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8 Cards in this Set
- Front
- Back
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what does shearing do
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protein unfolding, refolding, in a non native form
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what does shaking do
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causes foaming or an increase in the air-liquid interface and oxidation
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what are the analytical techniques
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SDS-PAGE
Immunoblotting ELISA Chromatography |
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what is SDS-Page used for
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determine the molecular weight of proteins
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what occurs in SDS-Page
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proteins are suspended in dye containing B-ME or DTT to reduce disulfide bridges
proteins are denatured by SDS which confers a negative charge to the polypeptide proteins migrate based on molecular weight |
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what is Western blotting or immunoblotting for
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to determine the relative amounts of protein present in different samples
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what is Southern blotting used for
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identification of DNA sequences
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what are examples of buffer systems
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phosphate
citrate acetate |
