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77 Cards in this Set

  • Front
  • Back
Harris hematoxylin is used on tissue sections to stain:
nuclei
Ripening of hematoxylin is a process of:
oxidation
The active staining ingredient in ripened hematoxylin solutions is:
hematein
The most important step in regressive hematoxylin staining is:
differentiation in acid-alcohol
Hematein is formed in Mayer hematoxylin solution by the addition of:
sodium iodate
Mordants are used to:
link tissue constituents more closely to the dye
During H&E staining, if ammonia is incompletely removed by washing, the result may be:
poor staining with eosin
The combination of a dye and a mordant is called:
dye lake
Mercuric oxide (or sodium iodate) is used in Harris Hematoxylin to:
form hematein
DNA can be demonstrated with:
the Feulgen reaction
All of the following are aluminum hematoxylins EXCEPT:

a) Delafield
b) Harris
c) Mayer
d) Weigert
d) Weigert
Which of the following hematoxylins is not readily decolorized with acidic solutions?

a) Harris
b) Delafield
c) Weigert
d) Verhoeff
c) Weigert
Natural resins are rarely used for mounting sections today because they:
are inherently acidic
Which of the following stains requires mounting with aqueous mounting media?

a) Fite
b) Oil Red O
c) Grocott
d) H&E
b) Oil Red O
The mordant in Weigert hematoxylin is:
iron
Acetic acid is added to Harris hematoxylin to:
make nuclear staining more specific
A dye that may be substituted for hematoxylin in routine staining is:
celestine blue
Which of the following is a good example of a polychrome stain?

a) alcian blue
b) Congo Red
c) Giemsa
d) Kinyoun
c) Giemsa
Which of the following is stained rose by the methyl green-pyronin (MGP) technique?

a) heterochromatin
b) DNA
c) RNA
d) Golgi apparatus
c) RNA
The chemical group in dyes that confers the property of color is called a:
chromophore
An auxochrome is a/an:
ionizing group present in dyes
Which of the following is formed when hematoxylin is subjected to the action of sodium iodate?

a) a dye lake
b) hematein
c) hematin
d) a polychrome
b) hematein
Heterochromatin is stained by:
hematoxylin
Which of the following techniques is an example of dye absorption?

a) H&E
b) methyl green-pyronin
c) Feulgen
d) oil red O
d) oil red O
Most cytoplasmic proteins are:
acidophilic
Differentiating in the H&E stain is an example of using:
a weak acid
A tissue component that takes up a cationic dye is said to be:
basophilic
Into which of the following dye categories does eosin fall?

a) polychromatic
b) cationic
c) anionic
d) amphoteric
c) anionic
For the best cytoplasmic staining, the pH of the eosin should be between:
4.6 to 5.0
Ethylene glycol functions in Gill hematoxylin solutions to:
prevent surface scum
Which of the following hematoxylin solutions will stain the mucin in goblet cells?

a) Gill
b) Mayer
c) Harris
d) Weigert
a) Gill
The Feulgen reaction demonstrates:
DNA only
The Feulgen reaction is unsatisfactory on tissue fixed in:
Bouin solution
Romanowsky-type stains are combinations of:
one acid and one basic dye
Nuclear staining is made more selective by adding which of the following to hematoxylin solutions?

a) sodium citrate
b) chloral hydrate
c) ethylene glycol
d) acetic acid
d) acetic acid
Hematoxylin is converted to a dye lake in the Mayer formula by adding which of the following to the solution?

a) sodium iodate
b) citric acid
c) ammonium aluminum sulfate
d) chloral hydrate
c) ammonium aluminum sulfate
A large quantity of Delafield hematoxylin stock solution must be maintained because of:
slow hematein formation
To link hematoxylin to tissue DNA, which of the following must be added?

a) acid
b) oxidizer
c) stabilizer
d) mordant
d) mordant
Hematoxylin is an effective stain when it:

a) can only be used progressively
b) contains chromophoric groupings
c) influences the isoelectric point of proteins
d) is polychromed
b) contains chromophoric groupings
Romanowsky-type stains are usually preferred for the demonstration of:
leukocytes
For the most transparency and clarity when viewing well-stained microscopic sections, the refractive index of the mounting medium should be:
near that of the tissue
Synthetic resins are preferred over natural resins because the synthetic resins:
preserve the intensity of the stains with time
Aqueous mounting media have an index of refraction that is:
significantly below that of the tissue
A decrease in section transparency can be caused by using:
mounting medium that has become to thick
Fixation with NBF will cause tissue cytoplasm to:

a) take up more hematoxylin
b) take up more eosin
c) be more difficult to blue
d) resist acid decolorization
a) take up more hematoxylin
If the pH of the staining solution is between 4.6 and 5.0 eosin combines with which of the following chemical groups?

a) COO⁻
b) NH₃⁺
c) PO₃⁻
d) N=N
b) NH₃⁺
Which of the following can be added to an aqueous mounting medium to prevent bleeding of analine dyes into surrounding medium?

a) cane sugar
b) Canada balsam
c) glycerine
d) gelatin
a) cane sugar
The Giemsa stain is most satisfactory if the pH is between:
6.4 and 6.9
Which of the following hematoxylin solutions must be used within a few days after preparation?

a) Gill
b) Delafield
c) Harris
d) Weigert
d) Weigert
An organelle that causes the cytoplasm to show increased basophilia is the:
rough endoplasmic reticulum
T/F: Basic dyes have a negative charge.
-False

-basic dyes have a positive charge, and bind with negatively charged particles
T/F: Oxidizers are sometimes used for differentiation.
true
T/F: Eosin is differentiated by the dehydrating alcohols.
true
T/F: An increase or decrease in the pH of the staining solution can alter staining by changing tissue and/or dye changes.
true
T/F: Staining is influenced by the fixative used.
true
T/F: Ferric chloride is both mordant and oxidizer.
true
T/F: Delafield hematoxylin is an iron hematoxylin.
-false

-Weigert hematoxylin is an iron hematoxylin
T/F: An increase in temperature usually increases the rate of staining.
true
T/F: Frozen sections can be stained with thionin.
true
T/F: Resinous mounting media have an index of refraction much lower than that of the tissue.
false
T/F: Resinous mounting media are usually dissolved in toluene or xylene.
true
T/F: "The more, the better" is a good practice for applying mounting medium.
false
T/F: The nucleolus of plasma cells is stained green with the methyl green-pyronin technique.
-false

-DNA is stained green
T/F: Bouin solution is a good fixative for tissue to be stained with the Feulgen reaction.
-false

-bouin solution should not be used with the Feulgen reaction
Microscopic examination of an H&E-stained section shows nuclei with well-defined chromatin patterns, crisp nuclear membranes, and very pale pink staining of the cytoplasm and erythrocytes. These results indicate:
a probable pH problem with the eosin
An H&E-stained section shows reddish-brown stained nuclei, pink cytoplasm, and bright rose red erythrocytes. These results indicate:
the use of overripened hematoxylin
H&E-stained sections show very uneven staining with some areas of the slide well stained and other areas unstained. These results indicate that most likely the:
paraffin was not completely removed
While staining a rack of sections, it is noted that the water following rehydrating alcohols turns very cloudy. This can most likely be corrected by:
changing the alcohols
H&E-stained sections reveal brown pigment-like stippling and rare glossy black nuclei. This most likely has been caused by a mounting medium that:
was applied after letting the slide dry
H&E-stained sections of liver show very dark nuclei and some blue staining in the cytoplasm. This is most likely caused by:
inadequate differentiation of the hematoxylin
H&E-stained sections of small intestine reveal muscle, collagen, and red blood cells all stained the same shade of pink. This indicates:
poor differentiation of the eosin
A blue-black precipitate is seen on H&E-stained sections. This could probably be prevented in the future by:
filtering the hematoxylin
Water bubbles are seen microscopically on H&E-stained sections. This could probably be prevented in the future by:
making sure that the dehydration step is complete
Microscopic examination of an H&E-stained section of kidney is very difficult. Some areas of the tissue cannot be brought into focus, while other areas show excellent detail. This is most likely due to:
mounting medium on top of the cover glass
H&E-stained sections show hazy blue nuclei, but recuts from tissue processed a week previously and stained in the same basket show excellent nuclear staining. One possible cause of the problem is:
the use of too much heat during processing
A Feulgen stain is requested on a section of Bouin-fixed lymph node. The best course of action is to:
ask if there is tissue in another fixative
Sections for special stains have been accidentally stained with hematoxylin. To remove the hematoxylin, place the sections in:
acid alcohol