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51 Cards in this Set
- Front
- Back
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Dyes, antimicrobials, and salts are examples of selective agents used for what type of media?
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selective
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PEA agar is an example of what type of media
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Selective. It inhibits gram - microorganisms
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Differential media distinguishes
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Microorganisms that have a defined metabolic activity over others that lack it
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How do you know if anything changes for a differential media culture?
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It has an observable change such as pH color change.i.e. simmons citrate agar (green to blue)
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Selective/differential media example
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mannitol salt agar
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Mannitol salt agar medium selective/differential
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Salt will inhibit all growth except for staphylococcus species
Differential only the aureus sp. |
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PEAB - Phenylethyl alcohol agar with blood
supports gram + or gram - |
gram +
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Columbia blood agar inhibits...
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gram -
CNA (Colistin, naladixic acid) |
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mannitol salt agar is selective for staphylococci which the aureus sp ferments....
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mannitol, which drops the pH turning the area yellow.
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Why is staphylococci selected on mannitol salt agar media
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b/c salt tolerant
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Tinsdale medium primarily isolates what?
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corynebacterium diptheriae
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Tinsdale medium is based off what?
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thiosulfate-tellurite
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How do corynebacterium appear?
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black colonies surrounded by dark gray halos
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Why does tinsdale medium isolate corynebacterium diptheriae?
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b/c potassium-tellurite is inhibitory to gram + and many gram - except for corynebacterium diptheriae
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Which medium is rich in phospholipids?
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Egg yolk medium
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What is differentiated out of egg yolk medium?
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Clostridium sp. with lecithinase activity
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What does the lecithinase do in the egg yolk medium?
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degrades phospholipids into insoluble diglycerides
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Thayer martin agar is based off?
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blood
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Thayer martin agar is selective for
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Neisseria gonorrhoeae
Neisseria meningitidis |
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Thayer martin agar contaminating microorganisms are inhibited by
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colistan, nystatin, vancomycin, trimethoprim, lincomycin
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In MacConkey agar medium, e-coli ferments what?
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lactose
red indicator changes to dark pink/red |
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EMB Agar contains what and differentiates what?
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Lactose
Lactose fermenters/non-fermenters |
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EMB Agar medium results in...
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metallic green colonies (fermentation of lactose)
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E-coli is a lactose fermenter or lactose non-fermenter?
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fermenter
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Hektoen Enteric Agar selects?
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Salmonella and shigella
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In hektoen enteric agar, the bile salts inhibit gram + and some gram - except for...
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salmonella and shigella
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EMB Agar contains what and differentiates what?
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Lactose
Lactose fermenters/non-fermenters |
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EMB Agar medium results in...
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metallic green colonies (fermentation of lactose)
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E-coli is a lactose fermenter or lactose non-fermenter?
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fermenter
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Hektoen Enteric Agar selects?
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Salmonella and shigella
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In hektoen enteric agar, the bile salts inhibit gram + and some gram - except for...
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salmonella and shigella
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Are salmonella and shigella fermenters or non fermenters?
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non-fermenters
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xylose lysine desoxycholate agar isolates?
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Salmonella and shigella
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What distinguishes salmonella from shigella?
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H2S production, xylose fermentation, lysine decarboxylation
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Bordet-gengou (BG) agar is based on?
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potato glycerol
Defibrinated blood |
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What is BG agar selective for?
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Bordetella pertusis (whooping cough)
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Bordetella pertusis pathogenecity?
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Exotoxin
Tracheal Cytotoxin Hemoagglutin |
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BCYE Agar medium is made of
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Yeast extract, charcoal and salts
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BCYE agar is selective for
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Legionella sp.
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TCBS Agar selects and differentiates
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Vibrio sp.
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What is the causative agent for cholera, diarrhea, food poisoning?
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Vibrio sp.
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CIN (cefsulodinlrgasan-novobiocin) agar selects for?
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Yersinia Sp.
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What appears as colonies with red pigmented "bullseye" in the middle of each colony?
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Yersinia
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Lowenstein Jensen Agar is based off?
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Egg
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lowenstein-jensen agar selects for?
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mycobacterium
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Middle brook agar is similar to lowenstein-jensen agar how?
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Both contain malachite green
Both select for mycobacterium |
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How is middle brook agar different then lowenstein-jensen?
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Its more chemically defined
OADC, stimulates egg components |
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MES Ureaplasma agar isolates?
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ureaplasma urealyticum
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What does MES agar contain?
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horse serum
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What is the significance of horse serum in MES agar?
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It stabilizes micro-organisms that lack a cell wall
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What does the MES agar colony look like?
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Fried egg
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