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9 Cards in this Set
- Front
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Movement of compound between mobile and stationary phases depends on the compounds affinity for things like: |
- polarity -ionic charge -molecular weight
and so which phase it relates most to. (e.g. polar molecules will be found in the most polar phase). |
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5 main types/formats of chromatography: |
-Thin Layer Chromatography -Gas Liquid Chromatography -Liquid Chromatography/High Performance Liquid Chromatography. -Supercritical Fluid Chromatography. -Ion Chromatography |
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3 variations of liquid chromatography/HPLC: |
- Partition (normal phase/reverse phase) -Size Exclusion -Ion Chromatography |
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Principle of TLC: |
A mobile phase, usually a solvent, is used sometimes in an applied field to move compounds over a flat surface coated with a suitable stationary phase. |
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TLC Applications: |
-to determine impurities in organic products. -basic identity check on raw chemical materials. -identification of a compound following an organic reaction, in comparison to the initial substrates. |
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TLC Analysis: |
Qualitative and can be SEMI-quantitative Based on calculation of Rf value. |
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Calculation of Rf value: |
(distance moved by compound)/(distance moved by solvent front). |
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Development of TLC plates: |
Either UV light (at 254nm) or use of reagent. |
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Reagents for developing TLC plates: |
1) Iodine Vapour (organic compounds) 2) Potassium Permangenate (sugar or sugar-like compounds). 3) Ninhydrin solution (primary and teritary amines). 4) Alkaline Tetrazolium Blue (steroids). |
