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8 Cards in this Set
- Front
- Back
what do most biochemical studies that involve the measurement of a biological activity require ?
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the normalization of that activity to the protein content
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what is of particular importance when proteins are being purified or different samples are being compared?
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the specific activity of a particular enzyme activity
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the most utilized methods to assay total protein relay on ...
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the reduction of copper in the presence of a chromogenic reagent
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______of the protein estimation is the most widely used and accepted method for accurate estimation of protein concentration
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Lowry's method
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Lowry's method of a combination of what two reactions?
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biuret reaction and folin-ciocalteau reaction
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what happens in the first step of lowry's method?
and in the second step? |
the protein binds to copper in alkaline medium and produces Cu++
Cu++ catalyses the oxidation of aromatic amino acid by reducing phosphomolybdotungstate to heteropolymolybdanum blue |
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Lowry's reaction produces a strong blue, which predominately depends on ____&_____ content of protein and to a lesser extent ___&____in protein
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tyrosine and tryptophan
cysteine and other residues |
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what are the five materials?
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cell culture dishes at confluency
(joining of two or more, flowing together) cell lysis buffer cell scrapper 1.5mL micro centrifuge tube 37 C incubator |