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19 Cards in this Set
- Front
- Back
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TH1 v TH2
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TH1 involved in intracellular microbe invasion
TH2 involved in intracellular helminthes differentiation is determined by cytokines in microenvironment; link of innate to adaptive |
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TH1
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Diff signal: IFN-g
IL-12 (from MQ) Secretes: IFN-g JAK/STAT pathway txt factors: (Tbet, STAT1) --> (STAT4) Tbet is the master regulator. involves chromatin remodeling |
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TH2
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Diff signal: IL-4
Secrete: IL-4, IL-10 txt factors (GATA-3, STAT6) --> (STAT6, GATA-3) |
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IL-12 and IFN-g Feedback loop
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IL-12 produced by APCs, particularly MQ
NK produce IFN-g (and TH1) IFN-g --> MQ --> IL-12 --> TH1 -->IFN-g |
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TH1 differentiation pathway
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using JAK/STAT pathway
JAK: recruiting kinase for STAT STAT: once dimerizes, can enter nucleus and initiate txt for differentiation |
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TH1 effector functions
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All using IFN-g
Stim Phagocytosis (MQ, DC) Stim Opsonization (via isotype switching) Stim Complement fixing (via Ab) Using LT: stim Neutrophils to prod more granules Using TNF: stim upregulation of selectin/integrin on endothelial |
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Selectin and Integrin placement
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Selectin receptor and Integrin ligand on endothelial cell
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IFN-g
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Stimulates:
istoype switching dif into TH1 cells (via JAK/STAT) MQ --> more IL-12 MQ --> more phagocytosis Secreted by: CD4+ NK |
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IL-12
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Secreted by:
mononuclear phagocytes DC Stimulates: NK activation Promotes IFN-g prod by NK and CD4+ diff CD4+ --> TH1 |
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IL-2
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Secreted by:
CD4+ Stimulates: Proliferation of T cells , NK, B cells |
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IL-10
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Secreted by:
MQ some T cells Stimulate: inhibition of innate and cell-mediated (B and T) |
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IL-4
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Secreted by:
TH2 Stimulates: Isotype switching for IgE stim diff of TH2 cells |
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CXCR3, CCR5
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are on TH1 surface (not TH2 at all)
conferred by txt master regulator Tbe These bind chemokines to direct TH1 to site of inflammation |
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CCR4
CCR8 CXCR4 |
These are on the surface of TH2 cells and direct them to site of helminth infection
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IL-5
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Secreted:
TH2 Mast Cells Stimulates: diff of eosinophils |
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MQ activation by TH1
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TH1 secretes IFN-g and w/ CD40:CD40L
stim MQ to do more work phagocytosis IL-12 secretion also Stimulates signaling casades for txt factors in orderto produce more necessary proteins (NF-KB, AP-1) |
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Phagocytosis by???
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Not granzymes or perforin ! (THat is for CTLs and NK)
Use Phagocyte oxidase --> ROS iNOS --> No2 and lysozymes |
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TGF-B
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Secreted by:
T cells Phagocytes Stimulates: Inhibition of T cell diff MQ activation counteract inflammatory cytokines |
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TNF
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Secreted by:
Phagocytes that recruit neutrophils Stimulates: endotehlial upregulation of adhesion molecules MQ and Endothelial to secrete chemokines Promotes apoptosis of targets |
