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9 Cards in this Set

  • Front
  • Back

The final product of semiconservative replication:

each dsDNA helix contains one parent strand and one new daughter strand.

What hold the DNA strand togeather

Hydrogen bond

enzyme unwind the DNA helix

Topoisomerase

enzyme that unzipping the DNA

Helicase

enzyme synthesizing the RNA polymerase primer: nucleotides to the DNA chain.

Primase

enzyme the adds DNA nucleotides to the DNA chain

DNA Polymerase III

enzyme removes the RNA primer

DNA Polymerase I

the enzyme that seals the covalent sugar phosphate bonds in the DNA backbone

Ligase

Steps in DNA replication

a. at the replication origin, RNA primers are inserted



b. Strand separate and DNA polymerase III attaches adding the complimentary bases moving in the 5' to 3' direction



c. the lagging strand orients 3' to 5' and must be made backwards in short sections, 5' to 3' called Okazaki fragments



d. Okazaki fragments will be connected by ligase, after DNA polymerase removes the RNA primers and inserts the correct bases