iii. Partially pink change to all pink or same in 6 days 1. slow urea hydrolysis; weak urease production iv. Orange or yellow changes to all pink or partially pink in 6 days 1. slow urease hydrolysis; weak urease production v. Orange or yellow no change in 6 days 1. no urea hydrolysis; urease is absent |
