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14 Cards in this Set
- Front
- Back
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What is the catalase reaction for Staphylococcus?
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Positive
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Which tests which differentiate between S. aureus and the Coagulase negative Staphylococci
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Coagulase
Staphylatex DNAse Thermostable nuclease test |
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Identify this bacterium:
gpc catalase positive Staphylatex test positive |
Staphylococcus aureus
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What test will differentiate between S. saprophyticus and other Staphylococci?
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Novobiocin resistance
S. saprophyticus is resistant to novobiocin; other Staphylococci are susceptible. |
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What is the DNAse reaction of Staphylococcus epidermidis?
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negative
Of the Staphylococci, only S. aureus is positive |
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What type of coagulase is detected by the slide coagulase test?
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Bound coagulase.
The tube test detects both free and bound coagulase. |
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Describe the principle of the Staphylatex test.
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Latex beads are coated with fibrinogen and anti-Protein A.
S. aureus has coagulase which converts the fibrinogen to fibrin, forming a clot. It also has the Protein A antigen -- when the antibody reacts with the antibody you get agglutination. Overall, a positive reaction is clumping. |
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How does Micrococcus compare to Staphylococcus?
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Both are gpc, catalase positive.
Micrococcus has a brighter yellow pigment. |
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How does Stomatococcus compare to Staphylococcus?
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Both are gpc.
Staphylococcus is catalase positive; Stomatococcus is catalase variable. Stomatococcus colonies are sticky as compared to Staphylococcus. |
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Where are these bacteria normal flora?
a. Staphylococcus aureus b. Coagulase negative Staphylococcus c. Stomatococcus |
a and b. skin and mucus membranes (mouth and vagina)
c. mouth |
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What type of infection is associated with Staphylococcus epidermidis?
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SBE when a prosthetic heart valve is involved.
Bloodstream infections |
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Name diseases associated with S. aureus.
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Skin diseases (e.g. acne, folliculitis, impetigo, boils, scalded skin syndrome)
toxic shock syndrome staph food poisoning wound infections osteomyelitis pneumonia |
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What infection does S. saprophyticus cause?
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UTI in sexually active 18-25 year old females
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What is the first choice for antibiotic treatment of S. aureus?
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Methicillin
If methicillin resistant, use vancomycin. For VISA, GISA, VRSA strains, use Synercid or Zyvox. |
