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18 Cards in this Set

  • Front
  • Back

Semiconservative replication

Both resulting strands contain a new strand and an original template strand

Molecular forces holding DNA together

Backbone = covalent bonds


Two strands = hydrogen bonds

Steps from DNA to protein

Transcription


Translation

non encoding RNA

Ribosomal RNA (rRNA)


Translational RNA (tRNA)


Various Non-coding RNAs

RNA interactions and folding

RNA to self (tRNA)


RNA to protein (rRNA)


Catalytic activity (microRNA)

Protein structural levels

primary (AA sequence)


secondary (B and a folds)


Tertiary (3D)


Quaternary (multiple domain incorporation)

What came first, the DNA/RNA or the protein?

Both depend on each other

Membrane constituents

Phospholipids


Proteins


Carbohydrates


Cholesterol

Minimum number of genes essential for living

400 genes

Organotrophs

Consume carbon for energy

Phototrophs

Create carbon sources from light

Lithotrophs

Use inorganic materials to create carbon sources

Three domains of life

Bacteria


Archaea


Eukaryotes

Bacterial genetic innovation (4)

Intragenic mutation


Gene Duplication


DNA segment shuffling


Horizontal or lateral transfer

Orthologous genes

Ancestral organism gives rise to two different species

Paralogous genes

Different genes in same species among different organisms

Conserved genetic families

Translation


Transcription


Replication, Combination, Repair


Cell processes and signalling


Cell cycle control, mitosis, meiosis


Defense mechanisms


Signal transduction


Cell wall/membrane biogensis


Intracellular trafficking and secretion


Post-translational modification, protein turnover, chaperones

Bacterial transcription techniques

Origin of replication


Transcription goes both ways along DNA loop


Terminus of replication for both strands