Clinical Chemistry - Chapter 5 - Analytic Techniques

Front 1

Monochromator:

Back 1

part of spectrophotometer that isolates wavelengths

Front 2

Photodetector:

Back 2

Part of spectrophotometer that converts transmitted radiant energy to an equivalent amount of electrical energy

Front 3

Stray light:

Back 3

wavelengths outsidethe band transmitted, usually caused by scratched on optical surface

Front 4

Atomic absorbancespectrophotometers are used to measure:

Back 4

concentrations oftrace metals that are not easily excited (lead, mercury, etc)

Front 5

Flame photometry was used to determine:

Back 5

concentration of Na,K, and Li; replaced by ion-selective electrodes

Front 6

Fluorometry

Back 6

analytical techniquethat uses fluorescence to detect and identify small samples of substance;source emits short-wavelength high energy excitation light

Front 7

Chemiluminescence:

Back 7

part of chemicalenergy generated produces excited intermediates that decay to ground state withemission of photons; no excitation radiation or monochromators are required

Front 8

Turbidity measures:

Back 8

concentration ofparticulate matter

Front 9

Nephelometry differs from turbidity in that:

Back 9

it uses a different angle of measurement

Front 10

pH reference electrode:

Back 10

Calomel electrode

Front 11

Electrophoresis is the:

Back 11

migration of chargedsolutes/particles in an electrical field

Front 12

Osmometry measures:

Back 12

concentration ofsolute particles in a solution