Cell Bio Lab Final Cell Staining And Counting

Front 1

what is a counting chamber

what is the most widely used type of chamber

what was is originally designed for

Back 1

a device used for determining the number of cell per unit volume of a suspension

hemocytometer

for performing blood cell counts

Front 2

coverslips for counting chambers are specially made and are _____ than those for conventional microscopy, since they must be ____

Back 2

thicker

heavy enough to overcome the surface tension of a drop of liquid

Front 3

when is the coverslip placed over counting surface?

Back 3

prior to putting on the cell suspension

Front 4

the suspension is introduced into one of the ____ shaped wells with a ____ or other type of pipette. the area under the coverslip fills by ______

Back 4

V shaped
pasteur
capillary action

Front 5

each square has a surface area of one ____

and the depth of the chamber is ___

Back 5

mm squared (little two)

0.1 mm

Front 6

suspension should be dilute enough so that the cells do not ______

Back 6

overlap each on the grid, and should be uniformly distributed

Front 7

suppose you count a total of 125 cells in 5 squares

each square has a surface area of 1 mm squared and depth of 0.1mm giving you a volume of 0.5mm cubed

you get 250 ____
and then you multiply by 1000 to determine ____ per ___

Back 7

cells /mm cubed
cell count per ml (250,000)

Front 8

why would you need to dilute a cell suspension?

in the case that you do dilute a cell suspension you will need the multiply _____ by _____

Back 8

to get the cell density low enough for counting

final count by the dilution factor

Front 9

what is cell viability ?

what is the most widely used technique for calculating cell viability ?

Back 9

the ratio of live cells to total cells

trypan blue staining

Front 10

what is trypan blue?

Back 10

dye
used to determine the viability of a cell

Front 11

living cells ___ the dye, whereas dead cells ____

Back 11

exclude
will take up the blue dye

Front 12

will counted the number of cells from ___ flasks

Back 12

T25

Front 13

in this lab we used a ___mm cell culture dish

Back 13

35

Front 14

to determine the percentage of viable cells you divide the number of ______ cells by the _____ and then _____

Back 14

unstained cells by the total number of cells and multiply by 100

# of unstained cells / total cells X 100= percent viable cells

Front 15

why is it important to quantify the amount of cytosolic protein in cells

Back 15

to compare samples

Front 16

why do we use cell lysate?

Back 16

to get protein inside