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37 Cards in this Set

  • Front
  • Back

Acute phase

Time to collect the specimen

Cotton, Dacron or Calcium alginate

The tip of the swabs may contain

Clean catch midstream

Method used in collecting urine

Etiologic agents

Microorganism causing disease

Three separate early morning specimens

Method for sputum in fungal and mycobacterial infections

3 specimens: Once a day for 3 days

Collection of stool for bacterial infection

3 specimens collected within 10 days

Collection of stool for parasitic infection

4-5 days

Days needed to be delayed when barium studies are done

30 minutes; 2 hours

Specimens should be transported to the laboratory ideally within _____, preferqbly within ______

Ref temperature 4C for 24 hours

Specimen storage for urine, stool, sputum, swabs, catheters

35C for 6 hours

Specimen storage for CSF that cannot yet be processed

Boric acid

Used to maintain accurate urine colony counts

Cary-Blair transport media

Stool specimens that is delayed longer than 2 hours

Frozen at -70C

Stools for Clostridium difficile toxin that is delayed longer than 48 hours

Refrigerate

CSF for viruses

Room temp

CSF for bacteria

Room temp

Preserved Feces

Refrigerate

Unpreserved feces

SPS

Most commonly used anticoagulant for microbiology specimens

0.025%

The conc of SPS must not exceed at

Heparin

Used for viral and mycobacterium spp.

JEMBEC system

Transport media or transport system for N. gonorrhoeae

Every 2 years

Training if shipping is by air

Every 3 years

Training if shipping is by land or ground

Level 3

Level for quantitation

Level 4

Level for preserved specimens

Level 1

Level for potentially life threatening illness

Level 2

Unpreserved specimens and may quickly degrade or have overgrowth of contaminating flora

Streptococcus pneumoniae

Gram positive diplococci

3000xg for 20 minutes

If the specimen is greater than 1ml, centrifuge at

Lim broth or Todd Hewitt with CNA

Used to enhance the growth of group B strep

35°C -37°C

Most bacterial cultures are incubated at

48-72 hours

Most routine bacterial cultures are held at

5-7 days

Cultures for anaerobes and broth cultures may be held for

Skirrow

Media for H. pylori

Loeffler agar slant

Media for F.tularensis

Regan-Lowe or Bordet Gengou

Media for B.pertussis