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20 Cards in this Set
- Front
- Back
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Blood Agar
(Sheep Blood Agar) |
Non-selective, Differential medium
Supports growth of most medically significant bacteria; useful for primary plating, subculture, and detection of hemolysis Ingredients: Trypticase soy agar and defibrinated sheep blood |
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Chocolate Blood Agar (CBA)
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Enriched Medium
Recommended as primary plating for CSF, eye cultures, GC cultures, and any other specimen that may contain fastidious organisms Ingredients (nutritive): Variation of SBA, lysed RBCs (for hemoglobin) and NAD (nicotinamide adenine dinucleotide) |
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Colistin Nalidixic Acid Agar (Columbia CNA)
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Enriched-selective Medium
For isolation of Gram-positive cocci from specimens with mixed flora Ingredients: sheep blood, colistin and naladixic acid to inhibit Gram-negative bacilli |
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Eosin-Methylene Blue Agar (EMB)
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Selective-Differential Medium
To isolate and identify Gram-negative bacilli Ingredients: lactose and sucrose for fermentation patterns Eosin Y and methylene blue for inhibition of Gram-positive bacteria |
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Enriched Media
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Contains nutrients needed for growth of fastidious organisms
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Enrichment Media
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Selects for growth of a particular organism(s) while inhibiting growth of other bacterial flora present
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Nutritive Media
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Supports the growth of most nonfastidious bacteria
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Selective Media
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Inhibits the growth of some organisms while allowing for the growth of others
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Differential Media
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Allows differentiation of groups of organisms; contains factors that can be used to distinguish certain characteristics that aid in the ID of organisms
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Upper Respiratory Tract
Routine Media |
SBA for recovery of routine aerobes
EMB for GNR CBA for fastidious organisms Thioglycollate broth for recovery of all organisms, including anaerobes CNA GC Agar (Thayer-Martin) for Neisseria |
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Lower Respiratory Tract
Routine Media |
SBA
EMB CBA |
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Superficial wound/abscess, drainage of fluid and soft tissue cultures
Routine Media |
CBA
BA EMB CNA Thio |
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Routine Stool Culture Media
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SBA
EMB HE CIN CAMPY Agar SMAC Selenite F Broth TCBS CNA |
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Routine Media for Urine
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BA
EMB Thio for suprapubic aspirate |
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Routine Media for CSF
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CBA
BA Thio |
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Fluid Testing (sterile body fluids)
Routine media |
CBA
BA EMB CNA Thio Bactec Blood Culturing System |
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Thioglycollate Broth
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supports growth of most bacteria, including anaerobes
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MacConkey (MAC)
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Selective-Differential Medium
to isolate and ID G- bacteria, especially enteric bacteria Differentiates lactose fermenters from non-fermenters Ingredients (non-nutritive) Lactose for fermentation pattern Bile salts and crystal violet inhibitmost G+ organisms |
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Modified Thayer-Martin agar
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Enriched-Selective Medium
To recover Neisseria gonorrohoeae and N. meningitidis from specimens with mixed flora Ingredients Growth Factors: hgb, vitamins, cocarboxylase, diphosphopyridine nucleotide and glutamine Cornstarch to absorb inhibitory substances Antibiotics: Vancomycin to inhibit G+C Colistin to inhibit GNB Nystatin prevents growth of fungi TMP prevents Proteus from swarming |
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Hektoen-enteric agar (HE)
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Selective Differential Medium
To isolate enteric pathogens from feces Ingredients: (non nutritive) Bile salts-inhibit G+ and NF Lactose, sucrose, and salicin to detect fermentation Ferric salts (sodium thiosulfate and ferric ammonium citrate) detect H2S Bromthymol blue and acid fuchsin (pH indicators; yellow color with acid) |
