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12 Cards in this Set
- Front
- Back
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culture conditions, purpose:
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to see how cells behave under a certain set of conditions
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experimental input:
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cell type, nutrients, matrix, additives, mechanical stresses, presence/absence of other cells
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experimental output
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intracellular structure, stasis/division/apoptosis, migration, attachement/spreading/polarization, differentiation, proteins, gene expression
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actin staining
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wash w/ PBS, fix w/ glutaraldehyde, permeabilize cell membranes w/ Triton-X100, stain actin w/ phalloidin
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Fluorescein
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fluorescent dye - excited by blue, emits green
dyes F-actin |
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Rhodamine
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fluorescent dye - excited by green-yellow, emits red
dyes mitochondria |
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DAPI
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fluorescent dye - excited by violet, emits blue
dyes nucleus |
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glutaraldehyde
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fixes cells (causes actin to cross-link)
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Triton-X100
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permeabilizes cell membranes
surfactant creates holes in membrane, allowing stain to penetrate inside |
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Phalloidin
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antibody that binds to F-actin.
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Rhodamine
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fluorescent "tag" that binds to phalloidin. Emits red.
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why can't we store fluorophores in the light?
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photobleaching
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