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140 Cards in this Set
- Front
- Back
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Gram negative diplococci kidney bean appearance
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Neisseria
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Neisseria catalase test
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positive
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what 2 agars for Neisseria
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chocolate and MTM
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superoxol for Neisseria
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positive
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sugar ferm for Neisseria
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dextrose only
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two susceptibility tests for Neisseria
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disc diffusion or Etest
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two susceptibility tests for Neisseria
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disc diffusion or Etest
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causative agent of meningococcal meningitis
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Neisseria meningitidis
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causative agent of meningococcal meningitis
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Neisseria meningitidis
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reservoir for N. meningitidis
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humans
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reservoir for N. meningitidis
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humans
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what color under colony for N. meningitidis
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green
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what color under colony for N. meningitidis
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green
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how many serogroups for N. meningitidis
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12
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how many serogroups for N. meningitidis
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12
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two susceptibility tests for Neisseria
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disc diffusion or Etest
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causative agent of meningococcal meningitis
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Neisseria meningitidis
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reservoir for N. meningitidis
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humans
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what color under colony for N. meningitidis
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green
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how many serogroups for N. meningitidis
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12
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serogroups for N. meningitidis based on what
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capsular polysaccharides
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what 2 serogroups of N. meningitidis responsible for most disease in US
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B and C
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sugar ferm. for N. meningitidis
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dextrose and maltose
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recovery of pathogenic Neisseria from what body sites for chocolate vs. MTM
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chocolate (sterile) and MTM (nonsterile)
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vancomycin inhibits what organisms
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Gram positive
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colistin inhibits what organisms
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Gram negative
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nystatin inhibits what organisms
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fungi
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trimethoprim inhibits what organisms
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Proteus sp.
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positive result for direct plate method (oxidase test)
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blue color change
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what does CTA stand for
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cystine tryptic agar
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CTA sugars are used to detect ________ utilization in fastidious organisms.
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carbohydrate
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members of Neisseria produce acid from carbohydrates by what (oxidation or fermentation)
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oxidation
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positive test for CTA sugar fermentation
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change from yellow to orange/red
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what does a red color change after zinc addition indicate
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organism did not reduce nitrate
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is reagent A sulfanilic acid or N-dimethly-alpha-naphthylamine
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sulfanilic acid
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Gram negative coccobacilli to small rods (4 genus)
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pasteurella, actinobacillus, haemophilus, bordetella
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pasteurella, actinobacillus, haemophilus, bordetella oxidase and catalase tests
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both positive
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bordetella (oxygen requirement?)
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strict aerobe
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pasteurella, actinobacillus, haemophilus, bordetella (which are commensals that live in upper respiratory tract)
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all
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pasteurella, actinobacillus, haemophilus, bordetella which has an extracellular slime layer
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actinobacillus
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pasteurella, actinobacillus, haemophilus, bordetella- which exhibits bipolar staining (safety pin appearance)
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pasteurella
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pasteurella, actinobacillus, haemophilus, bordetella - which has a typical musty odor as a diagnostic feature
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pasteurella multocida
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pasteurella, actinobacillus, haemophilus, bordetella- which is usually an oral pathogen
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actinobacillus actinomycetomcomitans
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stain for P. multocida, A. ureaea, and A. actinomycetomcomitans
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Giemsa stain
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most bacteria stained with Giemsa stain what color
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blue
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procedure for Giemsa stain
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prepare smear, air dry, absolute methanol for 5 minutes, Giemsa stain for an hour, wash with phophate buffer pH 6.4
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significant cause of meningitis particularly in children
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haemophilus influenzae
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#1 cause of newborn meningitis
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haemophilus influenzae
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requires growth factors X and V; requires only V; does not require either
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haemophilus influenzae, haemophilus parainfluenzae, bordetella
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cause of whooping cough
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Bordetella pertussis
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media for Bordetella
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Bordet-Gengou
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kennel cough
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B. bronchiseptica
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determines an organism's requirement for x factor
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porphyrin (ALA) test
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porphyrin (ALA) test used for what organism
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Haemophilus sp
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positive result for ALA test
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red fluorescence under UV light or red color after Kovac's reagent added
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what does a positive ALA test indicate
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organism doesn't require X factor
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small, gram negative coccobacilli
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Brucella
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what plate would you grow Brucella on
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blood agar plate (5-10% CO2)
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oxidase, catalase, and urease tests for Brucella
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all positive
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Biosafety level 3
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Brucella
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2 patient populations for brucella
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working with animals, ingesting unpasteurized dairy products
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most common agen seen in ppl who ingest unpasteurized dairy products
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B. melitensis
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best sample for recovery of Brucella
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blood culture
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night sweats indicative of what gram negative bacteria
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Brucella
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leading cause of brucellosis deaths
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endocarditis
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of Francisella and Brucella, which can be transmitted from person to person
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Francisella
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small, pleomorphic gram negative coccobacilli
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Francisella
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what does Francisella require for good growth
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cysteine supplementation
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what 3 agars good for francisella growth
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chocolate, MTM, BCYE
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what does BCYE stand for
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buffered charcoal yeast extract
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causative agen of rabbit fever
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Francisella tularensis
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F. tularensis is transmitted how
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insect vector or direct contact/inhalation
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most frequently presents as ulceroglandular disease
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Francisella
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DOC (2) for Francisella
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tetracycline and quinolones
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DOC (2) for Brucella
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gentamicin and doxycycline
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catalase, oxidase, and urease test for Francisella
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catalase weakly positive, oxidase negative, urease negative
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where does legionella love to live
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water
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thin gram negative bacilli, stains poorly
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Legionella
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what counterstain should you use with legionella
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carbol fuschsin
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Legionella requires what for growth
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L-cysteine
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colonies exhibit a cut glass appearance on BCYE
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Legionella
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growth stimulated by iron
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Legionella
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what does IFA stand for
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indirect fluorescent antibody
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browning of trytosine containing agar (one example of + and -)
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positive is L. pneumophila, and negative is L. micdadei
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gram positive bacilli, high mycolic acid content
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Mycobacterium
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2 groups for Mycobacterium
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M. tuberculosis complex and non-tuberculoid mycobacteria
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Group I Mycobacterium
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photochromogens- pigmented only upon light exposure (yellow to orange)
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Group II Mycobacterium
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scotochromogens- pigemented
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Group III Mycobacterium
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non-photochromogens- never pigmented
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Group IV Mycobacterium
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rapid growers (less than 7 days)
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growth rate for Group I, II, III for Mycobacterium
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2-8 weeks
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Tween 80 hydrolysis used for what
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Mycobacterium
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M. phlei vs. smegmatis (Groups)
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phlei is scotochromogen and smegmatis is non-chromogenic
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what is a negative test for ornithine decarboxylase
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no color change (remains purple), or color change to yellow
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are klebsiella and shigella motile or non-motile
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non-motile
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what does HE agar stand for
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hektoen enteric agar
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what are the inhibitory substances in L-eosin methylene blue
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eosin Y, methylene blue
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what are the inhibitory substances in HE agar
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bile salts, acid fuschin, bromthymol blue
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what are the inhibitory substances in shigella-salmonella agar
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bile salts, brilliant green
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what are the inhibitory substances in bismuth sulfite agar
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bismuth sulfite, brilliant green
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what is CIN agar used for (genus)
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Yersinia
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what reagent do you add for indole test
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Kovac's reagent
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how do you get Vibrio
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eating raw or improperly cooked seafood
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what media is used for Vibrio
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TCBS
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what does TCBS stand for
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thiosulfate-citrate bile salts-sucrose
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what does yellow on TCBS mean? Green/blue?
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fermentation of sucrose=V. cholerae; blue/green=V. parahaemolyticus
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media for Campylobacter?
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Campylobacter Blood Agar Plate
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what bacteria is associated with cystic fibrosis
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pseudomonas aeruginosa
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grape like odor
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pseudomonas aeruginosa
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What two common clinical forms for legionellosis
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legionnaires' disease and Pontiac fever
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Weil's disease, canicola fever, rats a major host
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Leptospira
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all parasitic, cause relapsing fever
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borrelia
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Lyme disease
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Borrelia
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what does ECM stand for and what is it clinical evidence for
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erhthema chronicum mirgrans, borrelia
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Peptone broth with and without NaCl is used for what
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Vibrio
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O/129 disks used for what
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Vibrio
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MR-VP used for what
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Enterobacteriaceae
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what does PRAS stand for
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pre-reduced anaerobically sterilized media
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anaerobic spore formers
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Clostridia
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alpha toxin causes gas gangrene
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clostridium perfringens
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causes antibiotic associated diarrhea
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Clostridium difficile
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non-pathogenic form of C. botulinum
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C. sporogenes
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double zone of hemolysis on SBA and stormy fermentation in tubed milk medium
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C. perfringens
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cooked meat medium used for what
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Clostridia
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Hemin-Vitamin K sheep blood agar used for what
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Clostridia
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EYA plate used for what
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Clostridia
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what does EYA stand for
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egg yolk agar
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what were PYG tubes used for
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clostridia
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what does PYG stand for (what organism)
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peptone yeast glucose, clostridia
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what does PYL stand for (what organism)
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peptone yeast lactose; clostridia
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what was cystein-tellurite medium used for
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corynebacterium
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loeffler's slants/Loeffler's methylene blue stain used for what organism/structure
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corynebacterium-metachromatic granules
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what causes Hansen's disease
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mycobacterium
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Lowenstein Jensen culture for what
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mycobacterium
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Middlebrook agar slants used for what
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Mycobacterium
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Ziehl-Neelsen stain used for what
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Mycobacterium
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paraffin baiting used for what
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Nocardia
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sulfure granule associated with what
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actinomyces
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what stain used for rhodococcus
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kinyoun method
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gram stain for actinomyces, nocardia, rhodococcus, and stretomyces
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gram positive
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