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15 Cards in this Set
- Front
- Back
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gram positive
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color will be purple /more sensitive to soaps & antibiotics
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gram negative
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color will be red/ more resistant to soaps & antiobiotics
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name the 5 I's of culturing microbes
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1.inoculation
2. isolation 3. incubation 4. inspection 5. indentification |
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inoculation
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introduction of a sample into a container of media to produce a culture of observable growth
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isolation
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separtating one species from another called streak plate method and isolate a single colony
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incubation
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under conditions that allow growth
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what is colonies
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visible growth of single bacteria
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how many species does a colony consist of
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one species
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what are the 3 properties media can be classified
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1.physical state
2. chemical composition 3. functional type |
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physical state
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liquid/semisolid/solid
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chemical compostition
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synthetic(chemically definded)/nonsynthetic(complex)
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functional type
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general purpose/enriched/selective/differential
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what is the most commonly used solidifying agent
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agar
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what is agar
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a complex polysaccharide isolated from red algae
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what are the properties of agar
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solid at room temp,liquefies at 100 degrees c
provides framework to hold moisture & nutrients *not digestible for most microbes |
