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11 Cards in this Set
- Front
- Back
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Seperation techniques are used in what type tests?
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Heterozygous Immunoassays
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What 2 separation techniques are used in Immunochemistry?
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Electrophoresis
Chromatography |
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What technique does electrophoresis use to separate substances?
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Using electrical current to separate pos & neg charges
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What 5 components are in electrophoresis?
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support media (agar)
chamber buffer electrodes power supply |
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What does the buffer provide?
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regulates temp, pH amnd ionic strength
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What measures the results of electrophoresis?
What factors can affect electrophoresis? |
densitometer
factors: voltage, current, time, buffer accuracy |
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What technique is used in chromatography?
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using a moving phase and a stationary phase to separate substances
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Give 3 examples of both a MP and a SP in chromatopgrophy:
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SP: paper, polymers, cellulose acetate
MP: liguid, gas, helium, soutions |
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Define Rentention Time:
Calculate Retention Factor: Define Rentention Volume: |
RT: time subs to get to endpoint
RF: subst. time to endopiont/ MP to endpoint RV: volume of MP needed |
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What is the resolution of a chromatographer
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ability to make distinct peaks
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Name the 4 types of chromatographers:
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Column (standard)
Thin layer (beaker) HPLC Gas (organic solvent) |
