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11 Cards in this Set
- Front
- Back
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techniques deliberately remove genetic material from one organism and combine it with the genetic material of a different organism |
Recombinant DNA |
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What are the 5 steps of the recombinant DNA process |
1) Isolate a desired gene 2) Insert gene into vector 3) Transfer the vector to a cell 4) Clone the cell containing the vector 5) Harvest gene and isolate the product |
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What are genomic libraries |
Genes maintained in a cloning host or vector, just like a pure culture |
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In genomic libraries their are collections of _____ _____ that represent the entire genome of numerous organism |
cDNA clones |
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(such as plasmids and bacterophages) should have three important attributes: |
Cloning Vectors;
1)An origin of replication somewhere on the vector 2) Must accept DNA of the desired size 3)Contain a gene that confers drug resistance to their cloning host |
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Traditional host, but lacks versatility in correctly expressing eukaryotic genes |
E. coli |
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____________ _________, which fulfills most of the criteria, is widely used in industry; eukaryotic gene expression |
Saccaromyces cerevisiae |
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What gene was spliced into E. coli plasmid? |
interferon gene |
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How was the interferon gene spliced into E. coli |
1) plasmids circular molecule was nicked open with an endonuclease and the sticky ends were exposed.
2) the free ends are based paired
3) Ligase made the final covalent seal |
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Plasmid combination was introduced by _____________ into the cloning host |
screening test |
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The plasmid with gene of interest - the interferon gene, also carries a __________________
Clones then were streaked onto media containing _________, and only those that carried the plasmid survived |
gene for ampicillin resistance |
