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40 Cards in this Set

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Define:
Taxis.
Positive phototaxis.
Negative phototaxis.

Taxis: directed movement toward or away from a stimulus.
Positive phototaxis: movement TOWARD light


Negative phototaxis: movement AWAY FROM light

Taxis go places.

Define:
Endospore

A thick-coated, resistant cell produced by some bacterial cells when they are exposed to harsh conditions. (when lack an essential nutrient)

The original cell produces a copy of its chromosome and surrounds that copy with a tough multilayered structure forming the endospore.

endo, encase, enhance


when exposed

Define:
Binary fission

Method of asexual reproduction by "division in half."

In prokaryotes, no mitoses involved, but in single-celled eukaryotes mitosis is involved.

Define:
Phototroph

Organisms that obtain energy from the light

Define:
Chemotroph

Organisms that obtain energy from chemicals

Define:
Autotroph

Organisms that need only CO2 or related compounds as a carbon source

Define:
Heterotroph

Organisms that require at least one organic nutrient (i.e. glucose) to make other organic compounds

Define:
Obligate Anaerobe

Organism that carries out only fermentation or anaerobic respiration. They can NOT use oxygen and are poisoned by it.

obligated to be anaerobic, anti air

Define:
Obligate Aerobe

Organism that requires oxygen for cellular respiration and to survive.

obligated to air, aerobic

Define:
Facultative Anaerobe

Organism that makes ATP by aerobic respiration if oxygen is present but SWITCHES to anaerobic respiration or fermentation if oxygen is not present. (so both)

facultative: capable but not restricted to something/function

Define:
Bioremediation

The use of organisms to detoxify and restore polluted and degraded ecosystems.

bio-life,organisms


remedi --> remedy




organisms remedy, restore

What are the two main branches of prokaryotic evolution?

Bacteria

Archaea

NOT eukaryotic. No nucleus.

What are the components of a prokaryotic cell wall?

Bacterial: peptidoglycan: sugars x-linked with polypeptides




Achaea: contain polysaccharides (carbs) and proteins but NO peptidoclycan

one has sugars, the other does not and instead has carbs and proteins

Define:
Peptidoglycan

Sugars x-linked with polypeptides

pepti --> pepsi .... sugar
glycan ....sugar

Function of cell walls
(nearly all prokaryotes)

1: maintain shape
2: physical protection
3: prevents bursting in hypotonic environment (will plasmolyze in hypertonic environement)

Hypotonic vs hypertonic

REVIEW THIS

Hypotonic: lower concentration of solutes outside the cell than inside the cell; solute enters cell, may burst
 Hypertonic: higher concentration of solute outside cell

Hypotonic: lower concentration of solutes outside the cell than inside the cell; solute enters cell, may burst


Hypertonic:


higher concentration of solute outside cell

Difference between gram positive and gram negative?

bacteria

Gram positive: simpler but thick walls, large amount of peptidoglycan (sugar x-linked), shows up when stained bc crystal violet can't pass through THICK WALL, shows up violet over red safranin

Gram negative: less peptidoglycan, structurally more complex, outer membrane contains lipopolysaccharides (carbs/lipids); allows violet dye to wash through bc THIN WALLS so shows up red/safranin

Why are Gram negative pathogens generally more threatening than Gram positive ones?

Among Pathogens - gramnegative more threatening than gram positive


(1) Lipopolysaccharide- toxic




(2) Outermembrane protects against host defenses




(3) Greaterresistance to antibiotics

3 reasons

How does penicillin inhibit prokaryotic growth?

Penicillin inhibits peptidoglycan x-linking. May make cell wall not functional, esp in gram positive bacteria.

penicillin does not like sugar formations

What is a capsule and what is its function?

many prokaryotes

Sticky layer of polysaccharide (carbs) or protein that surrounds the cell wall; dense and well-defined (if not then is slime layer)




FUNCTION:


1.Enableprokaryotes to adhere to their substrate or to other individuals to form acolony


2. Can protect against dehydration or shieldpathogenic species from host’s immune system




Some protect from host's immune system, SHIELD

outside of cell wall

What are fimbriae and what do they do?

some prokaryotes

Hairlike appendages allow to stick to substrate or one another

fim-BRAID, HAIR-like

What are the major differences between prokaryotes and eukaryotes?

Prokaryotes do NOT have nucleus (have nucleoid), eukaryotes HAVE nucleus.

Prokaryotes:
(1) Lack compartmentalization by internal membranes


(2) Many have infolded regions of the plasma membrane


(3) Smaller simpler genomes ~1/1000 as much DNA double-strandedcircular DNA with many fewer associatedproteins than eukaryotes mayhave smaller rings of DNA - plasmids neededfor special contingencies replicateindependently canbe transferred between partners


(4) Ribosomes smaller & differ in protein and RNA content



Define:
Flagellum,
Flagella

Thin thread-like structure that allows movement/ propulsion




Prokaryotes and eukaryotes are composed of different things, but perform similar functions

a flag waves and MOVES in the wind

What is a plasmid and what is its function?

prokaryotes

Small rings of independently replicating DNA molecules, most carrying only a few genes

How do the antibiotics erythromycin and tetracycline inhibit bacterial growth?
Certain antibiotics such as erythromycinand tetracycline bindto prokaryotic ribosomes and block protein synthesis.

Define:
Ribosomes

A minute particle consisting of RNA and associated proteins, found in large numbers in the cytoplasm of living cells. They bind messenger RNA and transfer RNA to synthesize polypeptides and proteins.

Define:
Horizontal gene transfer

Bringing together DNA from individuals ofdifferent species; facilitates rapid evolution

Transfer of genes from one genome to another through mechanisms such as transposable elements, plasmid exchange, viral activity, and perhaps fusions of different organisms

evolutionary trees

What are the three mechanisms prokaryotes use to transfer genes between individuals?

(1) Transformation- cell takes up genes from surrounding environment


(2) Transduction - viruses transfer genes fromone prokaryote to another


(3) Conjugation- direct transfer of genes from one prokaryote to another

Distinguish the following four major modes of nutrition observed in prokaryotes,photoautotrophs, chemoautotrophs, photoheterotrophs, chemoheterotrophs.

Can be grouped into 4 categoriesaccording to energy & carbon use(1) Phototroph -use light energy(2) Chemotroph-energy from chemicals in environment(3) Autotroph-need only CO2 (inorganic)(4) Heterotroph-require at least one organic nutrientCombining the above leads to 4 majormodes of nutrition>

may need additional info

How does denitrification differ from nitrogen fixation?

Nitrogen fixation -convert N2 to ammonia NH3 onlybiomechanism that makes atmospheric N2 available to organisms

ADD MORE INFO

What advantage does metabolic cooperation confer?

Cooperation between prokaryotes allowsthem to use environmental resources they could not use as individual cells.

Define:
Biofilm

Surface-coating where metabolic cooperation between different prokaryotic species often occurs

Cells in a biofilm secrete signaling molecules that recruit nearby cells, causing colonies to grow.

film, on the surface

What is an extreme thermophile? halophile? methanogen?

(1) ExtremeThermophiles - thrive in hot environments60 - >90° C


(2) Extreme Halophiles - live in high-salt environments - Great Salt Lake, Dead Sea


(3) Methanogens obtainenergy by using CO2 to oxidize H2 CH4 strict anaerobes - poisoned by O2

phile = lover


lover of extremes

What is meant by the term symbiosis? List the three types of symbiotic relationships.

"Living together"; An ecological relationship between organisms of different species thatare in direct contact.




(1) Mutualism -both benefit(2)Commensalism -one receives benefits while neitherharming nor helping the other (i.e. Carrier Crab) (3)Parasitism -one (parasite) benefits at expense ofthe host

Define:
pathogens

parasites that cause disease

What is an opportunistic pathogen?

Pathogens that take advantage of OPPORTUNITIES not usually available, such as a host with a weakened immune system

How many diseases in humans do pathogenic prokaryotes cause?

Half of all human diseases

What is the difference between an endotoxin and exotoxin?

Endotoxin: lipopolysaccharide components of the outer membranes of certain gram-negative bacteria

Exotoxin: proteinssecreted by prokaryote


1 gram of this prokaryotes exoTOXIN can kill 1,000,000 people ( 1g - kill 1x10^6)

botulism

The evolution of antibiotic resistant strains of bacteria is on the rise. Explain why?
Exacerbated by imprudent antibiotic useand horizontal gene transfer