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10 Cards in this Set

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  • Back
What is coagulation instrumentation based on?
detection of an endpoint in the coag cascade when a clot forms.
When are the 2 most common times you would use coag?
1. To monitor anticoag therapy
2. before a surgical procedure
what are 2 common coag tests?
PT: prothrombin time
APTT: actvtd ptl thromboplastin time
also Factor assays
what is PT?
the time it takes for a clot to form.
What are 3 ways to measure coagulation?
1. Manual
2. Mechanical
3. Optical
What is the manual method that we'll use next year called?
What's the principle of the fibrinometer?
1. Automatic pipetter shoots sample into cup, time starts.
2. Heater starts and 1 probe agitates. 1 probe stationary.
3. When clot forms, agitation stops; current flows; time stops. Endpoint detected!
What's an advantage/disadvantage of the mechanical method?
-More precise btwn techs
-More cumbersome/frustrating method.
What are some different photometric coag methods?
1. Light scatter optical
2. Turbidometric
3. Viscosity
4. Absorbance /or fluorescence.
What's a chromogenic assay based on?
the fact that a chromophore when bound to pna doesn't shine, but when a coag factor cleaves it, it does shine. allows for factor assays.