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21 Cards in this Set

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who observed cork and first named "cells" in 1665
robert hooke
what was the magnification power of the first microscope?
30x
Who produced the 300x light microscope?
antonie van leeuwenhoek
why was progress in early cell biology slow?
limited resolution and focus only on observation, not explanation
2 reasons
magnification power
how many times the size of the specimen is enlarged
resolution
ability to see fine details
what are compound microscopes and why were they important?
two lenses. increased magnification and resolution meaning structures 1 micrometer in size could be seen
who discovered the nucleus and where?
robert brown in plant cells
what is matthias schleiden known for?
all plant tissues are made of cells
who discovered all animal tissues are made of cells?
thomas schwann
in 1839, who came up with the first two statements of the cell theory?
schwann
who and when did the third part of the cell theory get added?
virchow in 1855
cell theory
1. all organisms consist of one or more cells. 2. the cell is the basic unit of life. 3. all cells come from preexisting cells
three strands of modern cell biology
cytology-observation of cell structures, biochemistry-cellular function at chemical level, genetics-information flow and heredity.
two classes of microscopes
light and electron
what are the main differences?
light uses glass lenses and seen by the naked eye. electron uses electromagnetic lenses and seen on fluorescent screen or photographic film
phase contrast microscopy
uses a refractive index different from the medium for color. best for contrast and live cells
fluorescence microscopy
detects fluorescent dyes/labels to locate certain substances in the cell. can be done on live cells.
differential interference contrast microscopy
enhanced resolution, shadow casting effect, good view of the surface of the specimen, good for live cells
limit of resolution
how far apart molecules must be from each other to be seen separately, greater resolving power=smaller limit of resolution
how is limit of resolution determined?
wavelength of the light used divided by 2.