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27 Cards in this Set
- Front
- Back
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Give one disadvantage of using electron microscopy? |
The material is always dead and fixed |
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What is the definition of "optical resolution limit?" |
The minimum distance that allows recognition of object details |
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Smaller wavelength = better resolution, true or false |
true |
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What wavelength of light does light microscopy use? |
390nm-700nm |
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What kind of lens is used to focus the light? |
Glass |
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What is the resolution limit of light microscopy? |
around 200nm |
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What is the main advantage of using light microscopy? |
The cells are alive |
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What wavelength of light is used in electron microscopes |
0.0025nm |
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What kind of lens is used to focus the beam? |
Electromagnetic lens |
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What is the resolution limit of an electron microscope? |
0.05nm |
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What is the main advantage of using electron microscopy? |
High resolution |
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What is the difference in the image between a scanning electron microscope and a transmission electron microscope? |
Scanning electron microscope can give almost 3D images Transmission electron microscope give a cross section |
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What is the advantage of amalgamating many images? |
It allows for the reconstruction of ultra-structure
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What is fluorescence? |
It is the emission of light by a substance that has absorbed light |
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Is the wavelength of light emitted at a higher or lower wavelength than the excitation? |
at a higher wavelength |
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What can fluorescent microscopy allow the visualization of? |
Single molecules |
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What was the first fluorescent protein to be isolated? |
GFP (green fluorescent protein) |
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What animal was GFP isolated from? |
Jelly fish (aequorea victoria) |
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Give an example of a dynamic that GFP can help to visualize? |
micro-tubules |
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By using two different fluorescent proteins what can be achieved? |
Multiple proteins can be analysed at the same time |
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What does FRAP stand for? |
Fluorescent recovery after photo-bleaching |
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What can FRAP be used to prove? |
It can reveal differences in membrane fluidity and protein mobility |
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What does FLIP stand for? |
Fluorescent loss in photo-bleaching |
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What can FLIP be used to prove? |
It can be used to prove protein cycling between different organelles |
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What is photo-activation? |
Fluorescent proteins that become visible after laser radiation |
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What is the wavelength of light needed to activate the protein |
around 400nm |
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What wavelength of light is emitted by the protein? |
488nm |
