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10 Cards in this Set
- Front
- Back
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pGlo
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plasmid that is transformed into bacterial cells. encodes bla, GFP, and araC genes
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bla gene
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encodes beta lactamase
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beta lactamase
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protein that breaks down ampicilin
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ampicillin
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antibiotic that kills ecoli
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GFP gene
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green flourescent protein gene. encodes GFP
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arabinose PBAD promoter
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induces GFP protein production, transcription allowed in presence of arabinose and inhibited in absence
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araC gene
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regulates GFP production through PBAD promoter
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araC protein
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binds to PBAD promoter and restricts GFP production
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Arabinsoe
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inducer sugar
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If bla gene encodes beta lactamase
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ampicillin resistance should be observed
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