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8 Cards in this Set

  • Front
  • Back
what do most biochemical studies that involve the measurement of a biological activity require ?
the normalization of that activity to the protein content
what is of particular importance when proteins are being purified or different samples are being compared?
the specific activity of a particular enzyme activity
the most utilized methods to assay total protein relay on ...
the reduction of copper in the presence of a chromogenic reagent
______of the protein estimation is the most widely used and accepted method for accurate estimation of protein concentration
Lowry's method
Lowry's method of a combination of what two reactions?
biuret reaction and folin-ciocalteau reaction
what happens in the first step of lowry's method?

and in the second step?
the protein binds to copper in alkaline medium and produces Cu++

Cu++ catalyses the oxidation of aromatic amino acid by reducing phosphomolybdotungstate to heteropolymolybdanum blue
Lowry's reaction produces a strong blue, which predominately depends on ____&_____ content of protein and to a lesser extent ___&____in protein
tyrosine and tryptophan

cysteine and other residues
what are the five materials?
cell culture dishes at confluency
(joining of two or more, flowing together)

cell lysis buffer

cell scrapper

1.5mL micro centrifuge tube

37 C incubator