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15 Cards in this Set
- Front
- Back
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give some basic information about process staining
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-the molecules of this dye or stain bind to different structures
-most stains are basic,that is +lycharged which enables them to bind to the bacteria which are negatively charged |
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what are the 2 types of stains?
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simple stain and differential stain
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what is simple staining intale?
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-uses only one dye
-it shows the size,the shape and the cellular arrangement |
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what is differential stains?
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-in which two or more dyes are used
-distunguishes between 2 kinds of organisms |
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what is gram stain?
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its part of the differential staining. three steps to gram staining...
-it uses crystal violet(1st stain, then iodine (2nd stain),mordant and stain it again w/counterstain (3rd stain-safranin) |
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what does mordant mean in the gram staining?
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a chemical used to retain stain in certain cells, a decolorizer
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what is a gram (G+) organism
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these organisms retain the violet stain in presence fo a decolorizer.
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what is a gram (G-) organism
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these organisms when decolorized, they lose their crystal violet stain color,now they don't have any color so when they are stained with the safranin dye counterstain)they stain red.
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what happens when a stain is called gram nonreactive?
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no stain or poorly
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what happens to a gram varible
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stain unevenly
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what happens in acid stain
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-dye is retained by some organisms and not by others
-the ones that it is called acid fast |
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what are the special stains (differential stains)
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-flagellar stain
-spore stain -negative stain |
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flagellar stain
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do not stain very well- difficult stain-stain flagella
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spore stain
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in rod shape bacteria-force bacteria to form spores- stain endospores
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negative stain
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these stain the background
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