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76 Cards in this Set

  • Front
  • Back
What are the primary functions of membranes surrounding the cell and organelles?
To separate compartments and to control molecular traffic in and out.
Membranes are selectively permeable. Define what this means.
Some substances can cross easily while others can not get across.
Membranes are made up of what two things?
50% Phospholipids, and 50%Proteins.
What does "fluid" membrane refer to?
It is constantly moving. The phospholipids are freely moving and the proteins float freely in the lipids.
The fluidity of the membrane is impacted by what?
Level of saturation and Temperature (colder slower, and warmer faster).
Where does saturated and unsaturated fat come from?
Saturated fat comes from animals, and Unsaturated fat comes from plants.
What is the importance of Steroid Cholesterol?
The cholesterol keeps cells from freezing by "breaking up" the lipids at low temperatures.
Proteins of a membrane can be Peripheral and Integral. Explain the difference.
Peripheral proteins are anchored to a phospholipid and move with the phospholipid in one layer (does not cross bilayer). Integral protein goes from one side of the membrane to the other, it must also have a region that has hydrophobic amino acids (completely crosses bilayer).
What are the six functions of membrane proteins? Then explain each.
Transporters (materials in and out of the cell), enzymes (facilitate chemical reactions), cell surface receptors (transmit messages from outside cell), cell surface identity markers (marker to let other cells know the cell type), cell to cell adhesion proteins (support cell and surrounding tissue), and attachments to the cytoskeleton (support cell and surrounding tissue).
Membrane proteins and lipids have what in common?
Synthesized in the ER and Golgi and transported out in vesicles.
Membranes are Bifacial what does this mean and why?
Have a cytoplasmic inside and outside. Proteins have to be oriented correctly to function, also sugar groups can be attached to the outside.
What two molecules can diffuse directly across the lipid bilayer?
Apolar molecules, and small uncharged polar molecules.
Larger polar molecules and ions must use what to get across the membrane?
Transport proteins.
The passive transport of water across the membrane is called what? Explain.
Osmosis. The water will always travel to where there is more solutes (sugar, salt), and less water. If the solutes can not cross the membrane then the water will move to make the concentration equal.
Define Hypertonic, Hypotonic, and Isotonic.
Hypertonic is less water and more solutes, Hypotonic is more water and less solutes, and Isotonic is equal.
Which solution of osmosis do plant and animla cells perfer?
Plant cells perfer Hypotonic, and Animal cells perfer Isotonic.
What are the three types of membrane transport? Desribe each.
Passive Transport (with or without a protein, also known as diffusion. No energy required moves from high to low concentrations) Active Transport (must have a protein, requires energy, goes against concentration gradient from low to high), and Facilitated Transport (another form of diffusion it makes use of a transporter protein through the use of its channel)
What is a Channel Protein?
A protein that allows fast transport of molecules in and out of a cell when their channels or "gates" are open
What is the difference between diffusion and facilitated diffusion?
Some of the protein channels can be closed. Allow for a gradient bulid up and then a rush of the molecule when needed.
One example of an active transport is a protein pump. Explain a protein pump.
Moves protons outside of the cell against their concentration gradient. The movement of protons creates a membrane potential and an electrochemical gradient.
Carrier Proteins used in Active Transport include what?
Uniporters (move one molecule at a time), Symporters (move two molecules in the same direction), and Antiporters (Move two molecules in opposite directions, pull protein out and something out with it, doesn't require ATP)
What is the Sodium-Potassium Pump?
An active transport mechanism that uses an antiporter to move 3 NA+ out of the cell and 2 K- into the cell. Basically swaps them.
Bulk Transport of substances is accomplished by what five things?
Endocytosis (movement of substances into the cell), Phagocytosis (the cell takes in particulate matter), Pinocytosis (The cell takes in only fluid), Receptor-Mediated Endocytosis (Specific molecules are taken in after they bind to a receptor), and Exocytosis (movement of materials out of the cell).
What is Metabolism?
The sum of all biochemical reactions in the cell.
What are the two types of metabolism?
Anabolism (synthesis reactions or building something), Catabolism (decomposition reactions or breaking something down).
What is energy?
Energy is the capacity to do work.
What are the six types of energy?
Potential Energy, Kinetic Energy, Chemical Energy (energy in bonds such as ATP), Radiation Energy (light), Nuclear Energy (nucleus), and Heat (kinetic energy of molcules).
Most forms of energy can be converted into heat energy. Heat energy is measured in kilocalories. Define one calorie.
The amount of heat required to raise the temp. of 1kg of whater by 1 degree C. 1 food calorie= 1 kilocalorie= 1000 calories.
What is the first law of thermodynamics?
It is the law of energy conservation. Any form of energy can be transformed to other forms of energy, but energy is never lost or created.
What is the second law of thermodynamics?
Disorder is more likely than order. Entropy (disorder in the universe), entropy is always increasing.
Chemical reactions can be described by the transfer of energy. What are the two types of chemical reactions?
Endergonic Reaction (a reaction requiring an input of energy. Exergonic Reaction (a reaction that releases energy)
What is a catalysts?
Substances that lower the activation energy of a reactions.
What is the ATP structure?
Ribose (5-carbon sugar) + adenine + three phosphates
What are Enzymes?
Molecules that catalyze reactions in living cells. Most are proteins that lower the activation energy required for a reaction, and are not changed or consumed by the reaction.
Enzymes are specific, give examples.
They accelerate one particular reaction, The reactant(s), that are used are called the substrate, the spot where the two bind is the active site.
What four factors influence enzyme reaction rates?
Enzyme concentration, Substrate concentration, Environment, and Regulatory molecules.
Several regulatory molecules impact enzyme activity explain.
Inhibitors are molecules that bind to the enzymes and decrease activity, Competitive inhibitors compete with the substrate for binding to the same active site. Noncompetitive inhibitors bind to sites other than the enzymes active site. Also Activators are molecules that bind to the enzyme that increase activity, and Cofactors are non-protein molecules/atom required for enzyme activity.
Cellular Respiration occurs in and around the ____ to produce chemical energy.
Mitochondria.
Organisms can be classified based on how they obtain energy. What are the two classifications?
Autotrophs: are able to produce their own organic molecules through photosynthesis. (Plants) Heterotrophs: live on organic compounds produced by other organisms. (Animals)
The equation for cellular respiration is:
C6 H12 06 + 6o2 ---> 6CO2 + 6H2O + 38 ATP
Explain. C6 H12 06 is glucose, 602 is six oxygen, 6 CO2 is six carbon dioxide, 6H2O is six waters, and 38 ATP is 38 ATP.
What is ATP?
Adenosine-tri-phosphate, which is the main source of energy for cellular work.
What causes electronegativity?
Elements want to have a full outer shell, and as the electron circles the nucleus it has to be a greater distanace between shells to have high energy and not fall away from the nucleus.
Oxygen is very electronegative which gives it what two qualities?
It attracts electrons, and is a strong oxidizer.
NAD+ does what to become NADH?
Accepts 2 electrons and 1 proton, the reaction is reversible and used to used to move electrons in cellular respiration.
What is Aerobic Respiration?
Large amount of energy that must be released in small steps rather than all at once. (going down steps not jumping to the bottom).
The complete oxidation of glucose proceeds in stages list the 5 stages.
Glycolysis (splits), Pyruvate Oxidation, Krebs Cycle, Electron Transport Chain, and Themlosmosis.
Explain Glycolysis.
Occurs outside of the mitochondria, Splits the C6 sugar glucose into 2 C3 sugars called pyruvate in a ten step process of using 2 ATP and gives off 4 ATP and 2 NADH's which results in a net of 2 ATP and 2 NADH's.
In Pyruvate Oxidation what occurs.
The pyruvate is converted into Acetyl CoA by giving off a CO2 and gives a NADH.
What occurs in the Krebs Cycle?
The acetyle CoA enters the cycle and results in 2 CO2 given off, 3 NADH's produced, 1 ATP produced, and 1 FADH produced. (multiply by two per glucose)
What occurs in the Electron Transport Chain (ETC)?
The NADH and FADH give their electrons to the ETC, the electrons tumble down it losing energy as they go, this results in protons being pumped out of the inner mitochondrial membrane and creates a proton gradient, the electron is given to O2 at the end thus aerobic respiraiton.
In the ETC for each NADH and FADH that is pumped in what is pumped out?
NADH pumps out 3 protons, and FADH pumps out 2 protons.
What occurs at the Chemiosmosis stage?
The protons accumulate and are passed back over the membrane through the ATP synthase protein, For each proton that comes through ADP + P is changed into ATP.
What is the grand total of ATP produced per glucose?
38 ATP.
What are the theoretical energy yields of repiration, and then what are the acual energy yields?
Theoretical: 38 ATP per glucose for bacteria and 36 per glucose for eukaryotes. Actual: 30 ATP per glucose for eukaryotes, reduced yield is due to leaky inner membrane.
What two types of respiration occur without O2?
Anaerobic Respiration (use of inorganic molecules as final electron acceptor Sulfer or, Nitrogen), and Fermentation (use of organic molecules as final electron acceptor such as ethanol (plants), lactic acid (lactic acid)).
All sugars enter food chain via _______.
Photosynthesis.
Photosynthesis is carried out by which three groups?
Cyanobacteria (prokaryotes that under go photosynthesis and oxygen production), 7 groups of algae, and all land plants.
The make up of photosynthesis is 6 CO2 + 6 H2O ---> C6 H12 O6 + O2. (H+ + e-) taken from one side given to the other.
To make carbohydrates CO2 provides C, O., H20 provides H, e-., O2 is a byproduct., The increase of energy is provided by light e- in water: low energy, e- in sugar: high-energy.
Photosynthesis takes place in where?
Chloroplasts. The Thylakoid Membrane: the actual pancake things, and Stroma: the liquid in the organelle.
Photosynthesis is divided into what two reactions?
Light-Dependent Reactions: caputre energy from sunlight, make ATP and reduce NADP+ to NADPH, in thylakoid. Carbon Fixation Reaction: Use ATP and NADPH (from light reactions), located in the stroma.
Light is _____ from the sun.
Radiation separted by energy levels.
Visible light is either ____, _____, or ______ by any given surface.
Reflected, Transmitted, or Absorbed.
Pigments are able to absorb specific wavelengths of light, ______ is the main pigment in photosynthesis.
Chlorophyll, it is the green pigment (seen by the eye) in plants.
Chlorophyll mainly absorbs light in the _____ and ______ parts of the spectrum.
Blue and Red.
What is the purpose of a carotenoid?
Protect the plant from excess light.
Photon is a particle of light, a bundle of energy. When a molecule absorbs a photon one of the molecule's electrons is elevated to a higher electron shell giving it greater ______ ______.
Potential Energy.
Explain Photosystem II.
Absorbs photons and excites that are passed to plastoquinone.
Explain the b6f complex.
The b6f complex transfers the electrons from plastoquinone to plastocyanin. THe electron transfer provides energy to pump a proton over the thylakoid membrane.
Explain Photosystem I.
Absorbs photons and re-excites electrons that are passed through carriers and ultimately reduce NADP+ to NADPH.
Explain Step 4.
Protons on the inside of the thylakoid membrane flow back through the ATP synthase protein, converting ADP + P to ATP.
In the Carbon Fixation Reactions, in order to build carbohydrates cells need what?
Energy (ATP), Reduction Potential (electrons), A source of Carbon (CO2 from the air). This is the Calvin Cycle.
The Calvin Cycle has three Phases list them.
Carbon Fixation (RuBP (5 carbon sugar) + CO2 --> 2 molecules PGA (3 carbon sugar), Reduction, and Regeneration of RuBP (G3P is used to regenerate RuBP).
What is Rubisco?
The enzyme that fixes CO2 from the air, the most abundant enzyme in the world.
What is required to make one molecule of glucose?
2 G3P, 18 ATP molecules, and 12 NADPH molecules.
What are the two forms of alternate photosynthesis?
C4 metabolism, and Crassulacean Acid Metabolism (CAM).
What is Photoresperiation?
Occurs when plants only have rubisco, when rubisco binds with oxygen instead of CO2. Occurs when temp is high or too dry.