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5 Cards in this Set
- Front
- Back
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What can be used to track cells? |
Vital stains - charcoal & Nile blue |
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Genetic methods of marking a cell |
1. Transformation (DNA in solution) 2. Transduction (via a virus) 3. Electroporation (via current) 4. Sonoporation (via sound) 5. Gene gun (bombard cells) 6. Microinjection 7. Gene expression markers (eg. GFP) 8. CRE-Lox recombination 9. Labelling multiple cells (eg. the "Brainbow" system) |
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What are the gene expression markers that can be used? |
# Green Fluorescent Protein (GFP);
# Escherichia coli - β-galactosidase (LacZ gene) # LGR5+ protein [Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)] |
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What is CRE recombination? |
Cre-Lox recombination -
a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells.
It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus.
It is implemented both in eukaryotic and prokaryotic systems. Particularly used in neuroscience. |
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What is a "Brainbow", and how is it used? |
Brainbow - is a process by which individual neurons in the brain can be distinguished from neighboring neurons using fluorescent proteins. This technique has been a major contribution to the study of neural connections. |
