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15 Cards in this Set

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Advantages of light microscope

Colour images produced


Observe living cells

Disadvantages of light microscopes

Long light wavelength means lower resolution

0.2um max resolution

Advantages of electron microscope

Electrons have a short wavelength so greater resolving power


Electrons are negatively charged so can be focused by an electromagnet

Max resolution 0.1nm

Disadvantages of electron microscopes

Sample needs to be in a vacuum so only dead organisms can be observed


Black and white images produced


Due to preparation method artefacts may be produced

What is the definition of resolution?

The ability to distinguish between two separate points

Advantages of TEM

Highest resolution

Disadvantages of TEM

Can't observe living organism as it's in vacuum


Complex preparation method


Thin specimens only


May contain artefacts

Advantages of SEM

Sample does not need to be as thin as SEM


3D images produces

Disadvantages of SEM

Lower resolving power than TEM

What is the function of the nucleus?

Controls cell activities


Contains DNA


Controls production of mRNA tRNA ribosomes and protein synthesis

Nuclear envelope

Double membrane


Controls entry and exit of substance into nucleus

Nuclear pores

Allows large molecules such as mRNA out of nucleus

Nucleoplasm

Jelly like cytoplasm of nucleus

Chromosomes

Consists of DNA associated with histones

Nucleolus

Manufactures RNA and assembles ribomes