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442 Cards in this Set

  • Front
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What does FVCRP stand for?
-FVR-Feline Viral Rhinotracheitis (Virus)

-C-Calici Virus
-P-Panleukopenia (virus)
What is added when it is FVRCPC?
Chlamydia (bacteria)
What does DHLPP stand for? Are the diseases this contains viral or bacterial?
Distemper (viral)
Hepititis/Adenovirus (viral)
Leptospirosis (bacterial)
Parainfluenza (Viral)
Parvo (viral)
What if it was DHLPPC? What has been added?
Corona (virus)
What is the normal temperature for dogs and cats?
101.5 +/- degrees F
What is the normal respiration rate for dogs?
15-30 breaths per minute

(should count for 15 secs and multiply by 4. Any short than 15 secs tends to be less accurate)
What is the normal respiration rate for cats?
20-30 Seconds
What is the normal pulse rate for adult dogs? How does it differ for toy breeds?

Puppies?
60-160 bpm

Toy breeds-up to 180 bpm

Puppies-up to 220 bpm
What is the normal pulse rate for cats?
110-220 bpm
Define palliative
relieving or soothing symptoms of a disease without curing
Define anemia
decrease of deficiency of RBC's and hemoglobin in the blood due to loss, destruction, or lack of production.
Define immunosuppression
reduced response by the bodies immune system
Define Active immunity
immunity that results from the bodies own response to an antigen. (best kind!)
Define passive immunity
Immunity produced by administration of PRE-FORMED antibodies.

-Artificial-through injection of plasma, serum

-Maternal-ingestion of colostrum
Define acquired immunity
Antigen specific immunity attributable to production of antibodies and of specific immune T-lymphs.
Define diagnosis
Complete knowledge. Art & science of distinguishing one disease from another.
In regards to Specific Resistance vs Non-Specific Resistance:

What type of immunity is immediate?
Non-specific resistance
In regards to Specific Resistance vs Non-Specific Resistance:

What type of immunity includes antibody production?
Specific resistance
In regards to Specific Resistance vs Non-Specific Resistance:

What type of immunity protects against anything?
Non-specific resistance
In regards to Specific Resistance vs Non-Specific Resistance:

What type of immunity forms long term protection?
Specific resistance
What is the vertebral formula for dogs & cats?
C7 T13 L7 S3 CY20-23
What is the vertebral formula for horses?
C7 T18 L6 S5 Cy 15-21
What is the deciduous dental formula for dogs?
2(Di3/3 Dc1/1 Dp3/3) = 28 teeth
What is the adult dog dental formula?
2(I3/3 C1/1 P4/4 M2/3) = 42 teeth
What is the deciduous dental formula for cats?
2(Di3/3 Dc1/1 Dp 3/2)=26 teeth
What is the dental formula for adult cats?
2(I3/3 C1/1 P 3/2 M 1/1) = 30
Give three examples of micro-organisms.
bacteria
fungi
virus
List the structural organization of the body in order
Cell
tissue
organs
organ systems
organism
What does stress do to the immune system?
Compromises it
What is the generic and brand name of the gas used for gas sterilization?
Generic: Ethylene oxide

Brand name: Anprolene
Which objective is is used with immersion oil and what is the final magnification?
100x objective is the objective oil is used with

final magnification is: 1000x
(Ocular 10x times Objective 1000x)
With an ECG, where to the following leads go:
Red
Black
White
Green
Black
brown
Black -left axillary
Red- left inguinal
(smoke before fire...)

White-Right axillary
Green-Right inguinal
(snow on the grass...)

Brown-chest
What anticoagulant is in the purple top tube-LTT?

What is it used for?

Sample management:
EDTA

CBC

Mix while putting blood in, make 2 blood films right away
What anticoagulant is in the Green top?

What is it used for?

Sample management?
Heprin

Blood gas analysis

mix thoroughly while putting blood in
What anticoagulant is in Red top tube (RTT)?

What is it used for?

Sample management:
NO anticoagulant

bio-chemistries

Allow to clot, spin, separate serum off top-put in separate tube
What tube can be used for a lab test that requires serum?
RTT or Red and gray topped Serum separator tube
What is in a Grey Top tube?

What is it used for?

Sample management:
Oxylate anticoagulant

Glucose measurement

Mix well while putting blood in
What is in a Blue Top tube?

What is it used for?

Sample management:
Sodium citrate anticoagulant

Coagulation Studies

Must be full to vacuum capacity, mix well, spin at 6000rpm for 6 mins, Separate off citrated plasma and put in RTT labled with Pt,s name and that it is Citrated palsma
-<6hrs can be refrigerated
->6hrs-Freeze the plasma
What kind of anticoagulant is in a Blue ring crit tube?

What is it used for?
NONE

PCV, TP, Plasma eval in conjunction WITH a LTT
What kind of anticoagulant is in a Red Ring Crit tube?
Contains HEPARIN

PCV, TP, Plasma eval. when blood is taken DIRECTLY from Pt.
**no syringe involved**
What kind of anticoagulant is in a Black Ring Crit tube?
HEPARIN

used for CBC and blood tests with Avian/Reptile/Pocket pets
What is another abbreviation that means the same as HCT?
PCV

Hematocrit/Packed cell volume)
What size are needles and catheters with a blue cap?

What animals are these typically used on for IM, SQ,IV injections, Cephalic blood draws?
22g

Dogs and cats
Blood draw-jugular of cat, cat size dog
Cephalic of Med to large size dogs
What size are needles with a red cap and who are these used for?
25g

Neonates
Lateral saphenous in dogs
Femoral blood draw in cats
What size are needles with a pink cap?
Who and what are they used for?
20g

IM & SQ-Cows, horses, very large dogs

IV injections-cows and horses

Blood draw-jugular large animal and jugular of med to large size dog
What size needle is the one with a green cap? Who is it used for?
18g

Cows and horses for IM and SQ, jugular blood draw
Give the formula for corrected WBC
WBC * 100
___________________
# of nucleated RBC +100

{(WBC*100)divided by(#nRBC+ 100)}
When is it necessary to do a corrected WBC?
When there are 5 or more nucleated RBC in the WBC count.
What is the name used to describe reticulocytes on a blood film stained with Wrights stain or Diff Quik?
Polychromatophils
What is the name used to describe reticulocytes on a blood film stained with NMB?
Reticulocytes, Retics
What are the 2 types of Retics and how do they differ?
Punctate, more mature with polkadots(ribosomes)

Aggregate, less mature, clumped ribosomes -Only these are couted in cats and birds.
What species is a retic count never done and why?
horses

Because they do not release polychromataphils
What is the purpose of a retic count?
To determine the bone marrows response to anemia
How many WBCs are counted for a WBC diff?
100 cells on 40x
How many RBC's are counted for a retic count?
1000 RBCs are counted and Retics are tallied
Give the order and times of the Gram stain reagents.
1. CRYSTAL VIOLET-for 1 minute

2. GRAM'S IODINE- 1 minute

3. DECOLORIZER- till it runs clear

4. SAFRANIN- 1 minute

*rinse gently with tap water between steps
What color do Gram positive bacteria stain?
PURPLE
What color do Gram negative bacteria stain?
PINK
Name 2 species that are very susceptible to Tetanus and should be immunized with the Tetanus toxid?
Humans

Horses
E.coli is a Gram _____ rod that commonly causes cystitis.
negative
Psittacosis is caused by __________
Chlamydophilia
The vector that transmits the plague is the __________
flea
The Coggins test is used to diagnose_______________
Equine Infectious Anemia (EIA)
Staphyloccus is a Gram positive cocci that is catalase _________
POSITIVE
STREPTOcoccus is a Gram positive cocci that is catalase __________
NEGATIVE
What is used on Gram Positive Cocci for the Catalase test?
H2O2 -hydrogen peroxide
A positive catalase test will do what indicating that it is a ____________?
Fizz and bubble

Gram positive cocci-Staphylococcus
A negative catalase test will do what indicating that it is a __________?
Nothing

STREPTOcoccus
A coagulase test is done on which bacteria?
Gram positive cocci that have tested positive on the coagulase test--indicating Staphylococcus.
A positive coagulase test will have what results and this will indicate what?
The plasma will CLOT

Indicates that it is a PATHOGENIC Staphylococcus such as:
Staph. aureus
Staph. intermedius
Staph. hyicus
A negative coagulase test will have what results and this will indicate what?
no change

Indicates nonpathogenic Staphylococcus such as:
Staph. epidermis
Staph coag- spp
Micrococcus
A negative catalase test indicates that it is STREPTOCOCCUS. What is the next test done to ID the bacteria?
Hemolysis on Blood agar.

Certain bacteria cause lysis of RBCs in Blood agar and sheep blood is often used due its sensitivity to this
What is Beta hemolysis and what bacteria are indicated by this type of hemolysis?
Complete lysis of RBC's in blood agar

Indicates PATHOGENIC STREPTOcoccus such as:
Strep. pyogenes Group A
Strep. agalactiae Group B
Strep. equi Group C
Strep zooepidemicus
Strep. canis
What is Alpha hemolysis and what type of bacteria are indicated by this type of hemolysis?
Incomplete hemolysis of RBCs in blood agar.

Strep. pneumoniae
Strep. suis
Strep bovis
Strep equinus
What is gamma hemolysis and what type of bacteria are indicated by this test?
NO hemolysis on Blood agar

Strep. faecalis (enterococcus
Strep. uberis

*
Bordetella is a GNROD that causes ______________
Infectious tracheobronchitis

AKA: Kennel cough
The gastrocnemius is found on which limbs?
Hind
Tendons connect what to what?
Muscle to bone
Ligaments connect what to what?
Bone to bone
MaConkeys agar selects for the growth of ________________ and differentiate between ___________________ and ___________________.
GNRODs

Lactose fermentors

Non-lactose fermentors
What quadrant is the stomach located in?
Left upper (LU)
What quadrant is the liver located in?
Right and left upper (RU and LU)
What quadrant is the spleen located in?
Left upper (LU)
At what age do puppies normally receive their first Rabies Vaccination?
16 weeks
At what age do we commonly vaccinate puppies?
Starting at 6-8 weeks

Boosters at 12 and 16 weeks
Where do we give the combo vaccine in cats and dogs?
SQ on right front leg, distal to mid-scapula
Where do we give the Rabies vaccine?
SQ on right hind limb, distal to hip
Where do we give the FeLV vaccine?
SQ on left hind distal to hip
Define PO
by mouth
Define CRF
Chronic Renal Failure
Define PRN
As needed
Define PU/PD
Polyuric/Polydipsic
Define IVDD
Intervertebral Disk Disease
Define AIHA
Auto Immune Hemolytic Anemia
Define ACL
Anterior Cruciate Ligament
Define THR
Total hip replacement
Define PCV
Packed cell volume
Define CRT
Capillary Refill Time
Define hypercapnia
Excess CO2 in the blood
Define nystagmus
Rhythmic involuntary movement of both eyes in unison. Movements can be vertical, horizontal or rotational.
Define anisocoria
pupils of unequal size
Define laparotomy
incision through abdominal wall
Define orchiectomy
excision of one or both testes
Define onychecyomy
Declaw, excision of claw, nail, and or nailbed
Define Cynosis
bluish coloring of Mucous membranes due to lack of O2 perfusion to tissues
Define Ataxia
failure to move in a coordinated manner
Define gastropexy
surgical fixation of the stomach to the abdominal wall
Define pyometra
Infection of the uterus with purulent material build up.

Closed- not draining
Open- draining through the cervix and vulva
Define pruritis
Itching
Define encephalomyelitis
Inflammation of the brain and spinal cord
Define NPO
nothing by mouth
Define DKA
Diabetic Ketoacidosis
Define OD
right eye
Define OS
left eye
Define AD
Right ear
Define AS
Left ear
Define CHF
Congestive heart failure
Define MCT
Mast cell tumor
Define IMHA
Immune Mediated Hemolytic Anemia
Define TPLO
Tibial Plateau Leveling Osteotomy
Define GDV
Gastric Dilatation Volvulus
Define PVC
premature ventricular contraction
Define PU (a cat surgery....)
Perineal Urethrostomy
Give the procedure for staining a slide with Diff Quick.
Jar 1 Fixative-5 1 sec dips
Jar 2 Eosinophillic-5 1 sec dips
Jar 3 Basophillic - 7-to 10 1 sec dips

*tap slide on towel between to remove excess
*rinse gently with tap water to remove excess
*Sit upright to remove excess
What is the normal WBC range for dogs?
6,000 to 17,000 / ul (6-17 k/ul)
What is the normal WBC range for cats?
5,500 to 19,5000/ul (5.5 to 19.5 k/ul)
What is the normal RBC count for dogs?
5-10* (10 to the 6th power)
What is the normal RBC count for CATS?
5-11*(10 to the 6th power)
What is the normal PCV for dogs?
37- 55%

Average is 45%
What is the normal PCV for cats?
30-45%

Average is 35%
What is the normal MCV (Mean Corpuscular Volume) for dogs?
60 to 70fl

Above 70flMacrocytic
less than 60fl Microcytic
What is the normal MCV (Mean Corpuscular Volume) for cats?
39-55 fl

Above 55fl -Macrocytic
below 35fl -Microcytic
What is the normal MCHC (Mean Cell Hemoglobin Concentration) for both dogs and cats?
30-36 g/dl

Above 36 g/dl is Hyperchromic
below 30 g/dl is hypochromic
What is the normal range for TP in dogs?
5.0 to 7.0 g/dl
What is the normal range for TP in cats?
5.0 to 8.0 g/dl
What is the normal range for Platelets/ul in dogs and cats?
200,000 to 500,000 platelets/ul
What is the normal blood volume in dogs?
88 mls/kg
What is the normal blood volume in cats?
66 ml/kg
What is the formula for figuring normal blood volume?
Animals weight in Kgs times species norm
What is the fluid replacement formula?
(Weight in #s)*(%dehydrated)*454= mls of fluid needed

OR you can use kgs-

Kgs* % dehydrated*1000=mls needed
How do you arrive at the Observed Retic %?
Move the decimal over to left by one.

For ex- 35 retics becomes 3.5%
6 retics becomes 0.6 %
What is the formula for figuring the Corrected Retic %?
PCV of Pt
______________ * Observed Retic
PCV Avg of Species

---> PCV of Pt divided by PCV average of species TIMES Observed Retic Percentage
What is the formula for figuring Absolute Retics?
# of retics
____________ * RBC(w/o exponent)
1000

=Retics/ul

If RBC is 3.1 *(10 to 6th) you would use 3,100,000 in above formula
What is the formula for MCV?
Mean corpuscular volume is figured by:

PCV * 10
________ = fl (femtoliters)
RBC
What is the formula for MCHC?
Mean Cell Hemoglobin content is figured by:

Hgb*100
------------- = g/dl
PCV
What is the formula for MCH?
Mean Cell Hemoglobin is figured by:

Hgb * 10
-------------- = pg (picograms)
RBC
What is the formula for figuring Fibrinogen?
TP less the hp (heat precipitate)
=g/dl *1000 equals ___mg/dl
What is the formula for figuring TP:F ratio?
Total protein
------------------
Fibrinogen (g/dl answer)

*no units
>15 dehydrated or normal
10-15 gray area
<10 absolute increase in Fibrinogen due to inflammation
What is the medical term for abnormally shaped RBC?
Poikilocytosis
Term for small RBC's with no central pallor?
Spherocytes
Term for nucleated RBC (nRBC)?
Polychromatophil in Diff Quik/ Wrights

Retic in NMB stain
Term for RBC with long, irregular projections:
Acanthocytes
Term for RBC fragments?
Schistocytes
Term for RBC that is spiculated with EVEN small projections over the entire cell surface appearing at times as darker red staining areas.
Echinocytes
Term for RBC that have one or two long spikes coming off of cell that result from a blister or vacuole on surface on cell. Look similar to elmer Fudd hat or half moon...
Keratocytes
Term for an RBC with more surface area than contents. Similar to a half full zip-lock bag. Cell membrane folds and will be lighter in color.
Leptocyte
Term for RBC that has a pale colored mouth area in the center of the cell and is only seen in dogs with chondrodystrophy.
Stomatocyte
Term for a punched out cell. It appears there is a hole in the cell due to very little central pallor with a thickened rim. (red life saver...)
Torocyte
Term for a blister cell that is commonly seen in conjunction with Heinz bodies. Rarely seen
Eccentrocyte
Term for tear drop shaped RBC and is usually artifact, espcially if tear points all point in the same direction.
Dacrocyte
Term for empty RBC membrane and usually indicates IVH.
Ghost cell
Term for residual RNA appearing as very small multiple round spots that stain bluish on routinely stained blood films.
Basophilic Stippling
Term for a Single, sometimes double, inclusion on a RBC seen on a routinely stained film blood film. These dots are basophilic nuclear remnants.
Howell-Jolly bodies
Term for a clear nipple like protrusion from cell wall with routine stain. With NMB stain it will appear as a blue-green protrusion. Can be single or double.
Heinz bodies
What are Heinz bodies caused by and what they result from?
Caused by oxidized and denatured hemoglobin that can result from:
-Oxidant drugs or chemicals like acetominophin, maple leaves and zinc.
-Associated with IV hemolysis
Seen frequently in sick stressed cats
What is the common name of the infection caused by dermatophyte molds?
Ringworm
Dermatophyte test media turns what color______ if positive for Ringworm? What color will the actual growth ON the media be?
Red

White/light colored
What does MRSA stand for?
Methicillian Restistant Staph Aureus
Which is larger in diameter-a 16g or an 18g needle?
16g

-smaller the #, bigger the needle diameter
Which is larger in diameter- a 12fr red rubber cath or a 10fr?
12 french is larger

With Caths-the larger the #, the larger the cath diameter
Is 3-0 suture bigger or smaller than 2-0?

Is size 1 suture bigger or smaller than 4?
3-0 is smaller than 2-0

smaller

smallest ----------------------------largest
<-------------------0-------------------------->
(3-0)(2-0)(1-0) 0 1 2 3 4 5
Autoclaved packs are good for how long?
30 days
Gas sterilized packs are good for how long?
90 days
What size clipper blade should be used for shave a small animal Pt prior to surgical procedure?
#40
A scalpel blade should be passed in what manner?
Overhand with handle to surgeon
Name 5 types of absorbable suture
Vicryl
PDS
Dexon
Maxon
**note these have <6 letters**
Surgical Gut
Name 5 types of NONabsorbable suture
Ethilon (Dermalon)
Prolene
Surgilene/Surgipro
Vetcassette
Novafil
Silk
Steel
Name the tasks the Circulator is responsible for?
Wears only a cap, mask & shoe covers
Drape the back up table
Scrub the Pt
Tie in the surgeon
Plug in cautery
Run anesthesia
Anticipates needs of surgical team
Opens only outer later of packs
Counts USED sponges
Identify this yeast.

Peanut men, bowling pins....)
Malassezia is commonly found in the ear canal, interdigital area (between the toes), anal sacs, vagina, and rectum of healthy dogs. Disease-causing infections with this yeast can occur in dogs of all ages and breeds. There are however, several dog breeds that appear to have an increased susceptibility to developing Malassezia infections including:

* Basset Hounds
* Cocker Spaniels
* English Setters
* Shih Tzus
* Maltese
* West Highland White Terriers
* German Shepherd Dogs
---an opportunistic organism
Name the tasks the Scrub nurse is responsible for
Wears cap, mask, shoe covers, sterile gown and gloves
Gown and glove the surgeon
Load the needle drivers
Drapes the mayo stand
Drapes the Pt
Arranges instruments on Mayo stand
Counts UNused sponges
uses a closed gloving technique
With a refractometer, what is read on the right hand side scale?

Left hand scale is for?

Middle scale?
Urine Specific gravity (USG), no units
1.018 would be siad out load as ten-eighteen.

Total Protein (TP), g/dl

Nothing we care about
What is the lab procedure for preparing PCV tube?
-Obtain EDTA blood or several heparinized HCT tubes. Mix well
-Fill at least 2 plain(non-hepranized) HCT tubes 2/3 to 3/4 full
-Plug ends with crit clay
-Spin for 5 minutes at 6000RPM
What is the anatomy of a spun crit tube?
Plasma, buffy coat, RBC's
What does PCV measure?
The percent quantity of blood which is made up of RBCs
How do you measure the PCV?
Using a PCV/Hematocrit card, -bottom of the Red Cell Column is placed at 0%
-top of the plasma is placed where it hits 100%.
-Read PCV where the Red Cell column top is at.
What is the procedure for figuring TP?
Take the previously used PCV tube after reading PCV % and break it just above the buffy coat.
-Put the plasma on the glass pane of the refractometer, close and look through eyepiece
-Read left hand scale to the nearest 0.2 g/dl
What can be seen in the buffy coat if it is examined under a microscope on 10x?
Circulating microfilaria
ID this dermatophyte mold. What does it cause?
Microsporum canis

Ringworm
ID general type of bacteria
GNROD
ID general type of bacteria
GNROD
What crit tube is used for WBC count blood film?
Blue ring with no anticogulant in it in conjuction with LTT
What is the technique for making a blood film?
-Use fresh whole EDTA well mixed blood
-Using blue ring crit tube, place small drop on end of clean glass slide
-Using coverslip or spreader slide, draw the slide backwards through drop so blood collects along the edge.
-With Coverslip or spreader slide at a 30 degree angle, move it along the slide in an even stroke along the slide
-wave in air to dry
-Make sure there is a good feather, monolayer and body
-Stain with Diff Quik, or Wrights
What is ID'd on 10x in the feather?
-WBC distrubution
-Platelet clumping (too much may invalidate platelet count-REDO film)*
-Abnormal cells*
-Microfilaria*
*items to be quantified
What is ID'd on 10x in the Monolayer of the blood film?
Is it of Adequate size?
WBC distribution
What is ID'd and checked in the body of the blood film?
-RBC associations
-Rouleaux-stacked pancakes, chains*
-Agglutination-clusters and blank spots of RBC's **Never normal
What test is used to differentiate if an RBC association is Rouleaux or agglutination?
Saline Wash-
Rouleuaux will wash out, agglutination sticks together like glue
What species is Rouleaux a normal finding?
horses
Where do we examine the blood film for WBC?
Monolyer on 40x

Begin dif and morphology assessment
Where and at what objective do we use for RBC & WBC morphology, platelet assessment?
Monolayer

100x oil objective
What RBC morphology is assessed on 100x?
Size,
shapes,
color
+/- inclusions
ALL are quantified as mild, moderate, marked
What type of WBC morphology is assessed on 100x?
nuclear assessment
cytoplasmic condition

ALL are quantified as mild, moderate, marked
What is assessed for Platelets on 100x?
-Macro or mega size
-Shape
-Membrane reactivity (hairy=reactive)
-Estimate 10 fields
-8-30 pltlts appear adequate
-< 8 appear decreased
>30 appear increased
What is the flow of blood through the heart and body?
1. Vena cava-
2. Right atrium
3. Tricuspid valve
4. Right ventricle
5. Pulmonary valve
6. Pulmonary artery
7. Lungs
8. Back to heart via the pulmonary vein
9. Left atrium
10. Bicuspid valve
11. Left ventricle
12. Aortic valve
13. Aorta
14. Organs in the body
ID and what does it cuase?
Coccidia are small protozoans (one-celled organisms) that live in the intestinal tracts of dogs and cats. They cause disease most commonly in puppies and kittens less than six months of age, in adult animals whose immune system is suppressed, or in animals who are stressed in other ways (e.g.; change in ownership, other disease present).

In dogs and cats, most coccidia are of the genus called Isospora. Isospora canis and I. ohioensis are the species most often encountered in dogs. Regardless of which species is present, we generally refer to the disease as coccidiosis. As a puppy ages, he tends to develop a natural immunity to the effects of coccidia.
What is FIV and what causes it?
Feline Immunodeficiency Virus
--family Retroviridae, Genus Lentevirus

Because biting is the most efficient means of viral transmission, free-roaming, aggressive male cats are the most frequently infected.
-Infected cats may appear normal for years. However, infection eventually leads to a state of immune deficiency that hinders the cat's ability to protect itself against other infections. The same bacteria, viruses, protozoa, and fungi that may be found in the everyday environment--where they usually do not affect healthy animals--can cause severe illness in those with weakened immune systems. These secondary infections are responsible for many of the diseases associated with FIV.
ID and what does this cause?
Otodectes ear mite

ear mites move about and feed along the cat or dog's ear canal they create substantial irritation and subsequent inflammation. The mites feed on tissue debris and secretions from the ear canal lining. With repeated irritation, the ear canal thickens and debris builds up within the ear canal. This debris, caused by mite waste products, dead tissue and fluid resembles coffee grounds. In chronic (long term) cases of ear mites in cats and dogs, there often is secondary bacterial and yeast infections present that complicate the treatment and create even more stress on the animal.
What is the normal gestation of dogs and cats?
63 days
What type of sling is used to immobilize a HIND limb AFTER coxofemoral dislocation?
Ehmer sling/aka Figure 8 sling

-rotates hip inwards so head of femur will sit in acetabulum
What type of splint is used for non-displaced fracture typically to help stabilize it?
Schroeder Thomas Splint

-frame made of aluminum, sits on hip, limb suspended after wrapping within frame.
What type of sling is used to prevent weight bearing on front limbs such as for shoulder dislocations and injuries?
Velpeau sling-holds flexed limb against chest
What is the bandage application procedure?
-clean wound
-Apply Telfa pad/non-adherent dressing
-Apply tape stirrups if needed-
-Apply cotton padding/cast padding
-wrap with gauze
-Fold stirrups up if used
-wrap with vet wrap, elasticon, tape
-date bandage with a permanent marker
When wrapping a bandage, what direction is it done?
Distal to proximal
-helps blood flow back to the heart and prevents swelling
If you are wrapping a long bone fracture, what is one important thing that should be done to immobilize it?
Wrap above and below the joints proximal and distal to fracture
Why are at least 2 toes left exposed when wrapping a limb?
To check for swelling, temp, swelling, bandage tightness
When is a Modified Robert Jones bandage used?
-Most common lower limb bandage
-Used for light support to reduce swelling due to Sx and to cover wounds that require protection.
What is a Robert Jones bandage?
-A short term bandage
-Provides temporary support/stabilization of Fx/dislocations distal to elbow or stifle
-Generally used Pre-op
--VERY bulky
_Animal typically stays in hospital due to size of bandage
ID instrument and its use
Periodontal Probe- hand instrument w/indentations spaced in millimeters for measuring the depth of the gingival sulcus around the tooth
Norms: Dogs 1-3 mm, cats 0-1mm
ID this instrument and it use
Hand scaler-instrument with a triangular blade that curves and tapers to a point on end.
-Used to remove tartar/calculus SUPRAgingavally
Id this instrument and its use
Dental Curette-has 2 sharp sides and a rounded tip
-Used to remove SUBgingival calculus
Id this instrument and its use
Dental hoe-

Used for removal of large deposits of calculus on the supragingival surface of the tooth
ID this intrument and its uses
Dental elevator-spoon like tip with sharp edges on a large handle
-Used to break the periodontal ligaments BEFORE extraction of a tooth.
ID this instrument and its uses
Extraction forceps
-used to grip the tooth to lift it out for extraction after periodontal ligaments have been broken down
-Can be used to remove very large chunks of tartar
Name the 5 lymph nodes sites in a dog
Axillary
inguinal,
prescapular
popliteal
submandibular
Lymph node locations
image
Define CVT
Certified Veterinary Technician

A Certified Veterinary Technician's duties include taking medical histories, treating routine problems, and counseling pet owners. Technical duties involve:

* taking blood, urine, skin and other samples
* performing lab procedures
* administering anaesthesia and other medications
* taking x-rays, ultrasounds, and sonographs
* Assisting veterinarians in surgery
* maintaining and servicing veterinary equipment
* keeping treatment records and supply inventories
Define enucleation
Sx removal of the eyeball
Define idiopathic
Disease or condition of unknown origin
Define mydriasis
Dilation of the pupils
Define miosis
constriction of the pupil
What is the medical term for incision into the bladder?
Cystotomy
What is the medical term for heartworm and what is responsible for transmitting?
Dirofilaria immitus

mosquito
ID this cath
Foley cath- a longer term indwelling cath
ID this cath
Red Rubber cath that can be indwelling.

Also used as O2 NG tube and a feeding tube
Id this cath
Steel bitch cath used only on female dogs
What are polypropylene caths used for?
A rigid plastic cath for very short term use such as obtaining a urinary sample
Who do cows typically get rabies from?
Bats and sometimes skunks
What are the 2 general types of test for parasites?
i. Qualitative “find the parasite” more common
1. Simply-does the animal have the parasite at that time
ii. Quantitative- “how many does the animal have?”
1. Approximate degree of infestation
2. Never do unless there is a positive qualitative test
3. Seen more often in research
What are the 3 sample types for parasite quantitative tests?
Fecal
Urine
blood
What are the 3 QUALITATIVE tests done most often on fecal samples?
Gross visual Exam
Direct Smear
Concentration or float technique
If you are doing a fecal and you see no ova or parasites, what do you write in the record and lab log?
NSATT-nothing significant seen at this time

OR-
NoPSATT-no parasites seen at this time
What is the fecal 3?
Direct exam
Float
Cytology
What are the 3 main purposes in veterinary parasitology?
Locate
ID
Treat
What is the rule about objectives in parasitology?
LOCATE on 10x, ID on 40x

Can see tapeworm segments, fleas, ticks, mites on 4x

Giardia cysts & trophazoites are located and ID'd on 40x
What is looked for on a Gross Visual exam?
a. Tapeworms often diagnosed this way
b. Sometimes adult worms are passed this way
c. Odor can give hints to –foul smelling may = Giardia or Parvo puppy
d. Consistency-may hint towards Giardia or viral bacterial disease
e. Mucus may be indicative of colitis
i. Whipworms are known to cause excessive mucus in sample
A DIRECT SMEAR is always done.
Give the basic procedure of how this is done.
i. Tiny fleck of well mixed sample
ii. 2-3gtts of PSS
iii. Place fleck on slide, add PSS. Take cover slip and with even pressure, skim sample across, Drop slip on sample.
iv. Place slide on stage-condenser down, locate on 10x, ID 40x
v. ONLY TEST YOU CAN SEE LARVAE ON
When doing a a microscopic evaluation in parasitology, where should you condenser be positioned ?and why?
DOWN

Too much light will make it difficult or impossible to see the ova, larvae---washes out the sample making diagnosis difficult.
What is the specific gravity of flotation solution?
1.180-1.200

This leads to heavier material sinking and OVA floating on top & sticking to coverslip. LARVAE will SINK--so will NOT be seen
How long do fecal float cover slips typically sit on the meniscus of float?
10 minutes, it is then placed on slide for examination.

*Giardia trophozoites usually rupture in these solutions, so will not be seen.
IHow much feces is typically used with a fecal float? Why is this an advantage?
About the size of a pea, so larger than a direct smear, giving a better chance of seeing something.
What are 3 types of Floatation media?
Sodium nitrate/NaNO3-Fecasol, Flomed, Ovassay

Zinc Sulfate/ZnSO4-best type but more expensive

Sheather's suger-specific gravity and will float ANY ova
Once your fecal float cover slip is placed on a slide, what objectives are used?
LOCATE on 10x, ID on 40 x
What can be ID'd on a Fecal float slide?
Parasite ova, Coccidia and possibly Giardia with Zinc Sulfate
What are the advantages of using the Centrifugation for floatation/concentration exam?
i. More efficiently recovers eggs and cysts
ii. Requires less time
iii. Less egg/cyst distortion, less rupture potential
iv. Even less fecal debris than all other techniques
What type of centrifuge is used for fecals?
A swinging head centrifuge

*must be balanced with even number of Test tubes
How long are samples for parasitolgy fecals spun for?
1200-1500 for 3 minutes
What is the procedure for doing a FECAL CYTOLOGY/FECAL SMEAR?
1. Obtain a FLECK of feces
2. Roll sample onto slide-THIN layer, allow to dry or heat fix
3. Stain with Dif-Quik
4. Examine on 100x oil immersion
What is the purpose of a fecal cytology?
Only method that can be used to ID CLOSTRIDIUM-looks like spores, safety pins, tennis rackets
and
CAMPYLOBACTER- looks like seagulls, spirochetes
What is is the Dif Quik staining procedure?
1. Jar 1-Fixative (bluish-green) Do 5 1 second dips
2. Jar 2-Eosinophilic (Red) Do 5 1 second dips
3. Jar 3-Basophilic (Purplish) 7-10 1 second dips
4. Rinse with sink water and allow to dry
What is the procedure for reporting out findings on a fecal cytology/
i. Normal to see <5/hpf
ii. Always report out # per hpf
iii. Check a minimum of 10 fields and average
ID
Giardia cysts and trophozoites
ID
Giardia cyst
ID
Giardia trophozoites
ID
Campylobacter bacteria
ID
Campylobacter bacteria
ID
Clostridium perfringes
What are the major categories of neurotransmitters?
Amino acids (NH2) (glutamate, GABA, glycine, aspartate)
Acetylcholine (a mModified amino acid)
Monoamines (indoleamines, eg serotonin; catecholamines, eg dopamine, norepinephrine, epinephrine)
Peptides (endorphins, substance P, neuropeptide Y)
Purines (ATP, adenosine)
Gases (Nitric oxide)
ID
Coccidia unsporulated

*can't tell which one it will be till it sporulates
ID
Coccidia isospora
ID
Coccidia isospora
What are the 2 major activities for daily living (ADL) assessment guidelines for the Young Adult
(Healthy Lifestyle)
1. Exercise
2. Diet
ID
Coccidia eimeria (large animals only)
What is DIC?
Disseminated Intravascular Coagulation
-Secondary to some other disease
-Is an excessive activation of clotting factors
-Clots flow throughout the microcirculation-->hypoxia--> ischemia-->organ dysfunction-->Clotting factors leads to body reversing and no longer clotting---->Fibrinolysis is activated and excessive FDP's break down clots and keep others from forming---->animal bleeds out.........

Rapid process that is VERY difficult to reverse. May give heprin to reverse process early on but low chance of success.
Define gingiva
periodontal MM covering bone of maxilla and mandible
define gingival sulcus
groove formed between neck of tooth and free edge of gingiva.
Where is the neck of the tooth?
found at hte gingival margin where crown and root meet
Where is enamel?
covers the crown of each tooth, hardest substance in the body.
Where is cementum?
substance covering the root of a tooth.
Where is dentin located?
a porous bony substance located beneath enamel and cementum of tooth
Where is the pulp cavity located?
A canal located in the center of dentin that is filled with soft tissue, blood vessels and nerves.
Review
diagram review
Define Apical delta
where blood vessels and nerves enter and exit the tooth
What is the purpose of periodontal ligaments?
connective tissue that holds tissue to bone
What is the buccal surface of a tooth?
comes in contact with CHEEKS
What is the labial surface of a tooth?
Comes in contact with lips (incisors)
What is the lingual surface of a tooth?
comes in contact with the tongue (mandibular teeth only)
What is the palatal surface of a tooth?
Comes in contact with palate (maxillary teeth only)
What is the mesial surface of a tooth?
Side of tooth that comes in contact with mid-line, closest to front of mouth, incisors
What is the distal surface of a tooth?
Side of tooth farthest away from mid-line, incisiors
What is the occlusal surface of a tooth?
surface of the tooth that comes in contact with opposite tooth on opposite jaw. the BITE surface
In the dog, which tooth is the upper carnassial tooth?

How many roots does it have?
4th premolar, #108 and 208

3 roots
By what age are most permanent teeth in?
5-6 months
By what age do most puppies and kittens eyes open?
7-14 days
What is the common name for the Trichuris vulpis?
Whipworm
What is the common name for the Ancylostoma caninum?
Canine hookworm
What is the common name for Toxocara canis?
Canine roundworm
What is the common name for Dirofilaria immitus?
Heartworm
What is the common name for Dipylidium caninum?
Double Poured tapeworm
What is the common name for Paragonimus kellicoti?
Lung fluke
What is the family name for the Isospera/Eimeria?
Coccidia
What is the common name for the Strongylus vulgaris?
Blood worm, Large strongyle
What is the common name for the Oxyuris equi?
Pinworm
What is the common name for Fasicola hepatica?
Common liver fluke
What is the common name for Rhipicephalus sanguineus?
Brown Dog tick
What is the common name for Cutebra?
Rabbit bot
Pregnant women should stay away from cleaning litterboxes due to possible transmission of which parasite?
Toxoplasmosis gondi
VLM in people is caused by which parasite?
Roundworms
CLM in people is caused which parasite?
Hookworms
What is the genus and species of the most common cause of ringworm in dogs and cats?
Microsporum canis, a mold
What lab animals require supplementary vitamin C?
Guinea pigs and NH primates
What are the common names for the 3 most distal joints in the equine front leg from distal to proximal?
Coffin joint
Pastern joint
Fetlock joint
Give the common name for the distal phalynx in a horse.
Coffin bones
In a dog and cat, what bones are immediately distal to the carpus?
Metacarpals
For a small animal bandage, list the order of bandaging material for a basic bandage.
Primary layer dressing-Telfa pad
Cotton and or Cast padding
Kling or conforming gauze
Vet wrap/Elastikon
What type of fractures is a Robert jones bandage used for?
To stabilize fractures distal to elbow or stifle
What is an Ehmer sling used for?
To immobilize the hindlimb following a reduction of a luxated hip
What is responsible for causing walking dandruff?
Cheyletiellosis, a surface mite
What is the common name for the yeast that causes otitis in dogs?
Malassezia
What is the name of the tick that is a vector for Lyme disease?
Ixodes tick
Demodex is a burrowing mite that is diagnosed with what type of procedure?
Deep skin scraping
ELISA heartworm tests detect what types of antigens?
Adult female heartworm
What is the genus name of the common ear mite?
Otodectes
FelV and FIV are in what virus family?
Retroviradae
What family is FIP virus in?
Cornaviridae
What family is the Panleukopenia virus in?
Parvoviridae
Define tachycardia
abnormally rapid heartbeat
Define dyspnea
labored or difficulty with breathing
define hyperkalemia
Abnormally high potassium concentration in the blood
Define hyponatremia
deficiency of sodium in the blood
Define sublingual
beneath the tongue
Define anastamosis
surgical procedure to connect 2 previously unconnected tubular organs or parts of the same organ
Define intussusception
Prolapse of one part of the intestine into the lumen of the immediately adjacent part, causing intestinal obstruction
Define entropion
inversion, turning inward, of margin of the eyelid
What is Strangles caused by?
Streptococcus equi
What causes Circling Disease?
Listeria monocytogenes
What causes Lyme disease?
a spirochete-Borrelia burgdorferi

Transmitted by Ixodes tick
What causes Tropical Canine Pancytopenia?
Erlichia canis,

Transmitted by Brown dog tick
What causes tuberculosis?
Mycobacterium spp
What causes Cat Scratch Disease?
Bartonella henselae
What causes Rocky Mountain Spotted /Tick fever?
Rickettsia ricketsii
What are 3 Flatworm Flukes we studied?
Fascicola hepatica
Paragonimus kellicotti
Nanophtes salmincola
What are the Genus of tapeworms we studied?
Dipylidium caninum
Taenia spp.
Echinococcus spp (causes Hydatid cysts!)
Ancoplocephala spp (horses)
Monezia spp (cows)
What are some basic facts about Tapeworms?
All L1 infective except Taenia pisiformis
-Indirect life cycle
-Monecious
-Have hexacanth embryos within ova
-Segment is a proglottid
-A strobila is a chain of reproductive units
What are some basic facts about Roundworms/Ascarids?
-Direct life cycle
-Dioecious
-Migration needed to complete life cycle -Tracheal, Somatic, Muccosal
What are the roundworms/Ascarids with a Ropey OUTER shell with a large dark nucleus?
Toxocara spp
Which are the roundworms with SMOOTH Ropey INNER shell and a lighter smaller nucleus?
Toxascaris leonina
What are some basic facts about Hookworms?
-BLOOD FEEDERS!
-Reported as hookworms in small animals, TASE in large animals
What are the basic groups of hookworms discussed in parasitology?
Ancylostoma spp
Uncinaria stephala
Bonostomun spp.
What are some basic facts about Lungworms and what are some groups?
-Type of roundworm

-Aleurostrongylus abstrusus (cat)
-Dictyocaulus spp (cattle)
Metastrongylus apri (swine)
Eucolus (Capillaria) aerophila
What are some basic facts about Ruminant TASE?
-Reproductively aggressive
-Premunition is the buildup of resistance
-L4 is the hypobiotic stage-arrested development until time (season) is right to continue
What are the parasites that make up the HOCT complex of Ruminent TASE?
Haemonchus contortus
Ostertagia spp(imp parasite in young cattle)
Cooperia spp ( Cattel Bankrupt worm)
Trichostrongylus spp
and Nemotodirus spp, which is not a TASE
Which is more pathogenic, large or small strongyles?
LARGE
What is a facultative parasite?
When only the female is pathogenic
What are 2 types of equine TASE?
Strongyloide westeri -small

Strongyloide vulgaris - large
What is the Genus & species of the Esophageal worm?
Spirocerca lupi
What is the Genus and species of the Giant kidney worm?
Dioctophyma renale
What is the genus and species of the Bladderworm?
Pearsonema (capillaria) plica and cati
What is the genus of the whipworm?
Trichuris spp.
-double operculated ova
What is the genus and species for the parasite that causes Heartworm and what is it's IH?
Dirofilaria immitis

Mosquito
What are the Burrowing mites?
-Recovered w/a deep skin scraping for diagnostics
-Demodex spp
Demodectic mange
Follicular Mange
-Sarcoptes Scabei (Mange)
-Notoentendres cati (Feline scabies)
-Knemokoptes spp. (mange)
What are 2 types of Surface mites?
-Recovered with a light skin scraping and a scotch tape prep is used
-Otodectes cynotis (Ear mite)
-Cheyletiella spp
-walking dandruff, have palpebral claws
What are the 2 types of Gram positive cocci?
Staphylococcus
Streptococcus

-Catalase test used to differentiate
Bubbles-positive test, indicates Staph
What test is used to differentiate between Pathogenic and non-pathogenic Staph?
Coagulase test-clot is positive result which means it is Pathogenic
Name some SMALL Gram positive rods
Remember CLEAR!!
-Corneybacterium
-Listeria
-Erysipelothrix
-Arcanobacterium
-Rhodococcus

-More important in large animals
Name some Large Gram positive rods
Bacillus
-aerobic, endospores, produce exotoxins

-Clostridium
-ANAEROBIC, endospores, exotoxins
What are some branching Gram positive Rods?
* these are a higher evolutionary form of bacteria that are fungi like and show filamentation and branching

-Actinomyces
-Nocardia
-Streptpmyces
-Dermatophilus
What are some basic facts about Corneybacterium?
-Club shaped rods
Cause:
C. pseudotuberculosis
-CLA Caseous lymphadenitis (sheep & goats)
-Ulcerative lymphangitis (horses and mules) AKA-Pigeon fever

C. renale -has pili
-causes bovine peyelonephritis, cystitis
-Sheep-causes "pizzle rot" (balanothitis, vulvulitis)
-
What are some basic facts about Listeria monocytogenes?
-Motile bacteria
-Ubiquitous-found everywhere ( water, feces, soil, silage, milk)
-Common cause of food poisoning due to the fact that it replicates at fridge temps.
2 forms-
Visceral-monogastric causes abscesses on internal organs
NEURAL-Causes CIRCLING DISEASE in ruminants, esp cattle and sheep.
-affects CNS, meningitis, Unilateral ataxia
What are some basic facts about Erysipelothrix rhusiopathiae?
-Pathogenic for pigs, birds (turkeys), fish, marine mammals, people
-NF in mouth and throat of swine, fish slime
-Swine form Causes:
-Diamond Skin disease
-Arthritic Form in older pigs, -Acute-causes septicemia, -Cardiac Form
-Causes Fish Handlers disease in people who handle fish
What are some basic facts about Arcanobacterium pyogenes?
-Affects ruminants via a wound
-Causes pyogenic infections of wounds, Sx incisions as well as mastitis, Pneumonia, Umbilical infections
What are some basic facts about Rhodococcus equi?
-Causes a suppurative Bronchopneumonia in foals btwn 1-6 months, will have dyspnea, crackles
What does a positive result on a H2S production test look like?
Agar will have black pigment
What does a positive motility test look like?
Agar will be cloudy throughout media
What are some basic facts about Bacillus?
-Aerobic, form chains and endospores, excrete exotoxins
What are some basic facts about Bacillus anthrasis?
-Large GPROD
-Causes ANTHRAX
-Obiligate pathogen of people and animals-Usually fatal if inhaled
-Can be inhaled or penetrate skin
3 forms:
Cutaneous-spores enter a wound (Woolsorters disease in people)
Pulmonary-inhalation of spores, most deadly-fatal w/in 24 hours
GI-ingestion of spores. Most common form in dogs and cats
-Reportable to the state
What are some basic facts about Clostridium?
-ANAEROBIC, endospores, excrete exotoxins
Species:
-botulinum
-tetani
-perfringes
-septicum
-chauvoei
-novyi
-haemolyticum
What are some basic facts about Clostridium botulinum?
-Causes botulism
-Acquired via ingestion of pre-formed exotoxin in a food source
-Exotoxin is a neurotoxin-one of the most potent biological toxins
-this endotoxin acts on the neuromuscular junction to prevent the release of acetylcholine which results in weakness and paralysis
-Causes weakness in hind legs at first and progresses crainally, eventually paralyzing diaphragm leading to respiratory arrest
What are some basic facts about Clostridium tetani?
Large GPROD, anerobic, exotoxin, endospore that resides in the soil
-Causes Tetanus/Lockjaw
--Humans and horses most susceptible
-Carnivores least susceptible
-Acquired by spore from enviro entering a deep puncture wound, it grows in the anerobic enviro and releases exotoxin
-Exotoxin is a neurotoxin that acts on CNS and blocks the function of inhibitory neurons, which results in inhibitory neurons not being able to tell muscles to release contraction
What are some basic facts about Clostridium perfringes?
-Causes Clostridial enterotoxemia in people, dogs, cats & large animals
-common cause of food poisoning in people
-Can cause would infection, gas gangrene
-Acquired by ingesting spores
-Causes:
-Hemorrhagic enteritis-diarrhea w/blood and mucus
-Systemic toxemia in large animals
-Diagnosed via fecal cytology- will see bacteria that look like SAFETY PINS
What are some basic facts about Clostridium septicum?
-Histotoxic
-Causes Malignant edema (anaerobic myocytis) that causes muscle inflammation
-affects large animals
-Exotoxin causes tissue necrosis, edema, gas gangrene, systemic toxemia **HIGHLY FATAL
What are some basic facts about Clostridium chauvoei?
-Causes Black Leg Disease in large animals via spores being ingested or entering a wound
-Causes swelling, edema, gas gangrene, DISCOLORED skin, systemic toxemia
What are some basic facts about Clostridium novyi?
-Causes Black Disease/Infectious Necrotic hepatitis
-Acquired via ingestion of spores and worse w/pre-existing liver damage
-Exotoxin causes liver necrosis
What are some basic facts about Clostridium haemolyticum?
-Causes Red Water Disease or bacilliary hemoglobinuria in large animals
-Acquired via ingestion of spores which grow in damaged liver
What are some basic facts about Branching GPRODS?
-Less common cause of disease
-Four genuses:
Actinomyces
Nocardia
Dermatophilus
Streptomyces
What are some basic facts about Actinomyces?
-Anaerobic to microaerophilic depending on species
-NF in GI tract & oral cavity of many animals including humans
-Invade previously damaged tissue or penetrate a body cavity
-Highly diagnostic-clumps or masses of branching bacteria
-Will have Sulfur granules that will need to be squashed & stained--then you will see the branching filamentous G+ rods
What are some basic facts about Actinomyces bovis?
-NF in oral cavity of cows
-Causes Lumpy Jaw in cattle
What are some basic facts about Actinomyces viscosus?
*Important cause of periodontal disease in people
-Causes Actinomycosis
-Acquired via spores entering wounds in oral cavity, penetration of body cavity
What are some basic facts about Nocardia asteroides?
AEROBIC branching GPROD, less common
-Causes Nocordiosis
-Will seen in wounds such as fistulas and draining tracts
-Causes pyothorax
-Can cause Mastitis
-Desseminated form spreads to internal organs
What color do Gram negative bacteria stain and what kind of toxins are they associated with?
Pink

ENDOtoxins
What are the 2 types of Gram Negative Rods?

What test is done to differentiate between the 2?
Enteric and Non-enteric

Oxidase test-
A purple/dark spot on filter paper means it is a NONenteric
Name the Enteric Gram negative Rods
-Escherichia coli
-Klebsiella (slimy, snotty colonies)
-Proteus
-Serratia
-Shigella
-Yesenia (pestis causes PLAGUE!)
-Enterobacter
-Citrobacter
Name the NON-enteric Gram Negative Rods
(the -ellas, -monas and a -bacter)
-Brucella
-Pasturella
-Francisella
-Pseudomonas
-Bordetella
-Moraxella
-Campylobacter
What are 2 spirochetes we discussed in microbiology?
Leptospira

Borrelia burgdorferi-causes Lyme disease via vector-Ixodes tick (reservoir is White footed mouse)
What are some basic facts about Escherichia coli?
-aka---E. Coli
-Most encountered bacteria in microlab
-NF in small intestine and Colon
-MANY different strains and serotypes
-2 Catagories
Opportunistic-NF but will cause infections if the get into an area that is supposed to be sterile (bladder, uterus, wounds)

Enteropathogenic-have pili, cause Gastroenteritis in newborns.
What are some basic facts about Enteropathogenic E. Coli?
Pathogenic in 2 ways:
-Invasive-invade tissues
Penetrate intestinal mucosa, causing lesions, enteritis leading to septicemia, endotoxemia

Enterotoxin-releases exotoxin into intestines which binds with and disrupts cell membranes of intestines resulting in those cells losing water, electrolytes, bicarb.
-death due to dehydration and metabolic acidosis
What are some basic facts about Klebsiella pneumoniae?
-Encapsulated bacteria, with a characteristic colony morphology that involves mucoid, slimey, stringy colonies--will "string off of plate"
Causes:
Cervicitis and metritis in mares
Pneumonia in foals
Mastitis in cattle
What are some basic facts about Proteus mirabilis and Proteus vulgaris?
-highly motile, swarms on blood agar
-Swarming colony morphology
-Smells like ammonia
-Causes a variety of infections
UTI, Otitis,Wounds infec., Mastitis
What are some basic facts about Salmonella?
-Many species and serotypes
-Fecal-oral route of transmission
-Causes Salmonellosis from contaminated food or water. 2 forms:
Gastroenteritis-most common, bacteria invade intestinal mucosa and cause diarrhea, fever
Septicemia-enters bloodstream of immunocompromised animals
What are some basic facts about Yersinia pestis?
-Causes plague, which occurs in western 1/3 of US.
-Reservoir host-Rodents, Prairie dogs are sentinels
-Humans and Cats are most susceptible
-Vector is the flea
3 forms:
-Bubonic -most common, enlarged LN's, fever, lethargy
-Septicemic
-Pneumonic
What are some basic facts about Shigella?
-Similar to Salmonella
-Causes Shigellosis-hemorrhagic watery diarrhea
Pathogenic for primates, rare in dogs & cats
What are some basic facts about ID'ing Enteric GNRODs?
Gram stain -PINK
Oxidase negative-no color on filter paper
Grow on MacConkey's Agar
-Lactose fermentors will have PINK growth
-NON-lactose fermentors will have Colorless growth
What do Fusobacterium and Bacteriodes cause?
-Foot rot in large animals
-Fuso-causes thrush in horses 9stinky necrotic tissue)
Broad spectrum antibiotics are effective against?
Gram positive and some Gram negative bacteria
Narrow spectrum antibiotics are effective against?
Either Gram negative or Gram positive bacteria, depending on the type. Will only work on one type
Define bacteriocidal
Antibiotic that KILLS bacteria
Define Bacteriostatic
Antibiotic that inhibits replication of bacteria and then relies on host's immune system to clear up remaining bacteria
What are antibiotics 5 Mechanisms of action (MOA)?
-Inhibit cell wall synthesis (cidal)
-penicillin
-Alter cell membrane permeability (cidal)
-polymixins
-Inhibit protein synthesis (static)
-tetracyclins
-Inhibit nucleic acids
metronidazole/Flagyl
-Interfere with a metabolic pathway (static or cidal)
-sulfonimides
What are some basic facts about Brucella?
-Small non-enteric GNROD
-Aerobic to microaerophilic
-Some species can remain viaible in urine, milk, damp soil, aborted fetus for as long as 4 months
--Intracellular bacteria that survives and multiplies within phagocytes
-Invades tissues of Reproductive tract
What are some basic facts about Brucella canis?
-infects dogs, reportable to state
Causes:
-Abortion at about 45-55 days
-epididymitis/orchitis/protatitis
-testicular atrophy, scrotal dermatitis
-infertility and conception failures
Diagnosed w/serology
Breeding dogs should be tested yearly
-Very difficult to Tx and euthanasia is recommended
What are some basic facts about Brucella abortus?
-Infects cattle and related animals
-Called BANGS disease
-Causes
-undulant fever in humans
-Abortion storms in last trimester
-mastitis
-testicular abnormalities/orchitis
-Infertility, conception failures

-Transmission via :
ingestion of aborted fetus/placenta, urine on grass, milk
-breeding and AI
How is Brucellosis controlled in cattle?
-Immunization of female calves at 4-12 months-ID'd by tattoo in right ear and an orange ear tag
-Serology tests-prior to breeding, shipping, sales. Dairy cows tested annually
What are some basic facts about Brucella suis?
-infects swine via ingestion or breeding
What are some basic facts about Brucella ovis?
-infects sheep, rams more than ewes
-Causes orchitis, epididymitis, infertility
-Males are tested and immunized
What are some basic facts about Brucella melintensis?
-infects cattle, sheep, goats, camels, llamas
What are some basic facts about Bordetella bronchseptica?
-Causes KENNEL COUGH/Infectious tracheobronchitis in dogs
and atrophic rhinitis in piglets
-Have pili that make them resistant to MCTS
-Infection via inhalation
-Destroys ciliated epithelium
-Classic symptom is a DRY hacking non-productive cough
What are some basic facts about Pasteurella?
Non-enteric GNROD
-Antibiotic resistant
_NF in oral cavity & respiratory tract in many species, esp cats
What are some basic facts about Pasteurella haemolytica?
-Now known as Mannheimia haemolytica
-Reservoir is NF of nasopharynx in Cattle
Called: Shipping Fever causing bronchopneumonia
Can affect all cows but calves more susceptible
-3 predisposing factors
-Presence of Pasteurella/Mannhemia bacteria
-Presence of additional virus such as IBR, BVD,
-Stress ( such as shipping)
Tx-antibiotics
Can vaccinate for respiratory viruses
What are some basic facts about Pasteurella multocida?
-NF in oropharynx of many species
-Causes:
Snuffles in Rabbits
Avian cholera in birds
BITE WOUND ABSCESSES
URI, Otitis
Septicemia, meningitis in humans from bite wounds (found in 75% of bite wounds...)
What are some basic facts about Moraxella bovis?
Non-enteric Gram negative coccobaccilus (tiny!)
-NF on conjunctiva & nasopharynx of cattle
Disease is called IBK-Infectious Bovine Keratoconjuctivitis (cattle Pink Eye)
Solar radiation predisposes white faced cattle to higher rate of infection
-flies are a mechanical vector
dry dusty conditions can cause outbreaks
causes conjuntivitis,corneal edema, mucopurulent ocular discharge
-Zoonotic
What are some basic facts about Francisella tularensis?
-Non-enteric GNROD
Reservoir-rabbits, rodents
Disease is called Tularemia/Rabbit fever and can affect humans, dogs, cats
-Transmitted via tick vectors, ingestion of rabbit meat and inhalation of aerosolized blood
-Pathology similar to bubonic plague-enlarged lymph nodes, high fever, fatigue, anorexia
What are some basic facts about Pseudomonas aeruginosa?
-Ubiquitous in unsanitary, moist enviro.'s
-Large cause of nosocomial infections
-colony morphology-flat, greenish/grayish irregular shape with a characteristic sweet fruity or corn tortilla smell
-Pus from this will have blue-green/yellow-green color in it
-Opportunistic
Causes:
HORSES-Abortions, repro problems
CATTLE-mastitis, abortions
DOGS & CATS-Dermatitis, cystitis, otitis
ALL-wound infections, Sx incision infection, burn infections
What are some basic facts about Campylobacter?
-GN slightly curved rods-look like flying gulls
- May stay stained together and look like spirochetes
-Very motile
-Microaerophilic to aerophilic
What are some basic facts about Campylobacter fetus?
-Affects cattle and sheep
-Called Vibrosis
-Pathology-abortions, infertility
-Acquired via breeding & AI
-Diagnosis:
Screen males via preputial wash sent off to lab. Positives are culled
What are some basic facts about Campylobacter jejuni/coli?
-Called Wet Tail in hamsters
-Causes Winter Dysentery in cattle and sheep
-Can cause enteritis, diarrhea in amny animals and humans
-Acquired via ingestion
What are some basic facts about Leptospira?
-Causes Leptospirosis in animals and people
-ZOONOTIC
-Many strain and serovars
-spirochete bacteria
What are some basic facts about Leptospira interrogans?
-Most common cause of lepto in animals
-increased incidence during years of flooding and animals exposed to wildlife
-4 of the 8 most common servars can be immunized for
-L. canicola
-L. icterohaemorrhagiae
-L. pomona
-L. grippotyphosa
-survives in reservoir hosts(rodents, raccoons, skunks)
-shed in urine
-Causes renal and hepatic damage
Causes Moon blindness in horses and abortions and infertility in cattle
What are some basic facts about Borrelia burgdorferi?
Causes Lyme disease
-Endemic on East coast, mid west and Western seaboard
-Reservoir host is the white footed mouse
-vector is the Ixodes deer tick
-Progressive disease that causes:
Intermittent shifting lameness, ADR, mild fever, depression, lameness, reduced appetite, stiffenss, reluctant to move. Progresses to arthrisitis, neuro signs such as head tilt, confusion, seizures, cardiac issues
What are some basic facts about Mycobacterium?
-Rod shaped bacteria
-causes Tuberculosis
-Don't gram stain well
-survive phagocytosis
-resistant to disinfectants, heat, pH changes, humoral defnese mechanisms (B-lmph mediated...)
-Cell mediated hypersensitivity reaction causes body to attempt to wall off bacteria and form Granulomas/ tuberculosis lesions in lungs
-Antibiotic resistant
2 types:
-Inhalation=Pulomonary TB
-Ingestion=alimentary TB
-Can cross species lines
-inversely zoonotic
What are some basic facts about Mycobacterium paratuberculosis?
-Called Johne's Disease (Yo-knees)
-Affects Cattle, sheep, goats
-Contagious, chronic and usually fatal intestinal infection of ruminents
-Causes chronic enteritis, severe diarrhea, weight loss, wasting away
-Most animals infected in 1st year of life
-Can survive in enviro for close to a year, long incubation period
What are some basic facts about Chlamydophila?
-Obligate intracellular organism that requires a living host cell to survive (like a virus)
-Infects cats, birds, humans and a few other species
What are some basic facts about Ricketsial organisms?
-Obligate intracellular organisms
-Vector important for Transmission
-Cause RMSF/Tick fever in dogs
-causes severe vascular damage due to damaging endothilial cells lining blood vessels, fever, hemorrhage due to thrombocytopenia
What are some basic facts about Erlichia canis?
Canine ehlichiosis/Tropical canine pancytopenia
-tick is vector
-Lives in canine WBC
-Fever, depression, anorexia, weight loss, ocular and nasal discharge, Bone marrow suppression
What are some basic facts about Erlichia risticii?
-New name Neorickettsia risticii
Causes POTOMAC horse fever
-Flies are most common vector
-Causes mild colic, fever, depression, anorexia, diarrhea
What are some basic facts about Anaplasma marginale?
Causes Anaplasmosis in ruminents
-Vector is ticks and other blood sucking insects
-lives on RBC's and causes them to lyse
What are some basic facts about Bartonella henselae?
-Causes CAT SCRATCH DISEASE in people-a self limiting mild disease
-Many cats, esp kittens, carry but show no signs
-Vector is fleas from cat to cat
What are some basic facts about Mycoplasmas?
-Smallest free living cells known to exist
-lack cell wall
-Stain gram negative
fragile
-resistant to cell wall inhibiting antibiotics like penicillin
-Commonly causes pneumonia as well as mastitis, arthritis
-Diagnosed with Candle jar
What are 4 common bacteria known to cause mastitis?
Staph. aureus
Staph agalactia
E. coli
Mycoplasma bovis
How is Malassezia diagnosed?
A yeast that is a leading cause of otitis
-Swab ear, roll on slide, heat fix, stain with Wrights stain
-Look for peanut men
What are some basic facts about yeasts?
-Unicellular
-Spherical to oval
-larger than bacteria
-form colonies like bacteria
-reproduce by budding
-Gram stain +
-Facultative anerobes
Diagnosis: Ear cytology swab, scotch tape prep on skin, India ink prep for Cryptococcus
What are some basic facts about Candida albicans?
-Causes Sour crop in birds, an infection of mouth, esophagus and crop
Causes oral thrush in people, vaginitis
What are some basic facts about Crytococcus neoformans?
-Produces a large gelatinous capsule making diagnosis easier
-Pigeons are carriers
-opportunistic infection acquired via inhalation.
-Cats- URI
-Dogs cryptococcal meningitis-CNS signs, seizures, ataxia, blindness
-Diagnosed w/India ink prep-capsule will look like a halo around yeast
What are some basic facts about Dimorphic fungi?
-Are molds in soil/room temp and become yeasts once they enter tissue/body temp
-cause respiratory infections when mold spores are inhaled-may become systemic depending on immune status of host
4 Types:
Blastomyces-spread systemically from resp. system to lymph nodes, skin, eyes, testicles
Histoplasma-spreads systemically within macrophages and monocytes to lymph nodes, spleen, liver and GI tract in dogs(diarrhea common)
Cocciodioides-spreads to bones, joint and CNS (seizures common)
Spriothix-Rose pickers disease, infection establishes in skin causing SQ lesions
-Diagnosed with Rads, cytology, biopsies, serology
What is the Kirby-Bauer Susceptibility test?
Test used to test certain bacteria's resistance to certain types of antibiotics
-area around disk is measured in mm.
-Resistant: no clearing
-Intermediate-May or may not work
- Susceptible-clearing around disk-drug should work