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83 Cards in this Set

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What is the size range for prokaryotes?

0.2-2.0 um

Do prokaryotes have a nucleus or nuclear membrane?

No

Do prokaryotes have organelles?

no

Do prokaryotes have a cell wall?

Yes, with peptidoglycan

Name 3 descriptions of the DNA in prokaryotes.

Singular. Circularly arranged. No histones

How do prokaryotes divide?

binary fission

What is the size range of eukaryotes?

10-100 um

Do eukaryotes have a nucleus?

Yes, within nuclear membrane

Do eukaryotes have organelles?

Yes

Do eukaryotes have a cell wall?

Yes, but when present, very simple. Not found in animals or helminths.

How do eukaryotes reproduce?

mitosis

Name 3 attributes of eukaryotic DNA

Multiple. Linear. Contains histones

What is morphology?

Size, shape, and arrangement

What is arrangement based on?

Plane of division

What do you call a pair of cocci that divides in one plane?

diplococci

What is a chain of cocci that divides in one plane?

streptococci

What is a cluster of cocci that divides in all 3 planes of division?

staphylococci

What divides in two planes and is in a group of 4?

tetrad

What divides in 3 planes and is in a group of 8?

sarcinae

What divides in multiple planes and forms grapelike clusters or broad sheets?

staphylococci

What do you call a group of bacilli that are oval, almost appearing to be cocci?

coccobacilli

What do you call bacteria that look like curved rods?

vibrio

What do you call spirilla that is not as rigid and has more twists and turns?

spirochetes

Name 3 functions of bacteria cell wall.

#1 provides shape


#2 prevents osmotic lyses


#3 helpful identifying organism

What allows us to be able to differentiate between gram+ and gram-?

peptidoglycan

What are the 2 main sugar units that make up peptidoglycan?

NAG and NAM

What connects rows of sugar units that make up peptidoglycan?

polypeptides

Gram+:


1. Peptidoglycan?


2. Teiochoic acid?


3. LPS membrane?


4. Does it produce exotoxins?

1. Thick


2. Yes


3. No


4. Yes

How is teichoic acid charged?

Negatively

What does teichoic acid's charge allow Gram+ bacteria cells to do?

Bond and move cations (positive ions) into and out of the cell

Name four roles of teichoic acid.

1. role in cell growth


2. prevents cell wall damage


3. helps prevent lysis


4. identification of bacteria

Gram- cell wall


1. Peptidoglycan layer?


2. Teichoic acid?


3. LPS membrane?


4. Produces:?

1. Thin


2. No


3. Yes, outer membrane is made of LPS


4. Produces endotoxins

What is on the outer membrane of a Gram- bacteria?

Lipopolysaccharide (LPS)

What is LPS made of?

1. Lipid A


2. core polysaccharide


3. O polysaccharide



What is Lipid A's function?

endotoxin

What is O-polysaccharide's purpose?

Identification

Why does the third step, decolorization (with alcohol), leave Gram- bacteria colorless?

The alcohol dissolves the outer membrane of the cell, also creating small holes in thin peptidoglycan layer. This allows the crystal violet to diffuse out of the cell.

Why does decolorization not leave Gram+ bacteria colorless?

The alcohol dehydrates thick peptidoglycan, making it impermeable to the crystal violet.

What are the only prokaryotes known to have sterols in their plasma membrane?

Mycoplasma

Why won't an antibody targeting cell walls work on mycoplasma?

Very little to no cell walls

Why were mycoplasmas first thought to be viruses?

Because they are very small, and pass through bacterial filters

What is the scientific name for primary atypical pneumoniae?

Mycoplasma pneumonia

What makes up the wall of Mycobacterium?

Mycolic acid

Mycobacterium (tuberculosis) is the only bacteria to hold:

fuschia stain

What enzyme targets Gram+ cells by breaking apart the bond of NAG and NAM?

Lysozyme

Where is lysozyme found in human body?

body secretions (tears, mucus, etc)

What prevents polypeptide formations in Gram+ cells?

penicillin

What is the sticky, viscous sugar coat excreted by prokaryotes?

glycocalyx

What is a firm, attached presence of glycocalyx known as?

capsule glycocalyx

What is an unorganized, loose layer of glycocalyx known as?

slime layer

Name 4 functions of capsule glycocalyx.

1. escape phytocytosis


2. energy source (as needed)


3. prevents loss of nutrients and water


4. attachment to tissues



When all flagella work together in unison, the prokaryote is:

running

When there is no uniform movement of flagella, which is attempting to change direction, the prokaryote is:

tumbling

Bacteria _______ toward positive stimuli.

run

Bacteria _________ from negative stimuli.

tumble

When there is a single flagella at one end, it is called:

monotrichous

When there is a tuft of flagella at one or both ends, it is called:

Amphitrichous

When there are more than 2 flagella at one or both ends, but usually just one end, it is:

Lophotrichous

When there are several flagella all over the bacterial cell, it is:

Peritrichous

What is the small, hair-like structure found all over a bacteria, and numbering in the hundreds?

Fimbriae

What is the purpose of fimbriae?

attachment

What is the purpose of pili?

Transfer genetic material from donor cell to recipient cell

How man pili does a cell have?

1 or 2

What is plasma membrane made of?

phospholipids and proteins

Except for Mycoplasma, what is not found in the plasma membrane of prokaryotes?

sterols

In the plasma membrane, what do the phospholipids form?

lipid bilayer

What are the 2 proteins found in the plasma membrane?

peripheral, integral

Which protein in the plasma membrane provides support in a scaffold manner?

Peripheral

Which protein in the plasma membrane forms a channel across the lipid bilayer, allowing passage of certain molecules in and out?

Integral

The arrangement of phospholipids and proteins in the plasma membrane that allows integral proteins to do their job without harming the structure of the membrane is known as:

Fluid-mosaic model

What is the substance of a prokaryotic cell inside of the plasma membrane?

Cytoplasm

What are the 3 major structures found in the cytoplasm?

nucleoid, ribosomes, inclusions

In addition to a single DNA chromosome, there are small genetic elements that can self replicate known as:

Plasmids

Are plasmids dependent on chromosome?

No

How many (noncrucial) genes does a plasmid carry?

5-100

Where in the cytoplasm is protein synthesized?

Ribosomes

Where in the cytoplasm are the reserve deposites, which usually store fat?

Inclusions

Endospores take place only in ____________ bacteria.

Gram+

What 2 bacterial species are guaranteed to make endospores?

Bacillus spp. and Clostridium spp.

What kind of environment are endospores created?

Adverse, by stressed bacteria

The process of endospore formation in a vegetative cell is known as:

sporulation

When an endospore returns to its vegetative cell, it is known as:

germination

What is inside of an endospore?

DNA of vegetative cell and proteins to get by