Carson's Carbohydrate Staining

Front 1

Glycogen is best demonstrated by the use of:

Back 1

PAS with and without diastase

Front 2

Amyloid can be demonstrated with:

Back 2

Congo red

Front 3

The Schiff reaction demonstrates:

Back 3

aldehydes

Front 4

A good control for the Mayer mucicarmine stain is:

Back 4

appendix

Front 5

A good control for glycogen is:

Back 5

liver

Front 6

To increase the specificity for amyloid, Congo red stains should be examined by which microscope?

Back 6

polarizing

Front 7

Of the following, which is the best fixative for glycogen?

a) Zenker
b) Orth
c) acetic acid
d) absolute alcohol

Back 7

d) absolute alcohol

Front 8

Periodic acid is used in the PAS technique as a/an:

Back 8

oxidizer

Front 9

The crystal violet stain for amyloid is a/an:

Back 9

polychromatic stain

Front 10

Acid mucopolysaccharides are demonstrated by:

Back 10

alcian blue

Front 11

The reliability of the Schiff reagent may be checked by adding which of the following to a small aliquot of the schiff solution?

a) sodium iodate
b) formaldehyde
c) potassium metabisulfite
d) diastase

Back 11

b) formaldehyde

Front 12

A fluorescent dye used for the demonstration of amyloid is:

Back 12

thioflavin T

Front 13

The alcian blue stain performed at pH 1.0 demonstrates:

Back 13

sulfated acid mucopolysaccharidess

Front 14

Substances stained positively with the colloidal iron will be:

Back 14

blue

Front 15

Schiff reagent is a/an:

Back 15

reduced solution of basic fuchsin

Front 16

Diastase digestion increases specificity for:

Back 16

glycogen

Front 17

Colloidal iron is used for the demonstration of:

Back 17

acid mucopolysaccharides

Front 18

A mucicarmine stain would be most helpful in the diagnosis of:

Back 18

adenocarcinoma

Front 19

Adjacent sections are stained with PAS, one with and one without diastases digestion. A positive result on the one without digestion and a negative result on the one with digestion indicates the presence of:

Back 19

glycogen

Front 20

T/F: Glucose, sucrose, and other oligopolysaccharides can be demonstrated easily in tissue sections.

Back 20

false

Front 21

T/F: Hyaluronidase is used to digest some connective tissue mucins.

Back 21

true

Front 22

T/F: The routine alcian blue stain is done at pH 1.5.

Back 22

false

Front 23

T/F: Good Schiff reagent should be light pink.

Back 23

false

Front 24

T/F: The end product in the colloidal iron method is Prussian blue.

Back 24

true

Front 25

T/F: Amyloid shows a yellow birefringence following staining with Congo red.

Back 25

false

Front 26

T/F: Glutaraldehyde is one of the recommended fixatives for the PAS reaction.

Back 26

false

Front 27

T/F: The Schiff reaction may show false positively following chromate-containing fixatives.

Back 27

false

Front 28

T/F: Glycogen-containing tissue fixed in Bouin solution may show resistance to diastase digestion.

Back 28

true

Front 29

T/F: The addition of acid to the crystal violet staining solution reduces background staining.

Back 29

true

Front 30

T/F: 4um to 6um sections are recommended for crystal violet stains.

Back 30

false

Front 31

What reagents are used to show acid mucopolysaccharides?

Back 31

-alcian blue, pH 1.0

-alcian blue, pH 2.5

-colloidal iron

Front 32

What reagents are used to show amyloid?

Back 32

-Congo red

-crystal violet

-thioflavin T

Front 33

What reagents are used to show carboxylated acid mucopolysaccharides?

Back 33

-alcian blue, pH 2.5

-colloidal iron

Front 34

What reagents are used to show glycogen?

Back 34

-diastase

-periodic acid

-Schiff reagent

Front 35

What reagents are used to show neutral polysaccharides?

Back 35

-periodic acid

-Schiff reagent

Front 36

Which reagents are used to show sulfated (only) acid mucopolysaccharides?

Back 36

-alcian blue, pH 1.0

Front 37

No staining of the glomerular basement membrane can be seen microscopically on a control section of kidney. This may be the result of:

Back 37

inadequate oxidation

Front 38

Marked nonspecific background staining is noted on a section stained with the PAS technique. This could be the result of:

Back 38

fixation with glutaraldehyde

Front 39

Very weak staining is noted on a PAS-stained control section of liver. One problem-solving action is to:

Back 39

check the Schiff reagent with formaladehyde

Front 40

Sections of small intestine show orange goblet cells that are partially obscured by the yellow background. This is most likely the result of:

Back 40

overstaining with metanil yellow

Front 41

Control sections stained with Congo red show only yellow, and no green, birefringence. This could probably be corrected in the future by:

Back 41

ensuring that the sections are cut at 8um to 10um