Chapter 20

Front 1

. Human genes/proteins that regulate the cell cycle are most easily isolated by

Back 1

complementation of yeast mutants

Front 2

What is a target of the APC

Back 2

cyclin B

Front 3

How do chromosome movements differ between meiosis I and meiosis II? Is spindle composition the basis for this distinction?

Back 3

During meiosis I, synapsed homologous chromosomes are segregated to the two daughter cells. During meiosis II, sister chromatids are segregated as in a mitotic division. Factors associated with the chromosomes rather than the spindles provide the basis for this distinction, because a meiosis I spindle is capable of segregating meiosis II chromosomes properly, and a meiosis II spindle will segregate meiosis I chromosomes properly.

Front 4

Phosphorylation of Thr-161 on S. pombe MPF

Back 4

is required for MPF activity

Front 5

What S. pombe mutants is larger than normal?

Back 5

cdc25-

Front 6

Exit from mitosis depends upon the degradation of

Back 6

cyclin B.

Front 7

Separase initiates sister chromatid segregation at anaphase by cleaving

Back 7

Scc1(kleisin)

Front 8

Cyclin D/Cdk4 functions during

Back 8

G1

Front 9

Name three activities of G1 cyclin-CDKs that ensure progression through the cell cycle

Back 9

G1 cyclin-CDKs inactivate the APC, activate expression of S-phase cyclins, and phosphorylate the S-phase inhibitor, which targets it for degradation by the SCF.

Front 10

Centromeric Rec8 becomes cleaved during

Back 10

meiosis II