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Photosynthesis

Conversion of light E into chemical E (sugar). Has 2 parts, Light Reaction & thr Calvin Cycle.



Needs CO2, H2O and sunlight E. Redox (reduction of CO2 & oxidation of H2O) reaction.



Takes place in chloroplasts in eukaryotes

Organisms who do photosynthesis

Cyanobacteria, algae, & plants

Organisms who use chloroplasts

Algae & plants

Over veiw of photosynthesis

6 carbon dioxide molecules + 6 water molecules --> 1 6 carbon sugar + 6 oxygen molecules (from H2O as waste product)



6 C SUGAR ALWAYS PRODUCED FROM CALVIN CYCLE & ALWAYS BREAKS IN HALF TO TWO 3C SUGARS

CO2 reduction

CO2 is reduced (gains electron & energy) into a sugar

H2O Oxidation

H2O oxidizes (loses electrons & energy) & gives them to CO2.

Electron transport chain

H2O oxidizes and gives its electrons to CO2 (causing reduction)

Light reaction

Sunlight E (absorbed by chlorophyll) enables water electrons to leave water and join carbon dioxide (electron transport chain). Electron movement used to produce ATP & NADPH. O2 produced at this point from water

Calvin cycle

Needs per cycle:


1 CO2


1 RuBP


1 Rubisco


2 NADPH


2 ATP



Forms per 3 cycles (1RuBP can process 2 CO2):


1 6-C sugar --> 2 3-C sugars ( glyceraldehyde 3-phosphate).



Sugar used in cellular respiration or makes organic molecules (lipids, amino acids, etc)



3 step process:


1. CO2 & RuBP combine in Rubisco's active site. 6-C sugar forms (ustable), immediately breaks into 2 3-C sugars (3-phosphoglycerate/3PG).



2. H+ & 2 e- added by 1 NADPH and Phosphate group added by 1 ATP to each 3PG (so 2 ATP & 2 NADPH per 1 CO2) to form glyceraldehyde 3-phosphate (G3P).



3. RuBP recharged for next CO2. Need 5 G3P 9 ATP 6 NADPH to regenerate 3 RuBP. 3 RuBP makes 6 G3P

Thylakoid membrane

In chloroplast. Electron transport chain and chlorophyll pigment located here. Light reactions happens here.

Stroma

Colorless fluid in chloroplast, surrounds the Thylakoid membrane. The calvin cycle takes place here.

Light

Different wavelengths = different colours.



When it hits matter, it's:


Reflected (colour you see)


Transmitted (goes thru)


Absorbed

Pigment

Molecule that absorbs certain wavelengths (colours).

Chlorophyll

Absorbs blue & red.


Reflects/transmits green

Carotenoids

Absorbs blue & green



Reflects yellow & orange

RuBP

Ribulose bis phosphate.


Start/end molecule in Calvin cycle. Is a 5-C sugar.

Rubisco

Enzyme that binds RuBP & CO2 in calvin cycle

Challenges to photosynthetic efficiency

1. Too much light E


2. Photorespiration

2 things

Too much light E & 2 solutions

Plant can't process e- fast enough. e- combines w O2 (forms free radicals) and damages plant molecules.



Solution:


1. Pigments absorb E from Chlorophyll & release it as heat


3. Detoxifies reactive molecules w anti-oxidants (like vitamin C)

Photorespiration

Occurs when low [CO2] and high [O2] in cell. Enzyme rubisco accidentally accepts an O2 instead of CO2 into active site. Then binds O2 to RuBP --> RuBP breaks apart & is lost. Also no sugar made.



Can lose up to 50% of sugars this way.

How did photorespiration occur?

Calvin Cycle/Photosynthesis evolved when there was little/no O2 in atmosphere/ocean.



Has not evolved a better way yet

How much solar E that hits a leaf is converted into carbohydrates/sugar?

1-2%

NADP+ + 2e- + H+ -->

NADPH

ADP + H+ + phosphate --->

ATP. Proton/H+ used to glue phosphate group to ADP

How do pigments absorb E?

Absorb E by having e- bumped up to a higher E shell. E always stored in electrons as potential E

When is photorespiration at its worst

Worse on hot/sunny days. Leaf closes stomata (leaf pore) to save water and traps O2 as well.

How do plants not run out of e- during photosynthesis?

e- taken from H+ in H2O. So long as plant has new H2O it will never run out of e-