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63 Cards in this Set
- Front
- Back
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What are the 4 anticoagulants used in preparing RBCs?
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1. ACD (acid citrate dext)
2. CPD (citrate phosph dext) 3. CP2D (cit-phosph-doubdext) 4. CPDA-1(cit-ph-dext-adenine) |
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What are the storage times of each anticoagulant?
What temp is indicated? |
ACD: 21 days
CPD: 21 days CP2D: 21 days CPDA-1: 35 days All: 1-6 degrees C |
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What 3 additive solutions are used for RBCs?
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1. Adsol
2. Nutricel 3. Optisol |
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What are the storage times for additive solutions?
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42 days
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Why is CPD better than ACD?
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2,3-DPG levels lower in 1st week with ACD; they lower in the 2nd week with CPD.
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How does CPDA-1 enhance storage?
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Adenine drives glycolysis to make more ATP and lengthens RBC survival.
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What is the purpose of additive solutions?
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-Preserve packed RBCs that have no plasma.
-Decrease viscosity of unit |
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What 5 changes occur in blood with storage?
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-pH decrease
-Glucose consumption decrease -Lactic acid buildup -ATP decrease -Reversible 2,3-DPG decrease |
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What is the shelf life of RBCs or WBCs in:
-ACD -CPD -CPDA-1 -AS |
ACD: 21 d CPD: 21 d
CPDA-1: 35 d AS: 42 d |
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What is the shelf life of irradiated RBCs or WB?
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Original outdate or 28 days, whichever comes first.
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What is the shelf life of an OPEN system of RBCs or WB?
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24 hrs, if at 1-6'C
4 hrs if at RT |
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What is the shelf life of washed RBCs?
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24 hrs
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What is the shelf life of frozen RBCs?
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10 yrs
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What is the shelf life of any platelet unit?
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5 days
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What is the shelf life of pooled platelets?
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4 hrs - they have to be opened
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What is the shelf life of FFP at
-minus 18'C -minus 65'C |
-18: 1 yr
-65: 7 yr |
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What is the shelf life of PF24?
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same as FFP
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What is the shelf life of Cryo?
-Frozen -Thawed -Pooled |
Frozen: 1 yr
Thawed: 6 hrs Pooled: 4 hrs |
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What temp must cryo be thawed at?
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20-24'C
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What is the shelf life of WBCs?
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24 hrs
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What is the shelf life of PPF (plasma protein fraction)?
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5 yrs
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What is the indication for WB? What is the standard vol?
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Volume expansion; 450-500 ml
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What is the indication for irradiation?
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To prevent GVHD
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What is the indication for RBCs? What is the standard vol?
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To increase O2; anemia.
250-300 mL |
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What is the indication for leukoreduced RBCs?
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To prevent febrile reactions
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What is the indication for Washed RBCs?
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IgA Negative persons, PNH
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What is the purpose of frozen RBCs?
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Rare phenotypes
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What is the indication for Platelets?
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Thrombocytopenia, DIC, bleeding.
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What is the indication for single donor platelets?
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Platelet refractoriness (reaction to HLA on platelets)
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What are the volumes of:
-Random donor platelets -Single donor platelets |
RD: 50-70 ml
SD: 200-400 ml |
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What transfusion criteria applies to SD platelets that doesn't to RD?
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Must be HLA compatible
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What is the storage temp of all platelets?
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20-24'C
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What is the indication for FFP?
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Coagulopathy, liver disease, DIC, massive transfusion.
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What is the typical vol of FFP?
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200-250 ml
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What is the indication for Cryoprecipitate?
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-Hemophilia A
-vWD -Fx Eight deficiency -Fibrin sealant, hypofibrinogen |
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What is the typical volume of cryoprecipitate?
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10-25 mL
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What is teh typical indication for WBCs?
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Neutropenia < 500 cells/uL
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What is the indication for PPF?
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Plasma volume expansion
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Which components must be ABO compatible?
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WB, RBCs, FFP, WBCs.
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Which components do not have to be ABO compatible?
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Platlets, Factor concentrates, and Albumin.
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What are the physiological requirements of a blood donor?
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At least 110 lbs, pulse between 50-100 bpm, Hct at least 38%
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What test result for Hep B defers a donor?
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Pos HBsAg since 11th bday
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When does malaria history disqualify a donor?
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If treated in past 3 years
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What 2 circumstances defer a donor for one year?
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-Blood transfusion
-Positive syphilis serologic test |
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When does aspirin disqualify a donor?
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If attempting to give platelet pheresis, can't take within 3 days prior to donation.
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How much time must elapse between consecutive blood donations?
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8 weeks.
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What permanently defers a blood donor?
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-Hemophilia A/B history
-von Willebrand's Disease -Severe thrombocytopenia -Pos HIV test |
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How long is a donor deferred if received live viral vaccines?
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2 weeks
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Which vaccines carry a 4 week deferral?
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-German measles (rubella)
-Chicken pox |
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What are the physiologic requirements for autologous donation?
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Hgb of 11g/dL
Hct of 33% or higher |
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Quick; Storage temps are:
-Whole blood/RBCs -Platelets |
WB = 1-6'C
Plt = 20-24'C |
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What 7 tests must be done on donor units?
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1. Syphilis
2. Anti-HIV 1/2 3. HIV antigen 4. Anti-HTLV 1/2 5. HBsAg 6. anti-HBc 7. anti-HCV |
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What is the required Hct of PRC?
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Not >80%
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What is the new expiration date of irradiated products?
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28 days, unless the anticoag used was only 21 days anyway.
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What is the max level of WBCs in leukoreduced RBCs?
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5 x 10^6
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What is the platelt count in:
-Random donor units? -Single donor units? |
RD: 5.5 x 10^10
SD: 3.0 x 10^11 |
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How soon does FFP have to be prepared after collection?
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Within 8 hrs
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What is Cryo prepared from?
What does it contain? |
FFP
Fibrinogen and Platelet Factors |
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What is the purpose of buffy poor units?
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To leukoreduce a unit and prevent anyphylctic reaction in IgA deficient or PNH patients.
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What are the time limits for preparing:
-Platelets -FFP -Cryo |
Platelets: within 6 hrs
FFP: within 8 hrs Cryo: within 6 hrs of thawing, 4 hours if ffp is pooled. |
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What populations would be eligible for CMV-neg units?
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-Pregnant women
-Neonates, premature infants -Immunocompromised pts -chemo patients |
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Which blood components are at risk for CMV transmission?
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RBCs and platelets
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When CMV neg units are not available, what is an appropriate alternative?
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leukoreduced.
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