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32 Cards in this Set
- Front
- Back
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What are the two requirements for intra-golgi transport?
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cytosol and ATP
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What is GTP gamma ase?
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non-hydrolysing GTP, used to test transportation
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When is Cop 1 used?
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intraGolgi and ER
- needed for default pathway |
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When is COPII used?
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ER to cisGolgi
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What is the structure of Coatamer, a COP complex?
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- seven polypeptides mediate vesicle budding
- membrane bound and cytoplasmic pools - GTP needed to bind COP to Golgi membrane, GTP needed to release it as well. Needs ARF |
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What are the two requirements for intra-golgi transport?
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cytosol and ATP
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What is GTP gamma ase?
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non-hydrolysing GTP, used to test transportation
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When is Cop 1 used?
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intraGolgi and ER
- needed for default pathway |
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When is COPII used?
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ER to cisGolgi
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What is the structure of Coatamer, a COP complex?
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- seven polypeptides mediate vesicle budding
- membrane bound and cytoplasmic pools - GTP needed to bind COP to Golgi membrane, GTP needed to release it as well. Needs ARF |
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What is ARF?
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A dynamic adaptor protein that mediates COP binding to donor membranes
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What does ARF hydrolyze? How?
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GTP with its myristoylated tail.
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What binds ARF to a donor membrane using GTP?
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the myristoylated tail
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What is GNRP? What does it do?
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1. guanine-nucleotide exchange protein.
2. stimulates the exchange of GDP to GTP on ARF |
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How do COP proteins bind to a PM?
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Using GTP-bound ARF
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How does ARF- GTP convert back to ARF GDP?
What causes this change? |
ARF GTPase
Interaction with target membrane |
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ARF is bidirectional or unidirectional?
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Unidirectional.
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Which ARF proteins are used in the Golgi and Secretion?
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ARF 1,3
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Which ARF are used in the TGN?
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ARF 2,4,5
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Which ARF are used in PM and endosomes?
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ARF 6
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What are two of ARF's functions?
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coat recruitment, lipid metabolism
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Wjat are compartment specific signalosomes linked to?
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phosphoinositides
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What are the main proteins used in fusion?
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Snare, RABS,, SNAPS, NSF
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What are SNARES?
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a class of integral membrane protein present on donor and target membrane compartments.
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Which SNARE is present on the donor vesicle?
The Target compartment? |
v-SNARE
t-SNARE |
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Which proteins make sure that SNARE is matched with its correct counterpar?
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RABs
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Where is the RAB specificity located?
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The divergent C-terminal sequences, not the GTP-binding domains
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Do RABS bind ATP or GTP?
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GTP
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What happens after RABs do their jobs?
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After GTP hydrolysis, they are released to the cytosol for another round of action. Vesicle is ready for fusion
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What is the RAB effector?
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It tethers the two Rabs on the surfaces together so that SNAREs can bind
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What is NSF?
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N-ethylmaleidide-sensitive fusion protein
-ATPase -Somehow used in fusion - might disseble the proteins - reactivate SNAREs for next round |
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What is SNAP?
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soluble NSF attachment proteins
-for attaching NSF to membrane |
