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146 Cards in this Set

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question
answer
Which organisms grow only in reduced amounts of free oxygen?
Microaerophilic Organisms
A low RBC count is a sign of _____?
Anemia
The Specific gravity of urine will be higher during what part of the day?
Morning
The Specific gravity of a liquid or solid substance is the weight of the substance as compared to an equal volume of…?
Distilled water
Which Neutrophil is known as a “Stab”?
Band
What urine preservative will false positives for Albumin seldom be encountered?
Toluene
Patient suffering from leukopenia would have what WBC count if the following cells were counted on four fields?
50-100
How are samples obtained from a urine sample that’s been preserved by toluene?
Must be pipetted from beneath the surface
What is the most common cause of poor results with Wright’s Stain?
Incorrect intervals
All of the following are normal values for Leukocytes
Bands 0%-10% Segmented 51%-67% Lymphocytes 21%-35%
The Mononucleosis Slide Test is read after 2 minutes. Agglutination in box 1 indicates what?
Positive for Infectious Mononucleosis
Which gram’s staining materials do you let sit for a minute before beginning the next step?
Crystal Violet, Gram’s Iodine and Safranine
An individual with Gonorrhea or Meningitits would have which of the following bacteria present in their system?
Gram Negative Cocci
The normal hematocrit for females is approximately what percent?
40-48%
Severe emotional upset, severe pain, and during labor are conditions that would cause?
Leukocytosis
Which organisms reproduce only in the absence of free oxygen and are killed if exposed to free oxygen?
Obligated Anaerobes
What white blood cell increases in parasitic infections?
Eosinophils
What is the least valid specimen of urine?
Random
All of the following are structures in a bacteria..
Capsule, Spore, Flagellum
What stain is used in the lab for performing a differential?
Wright’s
In the Sahli-Hellige of hemoglobin estimation, how long after the blood has been transferred to the sahli tube may the results be read on the graduate tube?
5 minutes
What lens is used to scan urine sediment
Low Power
What dilution fluid is used for performing a WBC count?
?
What leukocyte is increased when there is a shift to the left?
Neutrophils
What objective should be used for detailed study of stained bacteria smears?
Oil Immersion
What does a stained smear reveal?
Staining characteristics of bacteria present and Morphology
Dust should be removed from the microscope with
A camel hair brush
What is the most accurate urine for a microscopic exam?
Centrifuged specimen
Hematocrit is the volume of erythrocytes expressed as a percentage of the volume of plasma. T/F
FALSE
What type of bacteria are corkscrew shaped and appear singly only?
Spirilla
IF the blood and diluting fluid is drawn beyond the 101 mark for a RBC (11 for a WBC), how should the excess fluid be removed?
Touch the tip to the skin
What White Blood Cell increases in parasitic infections and allergic conditions?
Eosinophils
Next to incorrect time intervals, the most common cause of poor results with Wright’s Stain is incorrect ph of the staining fluid. T/F
TRUE
Pathogens that reproduce best at 15-20 degrees C are termed:
Psychrophilic
What objective should be used fo counting RBC’s?
High Power
An individual who has neither anti “A” or anti “B” antibodies (agglutination) would be placed in what blood group?
AB
The Mononucleosis Slide Test is read after 2 minutes. No agglutination in either box indicates what?
Negative for Mononucleosis
The normal hematocrit for males is approximately ____?
42-50%
When performing a CBC on an infant, where should you make the puncture with the hemolet?
Either Heel or Great Toe
If you have only one urine sample to centrifuge, you should:
Place a tube directly opposite to it containing an equivalent weight of water and centrifuge sample for 5 minutes at 1500 r.p.m.
When performing a RBC count, how much time should be allowed for cells to settle after loading the chamber?
3 minutes
The Mononucleosis Slide Test is read after 2 minutes. Agglutination in box 2 indicates:
Positive for Non Infectious Mononucleosis
What leukocyte is increased when there is a shift to the left?
Neutrophils
What are the 4 growth requirements for bacteria?
Temperature Oxygen Moisture Nutrition
Which bacteria when it gets into the abdominal cavity can cause peritonitis?
E. coli
What is the normal daily urine volume for adults?
800-2000ml, 1500 average
What is the most accurate means of determining blood volume?
Microhematocrit Method
What is the most accurate method of determining hemoglobin estimation?
Photometer
What in the urine would indicate a kindney disorder?
Casts
An increase in Lymphocytes in a patient with TB indicates what?
Healing
What is the normal RBC for a new born child?
5.1 +or- .9 million
What is dyscrasia of blood forming tissue caused by?
Malfunctions of the marrow and lymph tissues, commonly known as leukemia
What type of bacteria are rod shaped, appear singly in chains or palisades?
Bacilli
The following is true regarding 24 hour specimen:
Preservatives are added to the first specimen voided, patient voids and discards first specimen of urine, Urine is collected over a 24 hour period
When performing a Complete Blood Cell count, what indicates that the counting chamber is properly loaded?
There is a thin even film of fluid under the cover glass
A white blood cell whose cytoplasm is a clear to sky blue color is a….
Lymphocyte
What is the incubation period for mononucleosis?
4-21 days
If casts are present in a microscopic urine sample, it is indicative of?
Kidney Disease
When performing a finger puncture, wipe away the first drop of blood to avoid..
Dilution with tissue fluids
What urine preservative will false positives for Albumin seldom be encountered?
Toluene
A non reactive RPR for Syphilis shows no agglutination at the end of 8 minutes rotation. T/F?
TRUE
Proper sequence of using stains:
Crystal Violet, Gram’s Iodine, 95% Ethyl alcohol/acetone, Safranine
What is the largest of all normal WBC’s?
Monocyte
An individual with bubonic or Pneumonic Plague, Undulant Fever, Whooping Cough, Salmonella or Shigella would have which of the following bacteria present in their system?
Gram Negative Bacilli
Olive green to brown-black urine is caused by?
Phenols
Microscope objective lens/color/ size. Green. Yellow. Red
10. 45. 100.
A person with gastroenteritis has what bacteria present in their blood?
Gram Negative Bacilli
A person with Endocarditis, Impetigo, Septicemia, or Ostemyelitis will have what bacteria present in their blood?
Gram positive Cocci
Which Neutrophil has a nucleus that is divided into 6 or more segments?
hypersegmented
A whiteblood cell whose cytoplasm is a muddy gray-blue is ?
monocyte
Bacteria are classified according to their disease producing ability, growth requirements, morphological characteristics, colonial morphology, biochemical activity, toxins and gram’s stain reaction. T/F?
TRUE
What is the normal specific gravity of urine during a 24 hour period?
1.015-1.030
What lens is used for performing a differential?
Oil Immersion
What bacteria is spherical, appearing singly, in pairs, chains, clusters or packets?
Cocci
What is the normal value for leukocytes?
4000-11000
casts are present in large amounts, the urine is almost always:
Positive for Albumin
What leukocyte has a horseshoe or “s” shaped nucleus?
Band (Stab)
A White Blood Cell whose cytoplasm contains numerous course reddish-brown Granules which are lighter than the nucleus is a:
Eosinophils
What leukocyte is also known as a juvenile cell?
Metamyelocyte
Structures of bacteria:
Flagellum Spore Capsule
What diluting fluid is used for a Red Blood Cell count?
0.85% Sodium Chloride (Gower’s solution)
If test strips are not available to test a urine sample for Albumin, what other method may be used?
3% sulfosalicylic acid
When performing a Gram’s Stain, what is referred to as the decolorizer?
95% Ethyl Alcohol/Acetone
Which organisms grow only in reduced amounts of free oxygen?
Microaerophilic Organisms
When alpha strep is found in the blood stream it indicates?
Pneumonia
Which organisms reproduce only in the absence of free oxygen and are killed if exposed to free oxygen?
Obligate Anaerobes
What lens is used for detailed studies of bacteria?
Oil Immersion
What is the ratio of solurions mixed when diluting hydrochloric acid for the Sahli Hellige Test?
20% HCL/ 80% Distilled water
What toxin produces partial hemolysis and changes the medium to a green color?
Alpha Hemolysin
The addition of one drop of %5 Acetic acid to the urine sediment will have what effect on a urine sample?
Disintegrate any Red Cells and Make White Cells’ nucleus more visible
When performing a CBC, you are determining the amount of blood in One Cubic Millimeter. T/F?
TRUE
What is the youngest form of Neutrophil?
Metamyelocyte
If 3 or more Leukocytes are present in a microscopic urine sample, it is indicative of:
Disease in the urinary tract
How many cells are counted when performing a differential?
100
If a smear used in a differential count is to be saved for reexamination, remove the immersion oil by placing a piece of lens tissue over the slide and moisten the tissue with Xylol.
TRUE
1)      A white blood cell count with the Neutrophils number and maturity progressing towards normal indicates Recovery.
TRUE
A person with Tetanus, Diphtheria, Gas Gangrene and Food Poisoning would have what bacteria present in their system?
Gram Positive Bacilli
Which WBC does NOT decrease in acute infections?
Neutrophils
What lens is used for counting white blood cells?
Low Power
What WBC indicates healing in TB?
Lymphocytes
What stain is used in the lab for performing a differential?
Wright’s
What urine preservative may dissolve to produce false positives for Albumin?
Thymol
What indicates Albuminuria (Albumin in the urine) when testing a urine sample?
White turbidity
Which organisms reproduce only in the presence of free oxygen?
Obligated Aerobes
For a complete list of all Lab forms; Manual of the Medical Department, Chap?
23
What diluting fluid is used for performing a WBC count?
Glacial Acetic Acid or Dilute Hydrochloric Acid
When staining with Wright’s Stain, the stain and buffer should be mixed until the film appears what color?
Coppery, Metallic
Which organisms reproduce in the presence of oxygen
Aerobes
What’s the rule for counting cells when doing a blood count?
Count the cells on the lines at the top and left but not the ones at the bottom and right
A WBC whose cyto plasm has scattered large, dark blue granules that are darker than the nucleus is a Basophil
TRUE
What is the most accurate urine for a microscopic exam?
Centrifuged specimen
How long is a specimen shaken for a RBC?
3 minutes
All of the following are normally seen in a microscopic urine sample:
Spermatoza Mucous Threads, Epithelial Cells
Which toxin completely lyses the RBC, leaving a clear zone of hemolysis?
Beta Hemolysin
1)      The specific gravity of a liquid or solid substance is the weight of the substance as compared to an equal volume of Dwater.
TRUE
An individual with Pneumonia, strep throat, or Staph Aureus would have which of the following bacteria present in their system
Gram Positive Cocci
After the RPR antigen is prepared, how long may it be kept without refrigeration?
1)      One Month
What white blood cell increases in TB indicating activity in the affected area
Monocyte
Which organisms grow both in the presence of free oxygen and in an oxygen-free atmosphere?
Facultative Organisms
What type of bacteria are corkscrew shaped and appear singly only?
Spirilla
Bile in urine causes the urine to foam when shaken.
Sooo true
What type of bacteria are rod shaped, appear singly in chains or palisades?
Bacilla
Wright’s Stain must be stored in a stopper bottle in a dark place for a minimum of 30 days
TRUE
How are samples obtained from a urine sample that’s been preserved by toluene?
Must be pipetted from beneath the surface
Pathogens that reproduce best at 15-20 degrees C are termed what?
Psychrophilic
After the 4 fields are counted during a white blood cell count, you should multiply by 50
TRUE
1)      Pathogens that reproduce best at 50-55 degrees C are termed Thermophilic
TRUE
After the five fields are counted when performing a RBC count, you should multiply by 10,000
TRUE
What WBC increases in parasitic infections and allergic conditions?
Eosinophils
The percentage of eosinophils, monocytes and lymphocytes generally do not increase in acute infections
TRUE
What causes urine to have a milky appearance?
Chyle
A Gram positive organism will leave a ___ color reaction to a gram’s stain?
Dark blue-black
Which Neutrophil has a nucleus that is divided into 2, 3, 4, or 5 segments?
Segmented
A smear that reveals large numbers of pus cells with varying numbers of intracellular and extracellular gram neg, bean shape cocci in pairs indicate Gonorrhea
TRUE
When performing a Gram’s Stain, what is referred to as the primary stain?
Crystal Violet
A person with boils or furuncles will have what bacteria present?
Gram Positive Cocci
What organisms grow only in reduced amounts of free oxygen?
Microaerophilic Organisms
The number of cells counted in each field should not vary by more than how many cells from the field containing the fewest to the field containing the most?
20
What oil solvent should be used to remove dried oil from lenses?
Xylol
When performing a Gram’s Stain, what is referred to as the counter stain?
Safranine
After the RPR antigen is prepared, how long is it good for if kept refrigerated?
3 months
Which toxin is a soluble protein poison that is secreted by the living cell?
Exotoxin
Once blood and diluting fluid is drawn into the pipet, in what direction and how long should the pipet be shook?
5 min NOT in the direction of the long axis
Agglutination is present in the absence of antibodies.
TRUE
Which toxin comprises part of the cell wall and is released by autolysis of the bacterial wall?
Endotoxin