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16 Cards in this Set
- Front
- Back
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what is endocytosis
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when cells internalize different particles and solutes from their environment
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what are the 3 forms of endocytosis ?
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phagocytosis
pinocytosis receptor mediated endocytosis |
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____ is the ingestion of MOLECULES upon interaction with specific receptors on the cell surface
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receptor mediated endocytosis
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____ is the ingestion of MOLECULES upon interaction with specific receptors on the cell surface
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receptor mediated endocytosis
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which two mechanism share a clathrin based mechanism ?
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receptor mediated and pinocytosis
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what is clathrin?
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a protein composed of 3 light and 3 heavy polypeptide chains that together form a 3 legged structure called a triskelion
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this complex forms a regular lattice that coats the outsides of vesicles, facilitating invagination and pinching off the membrane and enclosure of particles / proteins to be transported
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triskelion
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why do unicellular organisms used phagocytosis?
why do higher organisms use phagocytosis |
to obtain nutrients
degradation of pathogens and cellular debris immune response |
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higher organisms have developed specialized phagocytic cells such as __- __ and ___
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macrophages dentric cells and neutrophils
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most mechanisms of phagocytosis share what in common
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particle internalization is iniated by the interaction of surface receptors (on the phagocytes) with their cognate ligands (on the particles)
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after particle initiation this leads to _____ at the site of ingestion, resulting in complete wrapping of particles and their release in the cytoplasm as novel membrane-bound organelles: the ____
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cytoskeleton rearrangement or polymerizaton of actin
phagosomes |
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once phagosomes are in the cytoplasm they acquire different proteins, including _____, during maturation and ultimately fuse with ____ generating the _____
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Rab GTPases
ultimately fuse with acidic lysosome phagolysosome |
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what happens in phagolysosome?
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phagocytosed material is degraded
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we did we do in this lab?
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compare the phagocytosis of red fluorescent tagged pseudomonas bacteria by wild type J774 Eclone cells and Rab 5 transfected J774 Eclone cells
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the j774 cell line is established from a _____
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a tumor that arose in a female BALB /c mouse
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what is DAPI used for?
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flurescent stain
to stain DNA |
