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27 Cards in this Set
- Front
- Back
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What is ADsorption?
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Adsorption is the attachment of molecules onto the surface of a solid. The attachment occurs when secondary interactions form between the molecules and atoms at the surface of the solid.
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What is ABsorption?
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The incorporation of particles into the body of another material.
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What is Adsorption Chromatography?
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A widely used technique for separating and identifying the components of mixtures.
It is also commonly used to quantitatively determine the concentrations of the components. |
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What is a stationery phase?
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The material onto which the components of the substance are adsorbed
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Mobile phase?
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The liquid (or gas) that moves across the stationary phase carrying the mixture
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4 different types of adsorption chromatography?
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Thin Layer Chromatography (TLC)
Column " Gas - Solid " (GSC) Liquid - Solid " (LSC) |
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Common materials used for solid stationary phase?
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Silica - SiO2
Alumina - Al2O3 |
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Normal phase?
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The polarity of stationary phase is greater than polarity of mobile phase
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Reverse Phase?
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Polarity Stationary phase less than polarity of mobile phase.
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Reverse phase uses what stationary phase?
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An ODS particle with non-polar C18 groups on the surface.
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Desorption?
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Where a substance returns to the mobile phase
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Eluted?
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Act of washing down a substance in column chromatography
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Elutent?
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Mobile phase in column chromatograpy
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How do you increase sample amount in TLC?
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The solvent is allowed to evaporate and the spotting procedure is repeated
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What is a Chromatogram?
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A visible record showing the results of a chromatograpy process
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How are colorless organic compounds made visible in TLC?
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1. By placing the plate under UV light, making the dots appear blue
2. Plate is sprayed with a chemical reagent |
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What is a standard?
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An identified substance that has moved across the stationary phase by a known distance, using the same mobile and stationary phase.
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What is the retardation factor?
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A ratio value that allows for the identification of a substance.
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Calculate Retardation factor
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Compound Dist
-------------------- Mobile Dist |
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What gas is used for mobile phase in Gas - Solid?
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Helium or Nitrogen
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What is mobile phase in gas-solid called?
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A carrier Gas
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Gas - solid chrome? Used for?
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Used to separate mixtures of volatile organic compounds. Contains uniformly sized particles.
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Qualitative Identification? (Gas -Solid)
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Comparing rendition time values with known compounds.
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Quantitative analysis? (Gas -Solid)
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Comparing the peaks on chromatogram to fit the ratio of amounts in mixture.
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High Pressure Liquid - Solid Chomatograpy? (HPLC)
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Used for mixtures with components with high boiling points.
Mobile phase enters under high pressure pump |
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Mobile phases? From Non-polar to polar
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Cyclohexane
Ethyl Ethanoate Propanol Ethanol Water Ethanoic Acid |
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Solvent Front?
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The point where the mobile phase reaches
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