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12 Cards in this Set
- Front
- Back
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1. Which gene is incorporated into plasmids to detect recombinant cells? |
A gene for antibiotic resistance |
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2. Which of the following is NOT essential to carry out the polymerase chain reaction? |
Primers DNA polymerase Gel electrophoresis High temperature |
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3. Which of the following is NOT a part of the Sanger method to sequence DNA? |
Dideoxynucleotides DNA polymerase Electrophoresis Reverse transcriptase |
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4. The function of ligase is to |
Rejoin segments of DNA |
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5. The pathogen of plant roots that is used as a cloning host is |
Agrobacterium |
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6. Which of the following sequences, when combined with its complement, would be clipped by a restriction endonuclease? |
GAATTC |
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7. Which DNA fragment will be closest to the top (negative pole) of an electrophoretic gel? |
5 kb |
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8. Which of the following is or are required for a cloning at gene? |
Restriction endonuclease Vector Cloning host (All of these) |
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9. For which of the following would a nucleate acid probe NOT be used? |
Locating a gene on a chromosome Developing a Southern blot Identifying a microorganism Constructing a recombinant plasmid |
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Technique that separates a readable pattern of DNA fragments is |
Gel electrophoresis |
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Which PCR step synthesizes complementary DNA strands? |
Add DNA polymerase and nucleotides at 72°C |
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Which of the following is a serious concern when performing PCR? |
Introduction and amplification of contaminating DNA |
