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10 Cards in this Set
- Front
- Back
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Describe glucose fermentation test |
Phenol red is a pH indicator and turns yellow as acid is present. Acid and gas can be products |
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What are the 2 gasses released during sugar fermentation |
Carbon dioxide ,hydrogen sulfide |
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What are the three sugars used in a sugar fermentation test |
Lactose. Glucose. Sucrose |
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What is the purpose of the TSI test and what does it stand for |
Triple sugar iron agar Used to identify enteric bacteria that metabolize sucrose, lactose, and glucose and release hydrogen sulfide |
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What is the purpose of the IMVIC series |
to differentiate the enteric organisms |
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What are the four parts of the IMVIC series |
1. Indole - identify bacteria that can metabolize tryptophan 2. methyl red - identify acid fermentation 3. Voges Proskauer - identify butanediol product of glucose fermentation 4. Citrate - identify bacteria that use citrate as a sole source of carbon |
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Described the indole test |
Stab a SIM agar that has tryptophan with a bacteria of interest Add Kovacs reagent The development of a cherry red colour at the top means the bacteria can metabolize tryptophan |
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What test would you use SIM agar for |
H2S / indole / motility detection |
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Describe the results you would see with the citrate test |
green color being negative results Bromothymol blue is positive |
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What indicates a positive MR test |
A persistent red ring |
