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5 Cards in this Set
- Front
- Back
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List the 3 types of labeling
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1. Organic dyes
2. In situ hybridization 3. Antibodies |
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Organic dyes
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1. chemical affinity for specific cell component
(Ex. Hematocylin is acidophilic, meaning DNA, RNA, and acidic proteins) |
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Hematocylin
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Type of organic dye that is used in labeling DNA, RNA, and acidic proteins because it is acidophilic
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In situ hybridization
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Complementary RNA labeled= binds transcripts
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Antibodies(immunohistochemistry)
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1. Binds a specific protein
2. Antibody linked to tag/marker -fluorescent marker -chromagen -gold -easier to attach to Ab than to protein 3. Amplify signal -tagged 2nd Ab -many can bind to 1st one 4. Multiple tags |
