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17 Cards in this Set
- Front
- Back
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What you want for microscope
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down lambda and RP and up NA
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Nanometer
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Wavelengths measurement( lambda)
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Micrometer-
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UM= 1/1000 of mm
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Resolution-
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ability to see separation short wavelengths= better RP and up in energy level
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NA
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Light gathering ability more light= you see it more for smaller lambda and RP
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Bright feild micro
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Background as light organism is dark
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Dark feild
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Background as dark organism is light best for colorless org ex Treponema pallidum( Sypillus) Used with a condenser
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Phase contrast
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special condesor accentuates diff in refractive indexbest for viewing live org
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Florescent
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UV light and dyes to floresce it decreases lambda and RP
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E micro
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Electrons as illuminator(not light) or energy source and magnets to focus( not Glass) for decresed lambda and RP
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TEM and SEM
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transmission is cross sectional and scanning is 3-d surface
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Wet mounts
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for LIVE org, not stained so lower condenser b/c you close diaphragm to up contrast but you decrease lambda and increase RP
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Smears
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for bacteria used for heat fixation and stained for contrast dead bac takes stain better,
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CAtionic ( basic stain
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stain used more. b/c cells are neg charged so it is attrated so it stains m/o and not backround.
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Gram stain
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(VIAS) Gram positive = purple( thick peptido like bacillus antracis or anthrax Gram neg = pink and LPS mem b/c alcohol dissolves it and end up pink like e coli and N gonnorhaea
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Acid fast stain
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AF pos= red and Mycobacterium (TB) and nocardia AF neg= green or blue all other bacteria is pos it holds red( fastening) B/c of waxy cell walls
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acid fast steps
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cover smear with tissue , red stain and use warm water ti drive stain through wall, wash with alcohol counterstain with green green or blue is neg red is positive
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